Anti-XLF antibody [EPR15882-42]

Target data

DNA repair protein involved in DNA non-homologous end joining (NHEJ); required for double-strand break (DSB) repair and V(D)J recombination (PubMed : 16439204, PubMed : 16439205, PubMed : 17317666, PubMed : 17470781, PubMed : 17717001, PubMed : 18158905, PubMed : 18644470, PubMed : 20558749, PubMed : 26100018). Plays a key role in NHEJ by promoting the ligation of various mismatched and non-cohesive ends (PubMed : 17470781, PubMed : 17717001, PubMed : 19056826). Together with PAXX, collaborates with DNA polymerase lambda (POLL) to promote joining of non-cohesive DNA ends (PubMed : 25670504, PubMed : 30250067). May act in concert with XRCC5-XRCC6 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are non-complementary or partially complementary (PubMed : 16439204, PubMed : 16439205, PubMed : 17317666, PubMed : 17470781). In some studies, has been shown to associate with XRCC4 to form alternating helical filaments that bridge DNA and act like a bandage, holding together the broken DNA until it is repaired (PubMed : 21768349, PubMed : 21775435, PubMed : 22228831, PubMed : 22287571, PubMed : 26100018, PubMed : 27437582, PubMed : 28500754). Alternatively, it has also been shown that rather than forming filaments, a single NHEJ1 dimer interacts through both head domains with XRCC4 to promote the close alignment of DNA ends (By similarity). The XRCC4-NHEJ1/XLF subcomplex binds to the DNA fragments of a DSB in a highly diffusive manner and robustly bridges two independent DNA molecules, holding the broken DNA fragments in close proximity to one other (PubMed : 27437582, PubMed : 28500754). The mobility of the bridges ensures that the ends remain accessible for further processing by other repair factors (PubMed : 27437582). Binds DNA in a length-dependent manner (PubMed : 17317666, PubMed : 18158905).