Rabbit Polyclonal XCT antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 9 publications.
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- Cell death discovery 9:3312023In vitro CRISPR screening uncovers CRTC3 as a regulator of IFN-γ-induced ferroptosis of hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Li,Tao Xing,Yiran Chen,Weiran Xu,Bo Fan,Gaoda Ju,Jing Zhao,Li Lin,Cihui Yan,Jun Liang,Xiubao RenPubMed 37666810
- Journal of gastrointestinal oncology 14:1715-17252023CircRHOT1 restricts gastric cancer cell ferroptosis by epigenetically regulating GPX4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuan Wang,Daniel Adam Breadner,Ke Deng,Jing NiuPubMed 37720433
- Nutrients 15:2023Hydroxysafflor Yellow A Alleviates Acute Myocardial Ischemia/Reperfusion Injury in Mice by Inhibiting Ferroptosis via the Activation of the HIF-1α/SLC7A11/GPX4 Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChaowen Ge,Yuqin Peng,Jiacheng Li,Lei Wang,Xiaoyu Zhu,Ning Wang,Dongmei Yang,Xian Zhou,Dennis ChangPubMed 37571350
- Fundamental & clinical pharmacology 37:1129-11382023Novel ginsenoside monomer RT4 promotes colitis repair in mice by regulating miR-144-3p/SLC7A11 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao Li,Liu Han,Binfen Hou,Yanhong JiPubMed 37350460
- Discover. Oncology 14:852023Tetrandrine citrate suppresses lung adenocarcinoma growth via SLC7A11/GPX4-mediated ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaocong Mo,Di Hu,Kaisheng Yuan,Juyu Luo,Cheng Huang,Meng XuPubMed 37266741
- Experimental & molecular medicine 55:939-9512023Mesenchymal stem cell-derived extracellular vesicles protect against abdominal aortic aneurysm formation by inhibiting NET-induced ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Chen,Yuting Liu,Zheyu Wang,Leiyang Zhang,Yi Xu,Yinan Li,Lan Zhang,Guiming Wang,Shuofei Yang,Guanhua XuePubMed 37121969
- Diabetic medicine : a journal of the British Diabetic Association 40:e150312023Bone marrow stromal cell-derived exosomal circular RNA improves diabetic foot ulcer wound healing by activating the nuclear factor erythroid 2-related factor 2 pathway and inhibiting ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuehao Chen,Xi Li,Hua Liu,Da Zhong,Ke Yin,Yusheng Li,Lemei Zhu,Can Xu,Mingqing Li,Chenggong WangPubMed 36537855
- Journal of experimental & clinical cancer research : CR 42:62023Targeting fatty acid synthase modulates sensitivity of hepatocellular carcinoma to sorafenib via ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Li,Wenjuan Yang,Yuanyuan Zheng,Weiqi Dai,Jie Ji,Liwei Wu,Ziqi Cheng,Jie Zhang,Jingjing Li,Xuanfu Xu,Jianye Wu,Mingwei Yang,Jiao Feng,Chuanyong GuoPubMed 36604718
- Redox biology 50:1022572022T lymphocyte-derived extracellular vesicles aggravate abdominal aortic aneurysm by promoting macrophage lipid peroxidation and migration via pyruvate kinase muscle isozyme 2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuohui Dang,Tianrun Li,Dongmin Yang,Guangxin Yang,Xing Du,Juan Yang,Yutong Miao,Lulu Han,Xiaolong Ma,Yuwei Song,Bo Liu,Xuan Li,Xian Wang,Juan FengPubMed 35149342
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
Heterodimer with SLC3A2, that functions as an antiporter by mediating the exchange of extracellular anionic L-cystine and intracellular L-glutamate across the cellular plasma membrane (PubMed:11133847, PubMed:11417227, PubMed:14722095, PubMed:15151999, PubMed:34880232, PubMed:35245456, PubMed:35352032). Provides L-cystine for the maintenance of the redox balance between extracellular L-cystine and L-cysteine and for the maintenance of the intracellular levels of glutathione that is essential for cells protection from oxidative stress (By similarity). The transport is sodium-independent, electroneutral with a stoichiometry of 1:1, and is drove by the high intracellular concentration of L-glutamate and the intracellular reduction of L-cystine (PubMed:11133847, PubMed:11417227). In addition, mediates the import of L-kynurenine leading to anti-ferroptotic signaling propagation required to maintain L-cystine and glutathione homeostasis (PubMed:35245456). Moreover, mediates N-acetyl-L-cysteine uptake into the placenta leading to subsequently down-regulation of pathways associated with oxidative stress, inflammation and apoptosis (PubMed:34120018). In vitro can also transport L-aspartate (PubMed:11417227). May participate in astrocyte and meningeal cell proliferation during development and can provide neuroprotection by promoting glutathione synthesis and delivery from non-neuronal cells such as astrocytes and meningeal cells to immature neurons (By similarity). Controls the production of pheomelanin pigment directly (By similarity).
Alternative names
Cystine/glutamate transporter, Amino acid transport system xc-, Calcium channel blocker resistance protein CCBR1, Solute carrier family 7 member 11, xCT, SLC7A11
Recommended products
Rabbit Polyclonal XCT antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 9 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
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4 product images
Western blot - Anti-xCT antibody (ab216876)
Dilution buffer: 0.01% sodium azide in PBS.
xCT processing caused by dimerization, glycosylation, and/or phosphorylation may cause other bands.
All lanes: Western blot - Anti-xCT antibody (ab216876) at 1/1000 dilution
Lane 1: A549 (human lung carcinoma cell line) whole cell lysate at 10 µg
Lane 2: HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 10 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
SecondaryAll lanes: anti-rabbit secondary DyLight 488 antibody at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 56 kDa
Flow Cytometry - Anti-xCT antibody (ab216876)
Flow cytometric analysis of human breast carcinoma cells labeling xCT with ab216876 at 1 μg/mL for 1 hour at 4°C. Rabbit IgG Antibody Dylight™488 Conjugated pre-adsorbed secondary antibody was used at 1 μg/mL for one hour on ice.
Left panels: Unstained.
Middle panels: Secondary antibody only.
Right panels: Primary and secondary antibodies.
Immunocytochemistry/ Immunofluorescence - Anti-xCT antibody (ab216876)
Immunofluorescent analysis of PC-3 (human prostate adenocarcinoma cell line) cells labeling xCT with ab216876 at 10 μg/mL for 1 hour, followed by anti-Rabbit IgG DyLight™ 488 Conjugated Preadsorbed secondary antibody at 5 μg/mL for 1 hour at RT (green). Cells were fixed with 100% methanol.
The nuclear counter stain is DAPI (blue).
A: DAPI
B: Primary followed by Anti-rabbit IgG Dylight™ 488
C: Merged images
D: Secondary only control
Western blot - Anti-xCT antibody (ab216876)
Exposure time: 15-30 seconds.Blocking buffer: 5% BSA.
Dilution buffer: 0.01% sodium azide in PBS.
xCT processing caused by dimerization, glycosylation, and/or phosphorylation may cause other bands.
All lanes: Western blot - Anti-xCT antibody (ab216876) at 1/1000 dilution
Lane 1: Recombinant Bcl3 (unrelated negative control) at 10 µg
Lane 2: NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
Lane 3: A549 (human lung carcinoma cell line) whole cell lysate at 10 µg
SecondaryAll lanes: anti-rabbit secondary HRP antibody at 1/70000 dilution
Predicted band size: 55 kDa
Observed band size: 56 kDa
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