Anti-XPF antibody

• WB

Supplier Data

Western blot - Anti-XPF antibody (AB227712)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-XPF antibody (ab227712) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Predicted band size: 104 kDa

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• WB

Lab

Western blot - Anti-XPF antibody (AB227712)

False colour image of Western blot : Anti-XPF antibody staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab227712 was shown to bind specifically to XPF. A band was observed at 110 kDa in wild-type HeLa cell lysates with no signal observed at this size in ercc4 knockout cell line ab280068 (knockout cell lysate ab280127). To generate this image, wild-type and ercc4 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-XPF antibody (ab227712) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ERCC4 knockout HeLa cell lysate (<a href='/en-us/products/cell-lysates/human-ercc4-knockout-hela-cell-lysate-ab280127'>ab280127</a>)

Lane 2:

Western blot - Human ERCC4 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ercc4-knockout-hela-cell-line-ab280068'>ab280068</a>)

Lane 2:

ercc4 knockout HeLa cell lysate at 20 µg

Lane 3:

HEK-293 cell lysate at 20 µg

Lane 4:

Caco-2 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 110 kDa

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