Anti-XIAP antibody [EPR22189-113]

• WB

Supplier Data

Western blot - Anti-XIAP antibody [EPR22189-113] (AB229050)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 25535897).

All lanes:

Western blot - Anti-XIAP antibody [EPR22189-113] (ab229050) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

WI-38 (human fetal lung fibroblast cell line) whole cell lysate at 20 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 5:

A549 (human lung carcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 57 kDa

Observed band size: 50-56 kDa

false

Exposure time: 37s

• WB

Lab

Western blot - Anti-XIAP antibody [EPR22189-113] (AB229050)

False colour image of Western blot : Anti-XIAP antibody [EPR22189-113] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab229050 was shown to bind specifically to XIAP. A band was observed at 60 kDa in wild-type A549 cell lysates with no signal observed at this size in XIAP knockout cell line ab289043. To generate this image, wild-type and XIAP knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-XIAP antibody [EPR22189-113] (ab229050) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

XIAP knockout A549 cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

MOLT-4 cell lysate at 20 µg

Observed band size: 60 kDa

false

• WB

Supplier Data

Western blot - Anti-XIAP antibody [EPR22189-113] (AB229050)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-XIAP antibody [EPR22189-113] (ab229050) at 1/1000 dilution

Lane 1:

C6 (rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg

Lane 5:

Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 57 kDa

Observed band size: 50-56 kDa

false

Exposure time: 3min