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AB58464

Anti-XPA antibody

2

(1 Review)

|

(2 Publications )

Rabbit Polyclonal XPA antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human XPA.
1 Images
Western blot - Anti-XPA antibody (AB58464)
  • WB

Unknown

Western blot - Anti-XPA antibody (AB58464)

All lanes:

Western blot - Anti-XPA antibody (ab58464) at 1/500 dilution

Lane 1:

Extracts from COLO205 cells

Lane 2:

Extracts from COLO205 cells with immunizing peptide

Predicted band size: 31 kDa

Observed band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human XPA. The exact immunogen used to generate this antibody is proprietary information.

P23025

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The XPA (xeroderma pigmentosum group A) protein also known as DNA repair protein complementing XP-A cells is a vital player in the nucleotide excision repair (NER) machinery. It has a molecular mass of approximately 31 kDa. XPA functions as a DNA-binding protein and is expressed in various tissues including skin cells. XPA recognizes and stabilizes the DNA damage site acting as a scaffold to recruit other repair factors ensuring the proper assembly of essential components in the repair complex.
Biological function summary

The protein is essential for maintaining genomic stability by facilitating the repair of DNA lesions. XPA operates within a multiprotein complex that includes the proteins ERCC1 and XPF. The complex identifies a wide range of structural abnormalities in DNA such as UV-induced photoproducts and chemically induced adducts. Without XPA the NER system's efficiency decreases leaving cells vulnerable to genotoxic stresses causing mutations and damage accumulation.

Pathways

XPA plays a role in the nucleotide excision repair and the base excision repair pathways. These pathways work jointly to ensure the removal of damaged DNA sections and accurate DNA synthesis. Within the NER pathway XPA interacts with proteins like RPA (Replication Protein A) and TFIIH (Transcription Factor IIH) to mediate the excision of damaged oligonucleotides. Its activity is critical for preserving the integrity of the genetic code especially in response to DNA damage caused by external agents like UV radiation.

Mutations or deficiencies in XPA are linked to xeroderma pigmentosum a disorder characterized by extreme sensitivity to ultraviolet light and a heightened risk of skin cancer. This condition results from impaired DNA repair leading to the accumulation of unrepaired DNA damage. XPA also interacts with other NER proteins like ERCC1 and XPF whose dysfunction is implicated in similar DNA repair disorders emphasizing the interconnected nature of these repair pathways in protecting against genotoxic agents.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in DNA nucleotide excision repair (NER). Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation (PubMed : 19197159). During NER stimulates the 5'-3' helicase activity of XPD/ERCC2 and the DNA translocase activity of XPB/ERCC3 (PubMed : 31253769). Connects XPD/ERCC2 and XPB/ERCC3 during NER, retaining DNA near the XPB/ERCC3 active site, and stabilizing the complex in a different conformation than in transcribing TFIIH (PubMed : 31253769).
See full target information XPA

Alternative Names

XPAC, XPA, DNA repair protein complementing XP-A cells, Xeroderma pigmentosum group A-complementing protein
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 13:880413 PubMed35634291

2022

Genome Replication Is Associated With Release of Immunogenic DNA Waste.

Applications

Unspecified application

Species

Unspecified reactive species

Nadja Schubert,Tina Schumann,Elena Daum,Karolin Flade,Yan Ge,Lara Hagedorn,Winfried Edelmann,Luise Müller,Marc Schmitz,Gunnar Kuut,Veit Hornung,Rayk Behrendt,Axel Roers

BMC cancer 21:265 PubMed33706745

2021

Secondary gliosarcoma: the clinicopathological features and the development of a patient-derived xenograft model of gliosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Karrie Mei-Yee Kiang,Andrian A Chan,Gilberto Ka-Kit Leung
View all publications
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Product promise

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