Anti-XPC antibody [EPR25076-9] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPC antibody [EPR25076-9] - BSA and Azide free (AB309130)

This data was developed using ab309129, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling XPC with ab309129 at 1/100 (5.02 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human testis. The section was incubated with ab309129 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPC antibody [EPR25076-9] - BSA and Azide free (AB309130)

This data was developed using ab309129, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human cervical carci tissue labeling XPC with ab309129 at 1/100 (5.02 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cervical carcinoma. The section was incubated with ab309129 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPC antibody [EPR25076-9] - BSA and Azide free (AB309130)

This data was developed using ab309129, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling XPC with ab309129 at 1/100 (5.02 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human skin (PMID : 20616346). The section was incubated with ab309129 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-XPC antibody [EPR25076-9] - BSA and Azide free (AB309130)

This data was developed using ab309129, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST The identity of the higher Mw band in lanes 2 & 3 is unknown. The blot of lanes 2 & 3 was developed using a higher sensitivity ECL substrate. Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : Lane1 : 180 seconds, Lane2-3 : 114 seconds

All lanes:

Western blot - Anti-XPC antibody [EPR25076-9] (<a href='/en-us/products/primary-antibodies/xpc-antibody-epr25076-9-ab309129'>ab309129</a>) at 1/1000 dilution

Lane 1:

MCF7(human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 3:

HeLa transfected with siRNA specifically targeti XPC whole cell lysate 20 at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 125 kDa

false

• WB

Supplier Data

Western blot - Anti-XPC antibody [EPR25076-9] - BSA and Azide free (AB309130)

This data was developed using ab309129, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST The identity of the higher Mw band in lanes 1 & 2 is unknown. Lanes1-2 were freshly made and used for Western blotting immediately to minimize protein degradation. This blot was developed using a high sensitivity ECL substrate. Exposure time : 180 seconds

All lanes:

Western blot - Anti-XPC antibody [EPR25076-9] (<a href='/en-us/products/primary-antibodies/xpc-antibody-epr25076-9-ab309129'>ab309129</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

Raji (human burkitts lymphoma b lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 125 kDa

false

Exposure time: 180s