Rabbit Recombinant Monoclonal XPD antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (AB238930), expandable thumbnail](https://content.abcam.com/products/images/xpd-antibody-epr9675-bsa-and-azide-free-ab238930--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131934.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (AB238930)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (AB238930), expandable thumbnail](https://content.abcam.com/products/images/xpd-antibody-epr9675-bsa-and-azide-free-ab238930--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115656.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (AB238930)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (AB238930), expandable thumbnail](https://content.abcam.com/products/images/xpd-antibody-epr9675-bsa-and-azide-free-ab238930--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122034.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (AB238930)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (AB238930), expandable thumbnail](https://content.abcam.com/products/images/xpd-antibody-epr9675-bsa-and-azide-free-ab238930--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132713.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (AB238930)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (AB238930), expandable thumbnail](https://content.abcam.com/products/images/xpd-antibody-epr9675-bsa-and-azide-free-ab238930--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131684.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (AB238930)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
2 products for Alternative Version
Target data
Function
ATP-dependent 5'-3' DNA helicase (PubMed:31253769, PubMed:8413672, PubMed:9771713). Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, not absolutely essential for minimal transcription in vitro (PubMed:10024882, PubMed:17466626, PubMed:9771713). Required for transcription-coupled nucleotide excision repair (NER) of damaged DNA; recognizes damaged bases (PubMed:17466626, PubMed:23352696, PubMed:9771713). Sequestered in chromatin on UV-damaged DNA (PubMed:23352696). When complexed to CDK-activating kinase (CAK), involved in transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATP-dependent helicase activity of XPD/ERCC2 is required for DNA opening. Involved in DNA lesion verification (PubMed:31253769). In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. XPD/ERCC2 acts by forming a bridge between CAK and the core-TFIIH complex. The structure of the TFIIH transcription complex differs from the NER-TFIIH complex; large movements by XPD/ERCC2 and XPB/ERCC3 are stabilized by XPA which allow this subunit to contact ssDNA (PubMed:31253769, PubMed:33902107). Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers.
Alternative names
XPD, XPDC, ERCC2, General transcription and DNA repair factor IIH helicase subunit XPD, TFIIH subunit XPD, Basic transcription factor 2 80 kDa subunit, CXPD, DNA 5'-3' helicase XPD, DNA excision repair protein ERCC-2, DNA repair protein complementing XP-D cells, TFIIH basal transcription factor complex 80 kDa subunit, Xeroderma pigmentosum group D-complementing protein, BTF2 p80, TFIIH 80 kDa subunit, TFIIH p80
Recommended products
Rabbit Recombinant Monoclonal XPD antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9675
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab238930 is the carrier-free version of Anti-XPD antibody [EPR9675] ab167418.
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
XPD also known as ERCC2 is an essential helicase enzyme belonging to the family of ATP-dependent DNA helicases. It consists of approximately 761 amino acids and has a mass of around 87 kDa. XPD functions mechanically by unwinding double-stranded DNA which is important for various DNA repair processes. This protein is expressed ubiquitously in human tissues highlighting its critical role in maintaining genomic integrity.
- Biological function summary
XPD catalyzes the unwinding of DNA within the transcription factor IIH (TFIIH) complex. This complex facilitates DNA repair and transcription initiation. XPD participates in the nucleotide excision repair (NER) pathway where it contributes to repairing damaged DNA by excising damaged nucleotides. Its role in transcription-coupled repair and general transcription initiation underlines its importance in cellular homeostasis.
- Pathways
DNA repair and general transcription processes are significantly affected by XPD. It plays an integral role in nucleotide excision repair (NER) and transcription. Within these pathways XPD works closely with other proteins such as XPA which assists in damage verification and incision near DNA lesions. XPD's unwinding function is essential for the correct orientation of DNA during transcription and repair events ensuring accurate gene expression and stability.
- Associated diseases and disorders
XPD mutations link to xeroderma pigmentosum (XP) and trichothiodystrophy (TTD). These conditions often result in increased sensitivity to ultraviolet (UV) radiation and various developmental abnormalities. XPD mutations impair the repair of UV-induced DNA damage causing symptoms in both XP and TTD. XPD also connects with ERCC1 a protein important for repair synthesis and DNA incision highlighting its broad impact on human health when its function is compromised.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930), expandable thumbnail](https://content.abcam.com/products/images/xpd-antibody-epr9675-bsa-and-azide-free-ab238930--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131934.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930)
Anti-XPD antibody [EPR9675] ab167418 showing +ve staining in Human lung adenocarcinoma.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-XPD antibody [EPR9675] ab167418).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930), expandable thumbnail](https://content.abcam.com/products/images/xpd-antibody-epr9675-bsa-and-azide-free-ab238930--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115656.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930)
Anti-XPD antibody [EPR9675] ab167418 showing +ve staining in Human endometrial carcinoma.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-XPD antibody [EPR9675] ab167418).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930), expandable thumbnail](https://content.abcam.com/products/images/xpd-antibody-epr9675-bsa-and-azide-free-ab238930--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122034.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930)
Anti-XPD antibody [EPR9675] ab167418 showing +ve staining in Human cervical carcinoma.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-XPD antibody [EPR9675] ab167418).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930), expandable thumbnail](https://content.abcam.com/products/images/xpd-antibody-epr9675-bsa-and-azide-free-ab238930--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132713.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930)
Anti-XPD antibody [EPR9675] ab167418 showing +ve staining in Human normal brain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-XPD antibody [EPR9675] ab167418).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930), expandable thumbnail](https://content.abcam.com/products/images/xpd-antibody-epr9675-bsa-and-azide-free-ab238930--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131684.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling XPD with Anti-XPD antibody [EPR9675] ab167418 at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-XPD antibody [EPR9675] ab167418).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930), expandable thumbnail](https://content.abcam.com/products/images/xpd-antibody-epr9675-bsa-and-azide-free-ab238930--immunocytochemistry-immunofluorescence-img57427.jpg "Immunocytochemistry/ Immunofluorescence - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930)")
Immunocytochemistry/ Immunofluorescence - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930)
Immunofluorescent analysis of HeLa cells labeling XPD with Anti-XPD antibody [EPR9675] ab167418 at 1/250 dilution. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-XPD antibody [EPR9675] ab167418).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930), expandable thumbnail](https://content.abcam.com/products/images/xpd-antibody-epr9675-bsa-and-azide-free-ab238930--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img121527.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930)
Anti-XPD antibody [EPR9675] ab167418 showing +ve staining in Human ovarian carcinoma.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-XPD antibody [EPR9675] ab167418).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930), expandable thumbnail](https://content.abcam.com/products/images/xpd-antibody-epr9675-bsa-and-azide-free-ab238930--immunocytochemistry-immunofluorescence-img57427.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930), expandable thumbnail](https://content.abcam.com/products/images/xpd-antibody-epr9675-bsa-and-azide-free-ab238930--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img121527.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (ab238930)")
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