Name: Anti-xCT antibody [EPR27115-64]
SKU: ab307601
Rating: 5 (1 reviews)

Anti-xCT antibody [EPR27115-64] ab307601 is a rabbit monoclonal antibody that is used in xCT western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Validated on the Leica BOND™ RX automated IHC staining platform for IHC
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- One antibody for all your xCT staining

Images

Western blot - Anti-xCT antibody [EPR27115-64] (AB307601), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	Flow Cyt (Intra)	ICC/IF	IHC-P	WB
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Expected	Expected	Expected	Tested	Tested
Rat	Expected	Expected	Expected	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Associated Products

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Target data

See full target information Cystine/glutamate transporter

Function

Heterodimer with SLC3A2, that functions as an antiporter by mediating the exchange of extracellular anionic L-cystine and intracellular L-glutamate across the cellular plasma membrane (PubMed:11133847, PubMed:11417227, PubMed:14722095, PubMed:15151999, PubMed:34880232, PubMed:35245456, PubMed:35352032). Provides L-cystine for the maintenance of the redox balance between extracellular L-cystine and L-cysteine and for the maintenance of the intracellular levels of glutathione that is essential for cells protection from oxidative stress (By similarity). The transport is sodium-independent, electroneutral with a stoichiometry of 1:1, and is drove by the high intracellular concentration of L-glutamate and the intracellular reduction of L-cystine (PubMed:11133847, PubMed:11417227). In addition, mediates the import of L-kynurenine leading to anti-ferroptotic signaling propagation required to maintain L-cystine and glutathione homeostasis (PubMed:35245456). Moreover, mediates N-acetyl-L-cysteine uptake into the placenta leading to subsequently down-regulation of pathways associated with oxidative stress, inflammation and apoptosis (PubMed:34120018). In vitro can also transport L-aspartate (PubMed:11417227). May participate in astrocyte and meningeal cell proliferation during development and can provide neuroprotection by promoting glutathione synthesis and delivery from non-neuronal cells such as astrocytes and meningeal cells to immature neurons (By similarity). Controls the production of pheomelanin pigment directly (By similarity).

Alternative names

Cystine/glutamate transporter, Amino acid transport system xc-, Calcium channel blocker resistance protein CCBR1, Solute carrier family 7 member 11, xCT, SLC7A11

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Clone number

EPR27115-64

Purification technique

Affinity purification Protein A

Specificity

We recommend not to boil your lysates before loading to gel when preparing membrane used for western blot. Boiling leads to the formation of aggregates of membrane proteins, leading to unsuccessful migration in gel. Please do not boil your lysate when testing membrane protein.

FURTHER INFORMATION ON SPECIFICITY (Chinese Version) available under the support & downloads section.

Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Anti-xCT antibody [EPR27115-64] (ab307601) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, IP and WB.

Abcam's high quality manufacturing and validation processes ensure Anti-xCT antibody [EPR27115-64] (ab307601) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-xCT antibody [EPR27115-64] (ab307601) specifically detects xCT (UniProt ID: Q9UPY5; Molecular weight: 56kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).

Conjugation-ready, carrier free format available for antibody clone EPR27115-64 - Anti-xCT antibody [EPR27115-64] - BSA and Azide free ab307602.

A highly validated xCT (SLC7A11) antibody, essential for studying the cystine/glutamate antiporter SLC7A11, ferroptosis and redox balance. This antibody is crucial in cancer research, particularly in understanding xCT's role in tumor progression and antioxidant defense and is widely used in studies of reactive oxygen species (ROS) and xCT inhibitors for therapeutic applications. SLC7A11 is frequently overexpressed in human cancers. SLC7A11 promotes cystine uptake and glutathione biosynthesis, resulting in protection from oxidative stress and ferroptotic cell death. Recent studies have unexpectedly revealed that SLC7A11 also plays critical roles in glutamine metabolism and regulates the glucose and glutamine dependency of cancer cells. Expression of SLC7A11/xCT correlates with advanced lung cancer stage and predicted a worse 5-year survival.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

16 product images

Western blot - Anti-xCT antibody [EPR27115-64] (ab307601)
Western blot: Anti-SLC7A11 antibody [EPR27115-64] (ab307601) staining at 1/1000 dilution, shown in black; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 35 kDa in wild-type HepG2 cell lysates with a reduction in signal observed at this size in SLC7A11 heterozygous knockout cell line. To generate this image, wild-type and SLC7A11 heterozygous knockout HepG2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with a high-sensitivity ECL substrate kit and imaged with 16 minutes exposure time. Secondary antibodies used were Goat anti-Rabbit HRP (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-xCT antibody [EPR27115-64] (ab307601) at 1/1000 dilution
Lane 1: Wild-type HepG2 Vehicle Control Diethyl Maleate (0mM, 24h) cell lysate at 20 µg
Lane 2: Wild-type HepG2 Treated Diethyl Maleate (0.1mM, 24h) cell lysate at 20 µg
Lane 3: SLC7A11 heterozygous knockout HepG2 Vehicle Control Diethyl Maleate (0mM, 24h) cell lysate at 20 µg
Lane 4: SLC7A11 heterozygous knockout HepG2 Treated Diethyl Maleate (0.1mM, 24h) cell lysate at 20 µg
Secondary
All lanes: Goat anti-Rabbit HRP (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 35 kDa
Western blot - Anti-xCT antibody [EPR27115-64] (ab307601)
Western blot: Anti-SLC7A11 antibody [EPR27115-64] (ab307601) staining at 1/1000 dilution, shown in black; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab307601 was shown to bind specifically to SLC7A11. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with a high-sensitivity ECL substrate kit and imaged with 30 seconds exposure time. Secondary antibodies used were Goat anti-Rabbit HRP (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-xCT antibody [EPR27115-64] (ab307601) at 1/1000 dilution
All lanes: Recombinant Human xCT protein cell lysate at 0.01 µg
Secondary
All lanes: Goat anti-Rabbit HRP (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 35 kDa
Western blot - Anti-xCT antibody [EPR27115-64] (ab307601)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: skeletal muscle (PMID:17401668).
The molecular weight observed is consistent with what has been described in the literature (PMID: 26494316, 18806690, 25384799, 30425240, 29693305).

All lysates were not boiled. For getting better result, we recommend not boiling the sample
All lanes: Western blot - Anti-xCT antibody [EPR27115-64] (ab307601) at 1/1000 dilution
Lane 1: Mouse cerebellum tissue lysate at 20 µg
Lane 2: Mouse skeletal muscle tissue lysate at 20 µg
Lane 3: Rat cerebellum tissue lysate at 20 µg
Lane 4: Rat skeletal muscle tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Exposure time: 180s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-xCT antibody [EPR27115-64] (ab307601)
Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue labeling xCT with ab307601 at 1/500 dilution (1.024 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human thyroid carcinoma.
The section was incubated with ab307601 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Western blot - Anti-xCT antibody [EPR27115-64] (ab307601)
Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM/TBST.

Boiling leads to the formation of aggregates of membrane proteins, leading to unsuccessful migration in gel. Please do not boil your lysate when testing membrane protein.
No xCT expression or very low levels were found in lung, heart, liver, and kidney. (PMID: 22667998).
The molecular weight observed is consistent with what has been described in the literature (PMID: 26494316, 18806690, 25384799, 30425240, 29693305).
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a loading control.
All lanes: Western blot - Anti-xCT antibody [EPR27115-64] (ab307601) at 1/1000 dilution
Lanes 1 and 6: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lanes 2 and 7: Mouse cerebellum tissue lysate at 20 µg
Lanes 3 and 8: Mouse colon tissue lysate at 20 µg
Lanes 4 and 9: Mouse kidney tissue lysate at 20 µg
Lanes 10 and 5: Mouse heart tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 37 kDa
Exposure time: 140s
Western blot - Anti-xCT antibody [EPR27115-64] (ab307601)
Blocking and dilution buffer: 5% NFDM/TBST.
xCT protein is easy to form aggregates generating strong signal at the top.
All lanes: Western blot - Anti-xCT antibody [EPR27115-64] (ab307601) at 1/1000 dilution
Lanes 1 and 4: 293T cells transfected with an empty vector containing a His tag whole cell lysate at 20 µg
Lanes 2 and 5: 293T cells transfected with a Human SLC7A5 expression vector containing a His-tag whole cell lysate at 20 µg
Lanes 3 and 6: 293T cells transfected with a Human xCT expression vector containing a His-tag whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 37 kDa
Exposure time: 7s
Western blot - Anti-xCT antibody [EPR27115-64] (ab307601)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Low expression cell line: COLO 205 (PMID: 31000598).

The molecular weight observed is consistent with what has been described in the literature (PMID: 26494316, 18806690, 25384799, 30425240, 29693305).
All lanes: Western blot - Anti-xCT antibody [EPR27115-64] (ab307601) at 1/1000 dilution
Lane 1: Human cerebellum tissue lysate at 20 µg
Lane 2: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: COLO 205 (human colon adenocarcinoma tumor ascitic epithelial cell) whole cell lysate at 20 µg
Lane 4: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 37 kDa
Exposure time: 70s
Immunoprecipitation - Anti-xCT antibody [EPR27115-64] (ab307601)
xCT was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate 10 µg with ab307601 at 1/30 dilution (2 µg in 0.3 5mg lysates). Western blot was performed on the immunoprecipitate using ab307601 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

Lane 1: A549 whole cell lysate 10 µg

Lane 2: ab307601 IP in A549 whole cell lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab307601 in A549 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 41 seconds.
All lanes: Immunoprecipitation - Anti-xCT antibody [EPR27115-64] (ab307601) at 1/30 dilution
All lanes: A549 (human lung carcinoma epithelial cell)
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 37 kDa
Exposure time: 41s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-xCT antibody [EPR27115-64] (ab307601)
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling xCT with ab307601 at 1/500 dilution (1.024 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat skeletal muscle (PMID:17401668).
The section was incubated with ab307601 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-xCT antibody [EPR27115-64] (ab307601)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling xCT with ab307601 at 1/500 dilution (1.024 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse skeletal muscle.
The section was incubated with ab307601 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-xCT antibody [EPR27115-64] (ab307601)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling xCT with ab307601 at 1/500 dilution (1.024 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human skeletal muscle.
The section was incubated with ab307601 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-xCT antibody [EPR27115-64] (ab307601)
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling xCT with ab307601 at 1/500 dilution (1.024 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat cerebrum.
The section was incubated with ab307601 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-xCT antibody [EPR27115-64] (ab307601)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling xCT with ab307601 at 1/500 dilution (1.024 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse cerebrum (PMID:29350434).
The section was incubated with ab307601 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-xCT antibody [EPR27115-64] (ab307601)
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling xCT with ab307601 at 1/500 dilution (1.024 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human cerebrum.
The section was incubated with ab307601 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunocytochemistry/ Immunofluorescence - Anti-xCT antibody [EPR27115-64] (ab307601)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labeling xCT with ab307601 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).
Confocal image showing membranous and cytoplasmic staining in A549 cell line.
Low expression: COLO 205 (PMID: 31000598).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-xCT antibody [EPR27115-64] (ab307601)
Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol-permeabilized COLO 205 (human colon adenocarcinoma tumor ascitic epithelial cell) (Left panel) / A549 (human lung carcinoma epithelial cell) (Right panel) cells labeling xCT with ab307601 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
Low expression: COLO 205 (PMID: 31000598).