AB245538

Anti-XPB antibody

Name: Anti-XPB antibody (ab245538) | Abcam
Brand: Abcam Limited
SKU: AB245538

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(1 Publication)

Rabbit Polyclonal XPB antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ERCC3 aa 700 to C-terminus.

View Alternative Names

XPB, XPBC, ERCC3, General transcription and DNA repair factor IIH helicase/translocase subunit XPB, TFIIH subunit XPB, Basic transcription factor 2 89 kDa subunit, DNA 3'-5' helicase/translocase XPB, DNA excision repair protein ERCC-3, DNA repair protein complementing XP-B cells, TFIIH basal transcription factor complex 89 kDa subunit, Xeroderma pigmentosum group B-complementing protein, BTF2 p89, TFIIH 89 kDa subunit, TFIIH p89

2 Images

Supplier Data

Immunoprecipitation - Anti-XPB antibody (AB245538)

XPB was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245538 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab245538 at 1 μg/ml.

Lane 1 : ab245538 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-XPB antibody (ab245538)

Predicted band size: 89 kDa

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Supplier Data

Western blot - Anti-XPB antibody (AB245538)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-XPB antibody (ab245538) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 15 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 15 µg

Predicted band size: 89 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human ERCC3 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P19447

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab245538 was affinity purified using an epitope specific to XPB immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

XPB also known as ERCC3 is part of the helicase family. It has an approximate mass of 89 kDa. XPB uses ATP to unwind DNA which facilitates various genetic processes. In humans it expresses in tissues where there is active DNA replication and repair occurring including rapidly dividing cells. Its activity is important to the transcription process and DNA damage repair mechanism.

Biological function summary

XPB assists the cellular response to DNA damage by being part of the TFIIH complex which is involved in transcription. This complex plays a role in nucleotide excision repair (NER) where it unwinds DNA segments to allow for lesion repair. In conjunction with other proteins XPB facilitates the resolution of DNA loops and manages the clearance of polymerase during transcription-coupled repair ensuring proper DNA repair and transcription restart.

Pathways

XPB influences both transcription initiation and DNA damage repair being integral to the mechanisms that maintain genome integrity. It interacts closely with proteins like XPD within the NER pathway and its roles in these pathways contribute to the prevention of mutations. XPB's function as a helicase denotes its participation in opening DNA for repair or transcription therefore supporting cell viability and genomic stability.

XPB's deficiency or malfunction links to xeroderma pigmentosum and trichothiodystrophy. These conditions stem from impaired nucleotide excision repair where mutations in XPB affect the efficiency of DNA repair and proper cellular response to damage. Symptoms correlated with these diseases often include increased sensitivity to UV light and neurological deficits connected through pathways involving other TFIIH complex components like XPD signifying the collaboration needed within a cell for effective DNA repair.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ATP-dependent 3'-5' DNA helicase/translocase (PubMed : 17466626, PubMed : 27193682, PubMed : 33902107, PubMed : 8465201, PubMed : 8663148). Binds dsDNA rather than ssDNA, unzipping it in a translocase rather than classical helicase activity (PubMed : 27193682, PubMed : 33902107). Component of the general transcription and DNA repair factor IIH (TFIIH) core complex (PubMed : 10024882, PubMed : 17466626, PubMed : 8157004, PubMed : 8465201). When complexed to CDK-activating kinase (CAK), involved in RNA transcription by RNA polymerase II. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening; it may wrap around the damaged DNA wedging it open, causing localized melting that allows XPD/ERCC2 helicase to anchor (PubMed : 10024882, PubMed : 17466626). In transcription, TFIIH has an essential role in transcription initiation (PubMed : 30894545, PubMed : 8157004). When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape (PubMed : 8157004). The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape (PubMed : 10024882). In transcription pre-initiation complexes induces and propagates a DNA twist to open DNA (PubMed : 27193682, PubMed : 33902107). Also involved in transcription-coupled nucleotide excision repair (NER) of damaged DNA (PubMed : 17466626, PubMed : 2111438, PubMed : 8157004). In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The structure of the TFIIH transcription complex differs from the NER-TFIIH complex; large movements by XPD/ERCC2 and XPB/ERCC3 are stabilized by XPA (PubMed : 31253769, PubMed : 33902107). XPA retains XPB/ERCC3 at the 5' end of a DNA bubble (mimicking DNA damage) (PubMed : 31253769).

See full target information ERCC3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

NPJ precision oncology 7:28 PubMed36922568

2023

The SMARCA4 mutation facilitates chromatin remodeling and confers PRMT1/SMARCA4 inhibitors sensitivity in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangwei Zeng,Bing Yao,Jianpeng Liu,Guan-Wen Gong,Ming Liu,Jiahuang Li,Hua-Feng Pan,Qixiang Li,Dongjun Yang,Peifen Lu,Dongliang Wu,Peipei Xu,Bing Chen,Panhai Chen,Ming Zhang,Ke Zen,Jian Jing,David C S Huang,Dijun Chen,Zhi-Wei Jiang,Quan Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-XPB antibody

Immunoprecipitation - Anti-XPB antibody (AB245538)

Western blot - Anti-XPB antibody (AB245538)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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