JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB85914

Anti-XPA antibody

4

(1 Review)

|

(10 Publications)

Rabbit Polyclonal XPA antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human XPA aa 1-50.

View Alternative Names

XPAC, XPA, DNA repair protein complementing XP-A cells, Xeroderma pigmentosum group A-complementing protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPA antibody (AB85914)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPA antibody (AB85914)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling XPA with ab85914 at 1/1000 (0.2µg/ml). Detection : DAB.

Immunoprecipitation - Anti-XPA antibody (AB85914)
  • IP

Unknown

Immunoprecipitation - Anti-XPA antibody (AB85914)

Detection of XPA by Western Blot of Immunprecipitate.
ab85914 at 0.1µg/ml staining XPA in HeLa whole cell lysate immunoprecipitated using ab85914 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-XPA antibody (ab85914)

Predicted band size: 31 kDa

false

Western blot - Anti-XPA antibody (AB85914)
  • WB

Unknown

Western blot - Anti-XPA antibody (AB85914)

Detection : Chemiluminescence with exposure time of 30 seconds

All lanes:

Western blot - Anti-XPA antibody (ab85914) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 31 kDa

Observed band size: 39 kDa,55 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP, IHC-P

applications

Immunogen

Synthetic Peptide within Human XPA aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P23025

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Elephant": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab85914 was affinity purified using an epitope specific to XPA immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The XPA (xeroderma pigmentosum group A) protein also known as DNA repair protein complementing XP-A cells is a vital player in the nucleotide excision repair (NER) machinery. It has a molecular mass of approximately 31 kDa. XPA functions as a DNA-binding protein and is expressed in various tissues including skin cells. XPA recognizes and stabilizes the DNA damage site acting as a scaffold to recruit other repair factors ensuring the proper assembly of essential components in the repair complex.
Biological function summary

The protein is essential for maintaining genomic stability by facilitating the repair of DNA lesions. XPA operates within a multiprotein complex that includes the proteins ERCC1 and XPF. The complex identifies a wide range of structural abnormalities in DNA such as UV-induced photoproducts and chemically induced adducts. Without XPA the NER system's efficiency decreases leaving cells vulnerable to genotoxic stresses causing mutations and damage accumulation.

Pathways

XPA plays a role in the nucleotide excision repair and the base excision repair pathways. These pathways work jointly to ensure the removal of damaged DNA sections and accurate DNA synthesis. Within the NER pathway XPA interacts with proteins like RPA (Replication Protein A) and TFIIH (Transcription Factor IIH) to mediate the excision of damaged oligonucleotides. Its activity is critical for preserving the integrity of the genetic code especially in response to DNA damage caused by external agents like UV radiation.

Mutations or deficiencies in XPA are linked to xeroderma pigmentosum a disorder characterized by extreme sensitivity to ultraviolet light and a heightened risk of skin cancer. This condition results from impaired DNA repair leading to the accumulation of unrepaired DNA damage. XPA also interacts with other NER proteins like ERCC1 and XPF whose dysfunction is implicated in similar DNA repair disorders emphasizing the interconnected nature of these repair pathways in protecting against genotoxic agents.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in DNA nucleotide excision repair (NER). Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation (PubMed : 19197159). During NER stimulates the 5'-3' helicase activity of XPD/ERCC2 and the DNA translocase activity of XPB/ERCC3 (PubMed : 31253769). Connects XPD/ERCC2 and XPB/ERCC3 during NER, retaining DNA near the XPB/ERCC3 active site, and stabilizing the complex in a different conformation than in transcribing TFIIH (PubMed : 31253769).
See full target information XPA
style
left-column

Publications (10)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40244040

2025

Transcription Factor p73 Is a Predictor of Platinum Resistance and Promotes Aggressive Epithelial Ovarian Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Shoqafi,Reem Ali,Ayat Lashen,Jennie N Jeyapalan,Asmaa Ibrahim,Michael S Toss,Emad A Rakha,Mashael Algethami,Shatha Alqahtani,Nigel P Mongan,Dindial Ramotar,Srinivasan Madhusudan

NAR cancer 6:zcae013 PubMed38500596

2024

XPA tumor variant leads to defects in NER that sensitize cells to cisplatin.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra M Blee,Kaitlyn S Gallagher,Hyun-Suk Kim,Mihyun Kim,Suhas S Kharat,Christina R Troll,Areetha D'Souza,Jiyoung Park,P Drew Neufer,Orlando D Schärer,Walter J Chazin

Cell death & disease 13:1034 PubMed36509750

2022

Idarubicin combats abiraterone and enzalutamide resistance in prostate cells via targeting XPA protein.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhang,Wei Wei,Changying Li,Siyuan Yan,Shanshan Wang,Shudong Xiao,Chenchen He,Jing Li,Zhi Qi,Benyi Li,Kuo Yang,Changlin Li

Frontiers in genetics 13:1009430 PubMed36176300

2022

Microtubule modification defects underlie cilium degeneration in cell models of retinitis pigmentosa associated with pre-mRNA splicing factor mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Liliya Nazlamova,Suly Saray Villa Vasquez,Jenny Lord,Varshini Karthik,Man-Kim Cheung,Jörn Lakowski,Gabrielle Wheway

Nature communications 11:236 PubMed31932649

2020

Distinct DNA repair pathways cause genomic instability at alternative DNA structures.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer A McKinney,Guliang Wang,Anirban Mukherjee,Laura Christensen,Sai H Sankara Subramanian,Junhua Zhao,Karen M Vasquez

Nature communications 9:259 PubMed29343685

2018

The protective role of DOT1L in UV-induced melanomagenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Zhu,Shuyang Chen,Hongshen Wang,Chengqian Yin,Changpeng Han,Cong Peng,Zhaoqian Liu,Lixin Wan,Xiaoyang Zhang,Jie Zhang,Christine G Lian,Peilin Ma,Zhi-Xiang Xu,Sharon Prince,Tao Wang,Xiumei Gao,Yujiang Shi,Dali Liu,Min Liu,Wenyi Wei,Zhi Wei,Jingxuan Pan,Yongjun Wang,Zhenyu Xuan,Jay Hess,Nicholas K Hayward,Colin R Goding,Xiang Chen,Jun Zhou,Rutao Cui

Toxicology and applied pharmacology 331:108-115 PubMed28552776

2017

Differential sensitivities of cellular XPA and PARP-1 to arsenite inhibition and zinc rescue.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofeng Ding,Xixi Zhou,Karen L Cooper,Juliana Huestis,Laurie G Hudson,Ke Jian Liu

Chemical research in toxicology 27:690-8 PubMed24611629

2014

Differential binding of monomethylarsonous acid compared to arsenite and arsenic trioxide with zinc finger peptides and proteins.

Applications

IP

Species

Human

Xixi Zhou,Xi Sun,Charlotte Mobarak,A Jay Gandolfi,Scott W Burchiel,Laurie G Hudson,Ke Jian Liu

The Journal of biological chemistry 287:39824-33 PubMed23038248

2012

Poly(ADP-ribose) contributes to an association between poly(ADP-ribose) polymerase-1 and xeroderma pigmentosum complementation group A in nucleotide excision repair.

Applications

IP

Species

Human

Brenee S King,Karen L Cooper,Ke Jian Liu,Laurie G Hudson

The Journal of biological chemistry 286:22855-63 PubMed21550982

2011

Arsenite interacts selectively with zinc finger proteins containing C3H1 or C4 motifs.

Applications

IP

Species

Human

Xixi Zhou,Xi Sun,Karen L Cooper,Feng Wang,Ke Jian Liu,Laurie G Hudson
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB58464

Anti-XPA antibody

Western blot - Anti-XPA antibody (AB58464)

  • 2
  • 1
Primary Antibodies

AB65963

Anti-XPA antibody [5F12]

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-XPA antibody [5F12] (AB65963)

  • 4
  • 1
Primary Antibodies

AB180618

Anti-XPA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPA antibody (AB180618)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB290740

Anti-XPF antibody [EPR25350-148] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-XPF antibody [EPR25350-148] - BSA and Azide free (AB290740)

  • 0
  • 0
Primary Antibodies

AB6326

Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

Flow Cytometry (Intracellular) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (AB6326)

  • 5
  • 104
Primary Antibodies

AB79398

Anti-RPA70 antibody [EPR3472]

RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-RPA70 antibody [EPR3472] (AB79398)

  • 5
  • 6
Primary Antibodies

AB76420

Anti-RPA32/RPA2 antibody [EPR2877Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2877Y] (AB76420)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com