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AB190698

Anti-XPB antibody [EPR14161]

5

(1 Review)

|

(5 Publications)

Rabbit Recombinant Monoclonal XPB antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications.

View Alternative Names

XPB, XPBC, ERCC3, General transcription and DNA repair factor IIH helicase/translocase subunit XPB, TFIIH subunit XPB, Basic transcription factor 2 89 kDa subunit, DNA 3'-5' helicase/translocase XPB, DNA excision repair protein ERCC-3, DNA repair protein complementing XP-B cells, TFIIH basal transcription factor complex 89 kDa subunit, Xeroderma pigmentosum group B-complementing protein, BTF2 p89, TFIIH 89 kDa subunit, TFIIH p89

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-XPB antibody [EPR14161] (AB190698)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-XPB antibody [EPR14161] (AB190698)

Immunocytochemical analysis of 4% Paraformaldehyde fixed A549 cells, labeling XPB using ab190698 at a 1/500 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at a 1/200 dilutiion and counter-stained with Dapi.

Western blot - Anti-XPB antibody [EPR14161] (AB190698)
  • WB

Supplier Data

Western blot - Anti-XPB antibody [EPR14161] (AB190698)

All lanes:

Western blot - Anti-XPB antibody [EPR14161] (ab190698) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 89 kDa

false

  • Carrier free

    Anti-XPB antibody [EPR14161] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14161

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

XPB also known as ERCC3 is part of the helicase family. It has an approximate mass of 89 kDa. XPB uses ATP to unwind DNA which facilitates various genetic processes. In humans it expresses in tissues where there is active DNA replication and repair occurring including rapidly dividing cells. Its activity is important to the transcription process and DNA damage repair mechanism.
Biological function summary

XPB assists the cellular response to DNA damage by being part of the TFIIH complex which is involved in transcription. This complex plays a role in nucleotide excision repair (NER) where it unwinds DNA segments to allow for lesion repair. In conjunction with other proteins XPB facilitates the resolution of DNA loops and manages the clearance of polymerase during transcription-coupled repair ensuring proper DNA repair and transcription restart.

Pathways

XPB influences both transcription initiation and DNA damage repair being integral to the mechanisms that maintain genome integrity. It interacts closely with proteins like XPD within the NER pathway and its roles in these pathways contribute to the prevention of mutations. XPB's function as a helicase denotes its participation in opening DNA for repair or transcription therefore supporting cell viability and genomic stability.

XPB's deficiency or malfunction links to xeroderma pigmentosum and trichothiodystrophy. These conditions stem from impaired nucleotide excision repair where mutations in XPB affect the efficiency of DNA repair and proper cellular response to damage. Symptoms correlated with these diseases often include increased sensitivity to UV light and neurological deficits connected through pathways involving other TFIIH complex components like XPD signifying the collaboration needed within a cell for effective DNA repair.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP-dependent 3'-5' DNA helicase/translocase (PubMed : 17466626, PubMed : 27193682, PubMed : 33902107, PubMed : 8465201, PubMed : 8663148). Binds dsDNA rather than ssDNA, unzipping it in a translocase rather than classical helicase activity (PubMed : 27193682, PubMed : 33902107). Component of the general transcription and DNA repair factor IIH (TFIIH) core complex (PubMed : 10024882, PubMed : 17466626, PubMed : 8157004, PubMed : 8465201). When complexed to CDK-activating kinase (CAK), involved in RNA transcription by RNA polymerase II. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening; it may wrap around the damaged DNA wedging it open, causing localized melting that allows XPD/ERCC2 helicase to anchor (PubMed : 10024882, PubMed : 17466626). In transcription, TFIIH has an essential role in transcription initiation (PubMed : 30894545, PubMed : 8157004). When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape (PubMed : 8157004). The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape (PubMed : 10024882). In transcription pre-initiation complexes induces and propagates a DNA twist to open DNA (PubMed : 27193682, PubMed : 33902107). Also involved in transcription-coupled nucleotide excision repair (NER) of damaged DNA (PubMed : 17466626, PubMed : 2111438, PubMed : 8157004). In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The structure of the TFIIH transcription complex differs from the NER-TFIIH complex; large movements by XPD/ERCC2 and XPB/ERCC3 are stabilized by XPA (PubMed : 31253769, PubMed : 33902107). XPA retains XPB/ERCC3 at the 5' end of a DNA bubble (mimicking DNA damage) (PubMed : 31253769).
See full target information ERCC3
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Medical gas research 15:459-470 PubMed40300881

2025

Magnesium-assisted hydrogen improves isoproterenol-induced heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Fengbao Chen,Ruimin Chen,Lili Yang,Bowen Shen,Yunting Wang,Yongfeng Gao,Rui Tan,Xiaomin Zhao

Nature cell biology 26:770-783 PubMed38600236

2024

Transcription-coupled DNA-protein crosslink repair by CSB and CRL4-mediated degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Marjolein van Sluis,Qing Yu,Melanie van der Woude,Camila Gonzalo-Hansen,Shannon C Dealy,Roel C Janssens,Hedda B Somsen,Anisha R Ramadhin,Dick H W Dekkers,Hannah Lena Wienecke,Joris J P G Demmers,Anja Raams,Carlota Davó-Martínez,Diana A Llerena Schiffmacher,Marvin van Toorn,David Häckes,Karen L Thijssen,Di Zhou,Judith G Lammers,Alex Pines,Wim Vermeulen,Joris Pothof,Jeroen A A Demmers,Debbie L C van den Berg,Hannes Lans,Jurgen A Marteijn

Cell reports methods 4:100674 PubMed38176411

2024

Live-cell imaging of endogenous CSB-mScarletI as a sensitive marker for DNA-damage-induced transcription stress.

Applications

Unspecified application

Species

Unspecified reactive species

Di Zhou,Qing Yu,Roel C Janssens,Jurgen A Marteijn

The EMBO journal 42:e110937 PubMed36382717

2022

Anti-hsa-miR-59 alleviates premature senescence associated with Hutchinson-Gilford progeria syndrome in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qianying Hu,Na Zhang,Tingting Sui,Guanlin Li,Zhiyao Wang,Mingyue Liu,Xiaojuan Zhu,Baiqu Huang,Jun Lu,Zhanjun Li,Yu Zhang

Journal of Cancer 12:2550-2559 PubMed33854616

2021

Overexpression of ERCC3 is associated with poor prognosis in patients with pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shujie Wang,Wenjing Liu,Yueli Ni,Lifeng Wang,Yuzhi Zhu,Qiong Shi,Zihan Yi,Wenjie Wang,Lili Liu,Lijuan Yang,Yingmin Kuang,Yuechun Zhu,Qiao Zhang,Zhe Yang
View all publications
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Product promise

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