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AB111596

Anti-XPD antibody

5

(1 Review)

|

(2 Publications)

Rabbit Polyclonal XPD antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ERCC2 aa 1-400.

View Alternative Names

XPD, XPDC, ERCC2, General transcription and DNA repair factor IIH helicase subunit XPD, TFIIH subunit XPD, Basic transcription factor 2 80 kDa subunit, CXPD, DNA 5'-3' helicase XPD, DNA excision repair protein ERCC-2, DNA repair protein complementing XP-D cells, TFIIH basal transcription factor complex 80 kDa subunit, Xeroderma pigmentosum group D-complementing protein, BTF2 p80, TFIIH 80 kDa subunit, TFIIH p80

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody (AB111596)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody (AB111596)

ab111596 at range of 1/100- 1/1000 dilution staining XPD in paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-XPD antibody (AB111596)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-XPD antibody (AB111596)

ab111596 at 1/500 dilution staining XPD in Paraformaldehyde-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.

Western blot - Anti-XPD antibody (AB111596)
  • WB

Unknown

Western blot - Anti-XPD antibody (AB111596)

7.5% SDS-PAGE.

All lanes:

Western blot - Anti-XPD antibody (ab111596) at 1/1000 dilution

All lanes:

MCF7 whole cell lysate at 30 µg

Predicted band size: 87 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ERCC2 aa 1-400. The exact immunogen used to generate this antibody is proprietary information.

P18074

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Alternative antigen retrieval method: Tris-EDTA buffer pH 8.0</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab111596 is purified by antigen affinity chromatography.
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

XPD also known as ERCC2 is an essential helicase enzyme belonging to the family of ATP-dependent DNA helicases. It consists of approximately 761 amino acids and has a mass of around 87 kDa. XPD functions mechanically by unwinding double-stranded DNA which is important for various DNA repair processes. This protein is expressed ubiquitously in human tissues highlighting its critical role in maintaining genomic integrity.
Biological function summary

XPD catalyzes the unwinding of DNA within the transcription factor IIH (TFIIH) complex. This complex facilitates DNA repair and transcription initiation. XPD participates in the nucleotide excision repair (NER) pathway where it contributes to repairing damaged DNA by excising damaged nucleotides. Its role in transcription-coupled repair and general transcription initiation underlines its importance in cellular homeostasis.

Pathways

DNA repair and general transcription processes are significantly affected by XPD. It plays an integral role in nucleotide excision repair (NER) and transcription. Within these pathways XPD works closely with other proteins such as XPA which assists in damage verification and incision near DNA lesions. XPD's unwinding function is essential for the correct orientation of DNA during transcription and repair events ensuring accurate gene expression and stability.

XPD mutations link to xeroderma pigmentosum (XP) and trichothiodystrophy (TTD). These conditions often result in increased sensitivity to ultraviolet (UV) radiation and various developmental abnormalities. XPD mutations impair the repair of UV-induced DNA damage causing symptoms in both XP and TTD. XPD also connects with ERCC1 a protein important for repair synthesis and DNA incision highlighting its broad impact on human health when its function is compromised.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP-dependent 5'-3' DNA helicase (PubMed : 31253769, PubMed : 8413672, PubMed : 9771713). Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, not absolutely essential for minimal transcription in vitro (PubMed : 10024882, PubMed : 17466626, PubMed : 9771713). Required for transcription-coupled nucleotide excision repair (NER) of damaged DNA; recognizes damaged bases (PubMed : 17466626, PubMed : 23352696, PubMed : 9771713). Sequestered in chromatin on UV-damaged DNA (PubMed : 23352696). When complexed to CDK-activating kinase (CAK), involved in transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATP-dependent helicase activity of XPD/ERCC2 is required for DNA opening. Involved in DNA lesion verification (PubMed : 31253769). In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. XPD/ERCC2 acts by forming a bridge between CAK and the core-TFIIH complex. The structure of the TFIIH transcription complex differs from the NER-TFIIH complex; large movements by XPD/ERCC2 and XPB/ERCC3 are stabilized by XPA which allow this subunit to contact ssDNA (PubMed : 31253769, PubMed : 33902107). Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers.
See full target information ERCC2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

EMBO molecular medicine 15:e17836 PubMed37766669

2023

Propionate reinforces epithelial identity and reduces aggressiveness of lung carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Vignesh Ramesh,Paradesi Naidu Gollavilli,Luisa Pinna,Mohammad Aarif Siddiqui,Adriana Martinez Turtos,Francesca Napoli,Yasmin Antonelli,Aldo Leal-Egaña,Jesper Foged Havelund,Simon Toftholm Jakobsen,Elisa Le Boiteux,Marco Volante,Nils Joakim Faergeman,Ole N Jensen,Rasmus Siersbaek,Kumar Somyajit,Paolo Ceppi

Oncology letters 20:212 PubMed32963618

2020

ERCC overexpression associated with a poor response of cT4b colorectal cancer with FOLFOX-based neoadjuvant concurrent chemoradiation.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Yii Huang,Hsin-Hua Lee,Ching-Wen Huang,Chun-Ming Huang,Cheng-Jen Ma,Tzu-Chieh Yin,Hsiang-Lin Tsai,Chee-Yin Chai,Yi-Ting Chen,Jaw-Yuan Wang
View all publications
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Product promise

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