Rabbit Polyclonal XPF antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ERCC4.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ERCC4. The exact immunogen used to generate this antibody is proprietary information. Database link Q92889
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Cow | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Catalytic component of a structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair, and which is essential for nucleotide excision repair (NER) and interstrand cross-link (ICL) repair.
Alternative names
ERCC11, XPF, ERCC4, DNA repair endonuclease XPF, DNA excision repair protein ERCC-4, DNA repair protein complementing XP-F cells, Xeroderma pigmentosum group F-complementing protein
Recommended products
Rabbit Polyclonal XPF antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ERCC4.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ERCC4. The exact immunogen used to generate this antibody is proprietary information. Database link Q92889
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
XPF also known as ERCC4 is a protein that plays a mechanical role in DNA repair by cleaving DNA at junctions between single-stranded and double-stranded DNA. It exhibits a molecular mass of approximately 103 kDa. This protein is expressed in various tissues with high levels found in the liver and kidney. XPF functions as a DNA endonuclease significantly influencing genomic stability through its activity in DNA repair processes.
- Biological function summary
The XPF protein collaborates with ERCC1 to form a heterodimeric complex important for nucleotide excision repair (NER). This complex is essential in recognizing and repairing bulky DNA adducts therefore maintaining DNA integrity. The complex performs specific incisions near DNA damage sites removing lesions that frequently occur due to environmental factors like UV radiation and chemical pollutants.
- Pathways
XPF functions within the NER and interstrand crosslink repair pathways. In these pathways the XPF-ERCC1 complex coordinates with other proteins like XPA and RPA to accurately excise damaged DNA segments and facilitate repair synthesis. These interactions are important for restoring normal DNA configuration and function after damage thereby preventing mutations and genomic instability.
- Associated diseases and disorders
Mutations in XPF are linked to xeroderma pigmentosum group F (XP-F) and the Cockayne syndrome. XP-F is a disorder characterized by extreme sensitivity to sunlight and an increased risk of skin cancer due to impaired DNA repair capability. In Cockayne syndrome patients experience growth defects and neurological degeneration. Both of these disorders involve dysfunctional DNA repair mechanisms where proteins like ERCC1 and others in the repair pathways fail to adequately compensate for the defective XPF function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-XPF antibody (ab227712)
5% SDS-PAGE gel.
All lanes: Western blot - Anti-XPF antibody (ab227712) at 1/1000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg
Lane 2: A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg
Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg
Predicted band size: 104 kDa
Western blot - Anti-XPF antibody (ab227712)
False colour image of Western blot: Anti-XPF antibody staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab227712 was shown to bind specifically to XPF. A band was observed at 110 kDa in wild-type HeLa cell lysates with no signal observed at this size in ercc4 knockout cell line Human ERCC4 knockout HeLa cell line ab280068 (knockout cell lysate Human ERCC4 knockout HeLa cell lysate ab280127). To generate this image, wild-type and ercc4 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-XPF antibody (ab227712) at 1/500 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: Western blot - Human ERCC4 knockout HeLa cell lysate (Human ERCC4 knockout HeLa cell lysate ab280127)
Lane 2: Western blot - Human ERCC4 knockout HeLa cell line (Human ERCC4 knockout HeLa cell line ab280068)
Lane 2: ercc4 knockout HeLa cell lysate at 20 µg
Lane 3: HEK-293 cell lysate at 20 µg
Lane 4: Caco-2 cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 110 kDa
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