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Rat Recombinant Monoclonal XCT antibody. Carrier free. Suitable for WB, ICC/IF, IP and reacts with Human samples.

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Images

Immunoprecipitation - Anti-xCT antibody [3A12] (BSA and Azide free) (AB302919), expandable thumbnail
  • Western blot - Anti-xCT antibody [3A12] (BSA and Azide free) (AB302919), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-xCT antibody [3A12] (BSA and Azide free) (AB302919), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Rat
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFIP
Human
Tested
Not recommended
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Heterodimer with SLC3A2, that functions as an antiporter by mediating the exchange of extracellular anionic L-cystine and intracellular L-glutamate across the cellular plasma membrane (PubMed:11133847, PubMed:11417227, PubMed:14722095, PubMed:15151999, PubMed:34880232, PubMed:35245456, PubMed:35352032). Provides L-cystine for the maintenance of the redox balance between extracellular L-cystine and L-cysteine and for the maintenance of the intracellular levels of glutathione that is essential for cells protection from oxidative stress (By similarity). The transport is sodium-independent, electroneutral with a stoichiometry of 1:1, and is drove by the high intracellular concentration of L-glutamate and the intracellular reduction of L-cystine (PubMed:11133847, PubMed:11417227). In addition, mediates the import of L-kynurenine leading to anti-ferroptotic signaling propagation required to maintain L-cystine and glutathione homeostasis (PubMed:35245456). Moreover, mediates N-acetyl-L-cysteine uptake into the placenta leading to subsequently down-regulation of pathways associated with oxidative stress, inflammation and apoptosis (PubMed:34120018). In vitro can also transport L-aspartate (PubMed:11417227). May participate in astrocyte and meningeal cell proliferation during development and can provide neuroprotection by promoting glutathione synthesis and delivery from non-neuronal cells such as astrocytes and meningeal cells to immature neurons (By similarity). Controls the production of pheomelanin pigment directly (By similarity).

Alternative names

Cystine/glutamate transporter, Amino acid transport system xc-, Calcium channel blocker resistance protein CCBR1, Solute carrier family 7 member 11, xCT, SLC7A11

Recommended products

Rat Recombinant Monoclonal XCT antibody. Carrier free. Suitable for WB, ICC/IF, IP and reacts with Human samples.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
3A12
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

ab302919 is a carrier free version of Anti-xCT antibody [3A12] ab300667.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunoprecipitation - Anti-xCT antibody [3A12] (BSA and Azide free) (ab302919), expandable thumbnail

    Immunoprecipitation - Anti-xCT antibody [3A12] (BSA and Azide free) (ab302919)

    This data was developed using Anti-xCT antibody [3A12] ab300667, the same antibody clone in a different buffer formulation.xCT was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug with Anti-xCT antibody [3A12] ab300667 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-xCT antibody [3A12] ab300667 at 1/1000 dilution. Goat Anti-Rat IgG (H+L), HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) was used at 1/10000 dilution. Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug Lane 2: Anti-xCT antibody [3A12] ab300667 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate Lane 3:Rat IgG2a, kappa monoclonal- Isotype Control (Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control ab18450) instead of Anti-xCT antibody [3A12] ab300667 in HepG2 whole cell lysate Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 seconds

  • Western blot - Anti-xCT antibody [3A12] (BSA and Azide free) (ab302919), expandable thumbnail

    Western blot - Anti-xCT antibody [3A12] (BSA and Azide free) (ab302919)

    This data was developed using Anti-xCT antibody [3A12] ab300667, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration: 5% NFDM/TBST. Low expression: SW480 and colo 205 (PMID: 31000598). Samples are non-boiled as boiling may cause protein aggregation. The expression profile observed is consistent with what has been described in the literature ( PMID: 32188872). Exposure time: 26 seconds

    All lanes: Western blot - Anti-xCT antibody [3A12] (Anti-xCT antibody [3A12] ab300667) at 1/1000 dilution

    Lane 1: A549 (human lung carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 3: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 4: SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 5: COLO 205 (human colon; derived from metastatic site: ascites epithelial), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/10000 dilution

    Observed band size: 55 kDa, 35 kDa

    Exposure time: 26s

  • Immunocytochemistry/ Immunofluorescence - Anti-xCT antibody [3A12] (BSA and Azide free) (ab302919), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-xCT antibody [3A12] (BSA and Azide free) (ab302919)

    This data was developed using Anti-xCT antibody [3A12] ab300667, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labelling xCT with Anti-xCT antibody [3A12] ab300667 at 1/50 (21.18 ug/ml) dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membraneous and cytoplasmic staining in A549 cell line.Low expression: colo 205 (PMID: 31000598). is observed. Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 10 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/ml dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com