Rabbit Polyclonal XPA antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human XPA aa 1-50.
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- Cell death & disease 13:10342022Idarubicin combats abiraterone and enzalutamide resistance in prostate cells via targeting XPA protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Zhang,Wei Wei,Changying Li,Siyuan Yan,Shanshan Wang,Shudong Xiao,Chenchen He,Jing Li,Zhi Qi,Benyi Li,Kuo Yang,Changlin LiPubMed 36509750
- Frontiers in genetics 13:10094302022Microtubule modification defects underlie cilium degeneration in cell models of retinitis pigmentosa associated with pre-mRNA splicing factor mutations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiliya Nazlamova,Suly Saray Villa Vasquez,Jenny Lord,Varshini Karthik,Man-Kim Cheung,Jörn Lakowski,Gabrielle WhewayPubMed 36176300
- Nature communications 11:2362020Distinct DNA repair pathways cause genomic instability at alternative DNA structures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJennifer A McKinney et. al.PubMed 31932649
- Nature communications 9:2592018The protective role of DOT1L in UV-induced melanomagenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Zhu et. al.PubMed 29343685
- Toxicology and applied pharmacology 331:108-1152017Differential sensitivities of cellular XPA and PARP-1 to arsenite inhibition and zinc rescue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaofeng Ding et. al.PubMed 28552776
- Chemical research in toxicology 27:690-82014Differential binding of monomethylarsonous acid compared to arsenite and arsenic trioxide with zinc finger peptides and proteins.
- The Journal of biological chemistry 287:39824-332012Poly(ADP-ribose) contributes to an association between poly(ADP-ribose) polymerase-1 and xeroderma pigmentosum complementation group A in nucleotide excision repair.
- The Journal of biological chemistry 286:22855-632011Arsenite interacts selectively with zinc finger proteins containing C3H1 or C4 motifs.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human XPA aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P23025
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Elephant | Predicted | Predicted | Predicted |
| Gorilla | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey, Gorilla, Elephant | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey, Gorilla, Elephant | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey, Gorilla, Elephant | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Involved in DNA nucleotide excision repair (NER). Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation (PubMed:19197159). During NER stimulates the 5'-3' helicase activity of XPD/ERCC2 and the DNA translocase activity of XPB/ERCC3 (PubMed:31253769). Connects XPD/ERCC2 and XPB/ERCC3 during NER, retaining DNA near the XPB/ERCC3 active site, and stabilizing the complex in a different conformation than in transcribing TFIIH (PubMed:31253769).
Alternative names
XPAC, XPA, DNA repair protein complementing XP-A cells, Xeroderma pigmentosum group A-complementing protein
Recommended products
Rabbit Polyclonal XPA antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human XPA aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human XPA aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P23025
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab85914 was affinity purified using an epitope specific to XPA immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The XPA (xeroderma pigmentosum group A) protein also known as DNA repair protein complementing XP-A cells is a vital player in the nucleotide excision repair (NER) machinery. It has a molecular mass of approximately 31 kDa. XPA functions as a DNA-binding protein and is expressed in various tissues including skin cells. XPA recognizes and stabilizes the DNA damage site acting as a scaffold to recruit other repair factors ensuring the proper assembly of essential components in the repair complex.
- Biological function summary
The protein is essential for maintaining genomic stability by facilitating the repair of DNA lesions. XPA operates within a multiprotein complex that includes the proteins ERCC1 and XPF. The complex identifies a wide range of structural abnormalities in DNA such as UV-induced photoproducts and chemically induced adducts. Without XPA the NER system's efficiency decreases leaving cells vulnerable to genotoxic stresses causing mutations and damage accumulation.
- Pathways
XPA plays a role in the nucleotide excision repair and the base excision repair pathways. These pathways work jointly to ensure the removal of damaged DNA sections and accurate DNA synthesis. Within the NER pathway XPA interacts with proteins like RPA (Replication Protein A) and TFIIH (Transcription Factor IIH) to mediate the excision of damaged oligonucleotides. Its activity is critical for preserving the integrity of the genetic code especially in response to DNA damage caused by external agents like UV radiation.
- Associated diseases and disorders
Mutations or deficiencies in XPA are linked to xeroderma pigmentosum a disorder characterized by extreme sensitivity to ultraviolet light and a heightened risk of skin cancer. This condition results from impaired DNA repair leading to the accumulation of unrepaired DNA damage. XPA also interacts with other NER proteins like ERCC1 and XPF whose dysfunction is implicated in similar DNA repair disorders emphasizing the interconnected nature of these repair pathways in protecting against genotoxic agents.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-XPA antibody (ab85914)
Detection: Chemiluminescence with exposure time of 30 seconds
All lanes: Western blot - Anti-XPA antibody (ab85914) at 0.04 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Predicted band size: 31 kDa
Observed band size: 39 kDa, 55 kDa
Exposure time: 30s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPA antibody (ab85914)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling XPA with ab85914 at 1/1000 (0.2µg/ml). Detection: DAB.
Immunoprecipitation - Anti-XPA antibody (ab85914)
Detection of XPA by Western Blot of Immunprecipitate.
ab85914 at 0.1µg/ml staining XPA in HeLa whole cell lysate immunoprecipitated using ab85914 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.All lanes: Immunoprecipitation - Anti-XPA antibody (ab85914)
Predicted band size: 31 kDa
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