Anti-XPF antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPF antibody (AB76948)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling XPF with ab76948 at 1/200 (1µg/ml). Detection : DAB.

• IP

Unknown

Immunoprecipitation - Anti-XPF antibody (AB76948)

ab76948 at 1µg/ml staining XPF in HeLa whole cell lysate immunoprecipitated using ab76948 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

All lanes:

Immunoprecipitation - Anti-XPF antibody (ab76948)

Predicted band size: 104 kDa

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• WB

Lab

Western blot - Anti-XPF antibody (AB76948)

False colour image of Western blot : Anti-XPF antibody staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab76948 was shown to bind specifically to XPF. A band was observed at 105 kDa in wild-type HeLa cell lysates with no signal observed at this size in ercc4 knockout cell line ab280068 (knockout cell lysate ab280127). To generate this image, wild-type and ercc4 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-XPF antibody (ab76948) at 1/2000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ercc4 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ERCC4 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ercc4-knockout-hela-cell-line-ab280068'>ab280068</a>)

Lane 3:

HEK-293 cell lysate at 20 µg

Lane 4:

Caco-2 cell lysate at 20 µg

Predicted band size: 104 kDa

Observed band size: 105 kDa

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• WB

Unknown

Western blot - Anti-XPF antibody (AB76948)

All lanes:

Western blot - Anti-XPF antibody (ab76948) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 104 kDa

Observed band size: 117 kDa

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