JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB236128

Anti-XPB antibody [EPR14161] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal XPB antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

XPB, XPBC, ERCC3, General transcription and DNA repair factor IIH helicase/translocase subunit XPB, TFIIH subunit XPB, Basic transcription factor 2 89 kDa subunit, DNA 3'-5' helicase/translocase XPB, DNA excision repair protein ERCC-3, DNA repair protein complementing XP-B cells, TFIIH basal transcription factor complex 89 kDa subunit, Xeroderma pigmentosum group B-complementing protein, BTF2 p89, TFIIH 89 kDa subunit, TFIIH p89

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-XPB antibody [EPR14161] - BSA and Azide free (AB236128)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-XPB antibody [EPR14161] - BSA and Azide free (AB236128)

Immunocytochemical analysis of 4% Paraformaldehyde fixed A549 cells, labeling XPB using ab190698 at a 1/500 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at a 1/200 dilutiion and counter-stained with Dapi.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190698).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14161

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB245538

Anti-XPB antibody

Immunoprecipitation - Anti-XPB antibody (AB245538)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB6326

Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

Flow Cytometry (Intracellular) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (AB6326)

  • 5
  • 104
Primary Antibodies

AB309129

Anti-XPC antibody [EPR25076-9]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPC antibody [EPR25076-9] (AB309129)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB16048

Anti-Lamin B1 antibody - Nuclear Envelope Marker

BOND RX™ Validated|KO Validated|Lab Essentials

Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (AB16048)

  • 5
  • 110
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

ab236128 is the carrier-free version of ab190698.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

XPB also known as ERCC3 is part of the helicase family. It has an approximate mass of 89 kDa. XPB uses ATP to unwind DNA which facilitates various genetic processes. In humans it expresses in tissues where there is active DNA replication and repair occurring including rapidly dividing cells. Its activity is important to the transcription process and DNA damage repair mechanism.
Biological function summary

XPB assists the cellular response to DNA damage by being part of the TFIIH complex which is involved in transcription. This complex plays a role in nucleotide excision repair (NER) where it unwinds DNA segments to allow for lesion repair. In conjunction with other proteins XPB facilitates the resolution of DNA loops and manages the clearance of polymerase during transcription-coupled repair ensuring proper DNA repair and transcription restart.

Pathways

XPB influences both transcription initiation and DNA damage repair being integral to the mechanisms that maintain genome integrity. It interacts closely with proteins like XPD within the NER pathway and its roles in these pathways contribute to the prevention of mutations. XPB's function as a helicase denotes its participation in opening DNA for repair or transcription therefore supporting cell viability and genomic stability.

XPB's deficiency or malfunction links to xeroderma pigmentosum and trichothiodystrophy. These conditions stem from impaired nucleotide excision repair where mutations in XPB affect the efficiency of DNA repair and proper cellular response to damage. Symptoms correlated with these diseases often include increased sensitivity to UV light and neurological deficits connected through pathways involving other TFIIH complex components like XPD signifying the collaboration needed within a cell for effective DNA repair.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

ATP-dependent 3'-5' DNA helicase/translocase (PubMed : 17466626, PubMed : 27193682, PubMed : 33902107, PubMed : 8465201, PubMed : 8663148). Binds dsDNA rather than ssDNA, unzipping it in a translocase rather than classical helicase activity (PubMed : 27193682, PubMed : 33902107). Component of the general transcription and DNA repair factor IIH (TFIIH) core complex (PubMed : 10024882, PubMed : 17466626, PubMed : 8157004, PubMed : 8465201). When complexed to CDK-activating kinase (CAK), involved in RNA transcription by RNA polymerase II. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening; it may wrap around the damaged DNA wedging it open, causing localized melting that allows XPD/ERCC2 helicase to anchor (PubMed : 10024882, PubMed : 17466626). In transcription, TFIIH has an essential role in transcription initiation (PubMed : 30894545, PubMed : 8157004). When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape (PubMed : 8157004). The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape (PubMed : 10024882). In transcription pre-initiation complexes induces and propagates a DNA twist to open DNA (PubMed : 27193682, PubMed : 33902107). Also involved in transcription-coupled nucleotide excision repair (NER) of damaged DNA (PubMed : 17466626, PubMed : 2111438, PubMed : 8157004). In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The structure of the TFIIH transcription complex differs from the NER-TFIIH complex; large movements by XPD/ERCC2 and XPB/ERCC3 are stabilized by XPA (PubMed : 31253769, PubMed : 33902107). XPA retains XPB/ERCC3 at the 5' end of a DNA bubble (mimicking DNA damage) (PubMed : 31253769).
See full target information ERCC3
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurobiology 60:672-686 PubMed36357613

2022

Mito-TEMPO, a Mitochondria-Targeted Antioxidant, Improves Cognitive Dysfunction due to Hypoglycemia: an Association with Reduced Pericyte Loss and Blood-Brain Barrier Leakage.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Lin,Zhou Chen,Cuihua Huang,Yubin Wu,Lishan Huang,Lijing Wang,Sujie Ke,Libin Liu
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com