Anti-xCT antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-xCT antibody (AB216876)

Immunofluorescent analysis of PC-3 (human prostate adenocarcinoma cell line) cells labeling xCT with ab216876 at 10 μg/mL for 1 hour, followed by anti-Rabbit IgG DyLight™ 488 Conjugated Preadsorbed secondary antibody at 5 μg/mL for 1 hour at RT (green). Cells were fixed with 100% methanol.

The nuclear counter stain is DAPI (blue).

A : DAPI

B : Primary followed by Anti-rabbit IgG Dylight™ 488

C : Merged images

D : Secondary only control

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-xCT antibody (AB216876)

Flow cytometric analysis of human breast carcinoma cells labeling xCT with ab216876 at 1 μg/mL for 1 hour at 4°C. Rabbit IgG Antibody Dylight™488 Conjugated pre-adsorbed secondary antibody was used at 1 μg/mL for one hour on ice.

Left panels : Unstained.

Middle panels : Secondary antibody only.

Right panels : Primary and secondary antibodies.

• WB

Supplier Data

Western blot - Anti-xCT antibody (AB216876)

Dilution buffer : 0.01% sodium azide in PBS.

xCT processing caused by dimerization, glycosylation, and/or phosphorylation may cause other bands.

All lanes:

Western blot - Anti-xCT antibody (ab216876) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 10 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

anti-rabbit secondary DyLight 488 antibody at 1/20000 dilution

Predicted band size: 55 kDa

Observed band size: 56 kDa

false

• WB

Supplier Data

Western blot - Anti-xCT antibody (AB216876)

Exposure time : 15-30 seconds.

Blocking buffer : 5% BSA.

Dilution buffer : 0.01% sodium azide in PBS.

xCT processing caused by dimerization, glycosylation, and/or phosphorylation may cause other bands.

All lanes:

Western blot - Anti-xCT antibody (ab216876) at 1/1000 dilution

Lane 1:

Recombinant Bcl3 (unrelated negative control) at 10 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Lane 3:

A549 (human lung carcinoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

anti-rabbit secondary HRP antibody at 1/70000 dilution

Predicted band size: 55 kDa

Observed band size: 56 kDa

false