Rabbit Polyclonal XPF antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human ERCC4.
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- Science advances 7:2021Exploiting the acquired vulnerability of cisplatin-resistant tumors with a hypoxia-amplifying DNA repair-inhibiting (HYDRI) nanomedicine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Chen,Xue Wang,Yuan Yuan,Haoting Chen,Lingpu Zhang,Haihua Xiao,Jingqi Chen,Yongxiang Zhao,Jin Chang,Weisheng Guo,Xing-Jie LiangPubMed 33771859
- Cell reports 32:1081662020BRD4 Prevents R-Loop Formation and Transcription-Replication Conflicts by Ensuring Efficient Transcription Elongation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDrake S Edwards,Rohin Maganti,Jarred P Tanksley,Jie Luo,James J H Park,Elena Balkanska-Sinclair,Jinjie Ling,Scott R FloydPubMed 32966794
- Toxicology letters 333:269-2782020Differential response of human T-lymphocytes to arsenic and uranium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErica J Dashner-Titus,Jodi R Schilz,Karen A Simmons,Tammi R Duncan,Sandra C Alvarez,Laurie G HudsonPubMed 32866568
- Cell reports 28:3167-3181.e62019Dual Processing of R-Loops and Topoisomerase I Induces Transcription-Dependent DNA Double-Strand Breaks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAgnese Cristini,Giulia Ricci,Sébastien Britton,Simona Salimbeni,Shar-Yin Naomi Huang,Jessica Marinello,Patrick Calsou,Yves Pommier,Gilles Favre,Giovanni Capranico,Natalia Gromak,Olivier SordetPubMed 31533039
- Oncology letters 18:145-1522019MicroRNA-200c reverses drug resistance of human gastric cancer cells by targeting regulation of the NER-ERCC3/4 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Li et. al.PubMed 31289483
- BMC medical genetics 19:72018Fanconi anemia with sun-sensitivity caused by a Xeroderma pigmentosum-associated missense mutation in XPF.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIsabell Popp et. al.PubMed 29325523
- PloS one 7:e515632012Reduced proficiency in homologous recombination underlies the high sensitivity of embryonal carcinoma testicular germ cell tumors to Cisplatin and poly (adp-ribose) polymerase inhibition.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ERCC4. The exact immunogen used to generate this antibody is proprietary information. Database link Q92889
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Expected |
| Chimpanzee | Predicted | Predicted | Predicted | Predicted |
| Gorilla | Predicted | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla | Dilution info - | Notes - |
Associated Products
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Target data
Function
Catalytic component of a structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair, and which is essential for nucleotide excision repair (NER) and interstrand cross-link (ICL) repair.
Alternative names
ERCC11, XPF, ERCC4, DNA repair endonuclease XPF, DNA excision repair protein ERCC-4, DNA repair protein complementing XP-F cells, Xeroderma pigmentosum group F-complementing protein
Recommended products
Rabbit Polyclonal XPF antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human ERCC4.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ERCC4. The exact immunogen used to generate this antibody is proprietary information. Database link Q92889
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab76948 was affinity purified using an epitope specific to XPF immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
XPF also known as ERCC4 is a protein that plays a mechanical role in DNA repair by cleaving DNA at junctions between single-stranded and double-stranded DNA. It exhibits a molecular mass of approximately 103 kDa. This protein is expressed in various tissues with high levels found in the liver and kidney. XPF functions as a DNA endonuclease significantly influencing genomic stability through its activity in DNA repair processes.
- Biological function summary
The XPF protein collaborates with ERCC1 to form a heterodimeric complex important for nucleotide excision repair (NER). This complex is essential in recognizing and repairing bulky DNA adducts therefore maintaining DNA integrity. The complex performs specific incisions near DNA damage sites removing lesions that frequently occur due to environmental factors like UV radiation and chemical pollutants.
- Pathways
XPF functions within the NER and interstrand crosslink repair pathways. In these pathways the XPF-ERCC1 complex coordinates with other proteins like XPA and RPA to accurately excise damaged DNA segments and facilitate repair synthesis. These interactions are important for restoring normal DNA configuration and function after damage thereby preventing mutations and genomic instability.
- Associated diseases and disorders
Mutations in XPF are linked to xeroderma pigmentosum group F (XP-F) and the Cockayne syndrome. XP-F is a disorder characterized by extreme sensitivity to sunlight and an increased risk of skin cancer due to impaired DNA repair capability. In Cockayne syndrome patients experience growth defects and neurological degeneration. Both of these disorders involve dysfunctional DNA repair mechanisms where proteins like ERCC1 and others in the repair pathways fail to adequately compensate for the defective XPF function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-XPF antibody (ab76948)
False colour image of Western blot: Anti-XPF antibody staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab76948 was shown to bind specifically to XPF. A band was observed at 105 kDa in wild-type HeLa cell lysates with no signal observed at this size in ercc4 knockout cell line Human ERCC4 knockout HeLa cell line ab280068 (knockout cell lysate Human ERCC4 knockout HeLa cell lysate ab280127). To generate this image, wild-type and ercc4 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-XPF antibody (ab76948) at 1/2000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: ercc4 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human ERCC4 knockout HeLa cell line (Human ERCC4 knockout HeLa cell line ab280068)
Lane 3: HEK-293 cell lysate at 20 µg
Lane 4: Caco-2 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 104 kDa
Observed band size: 105 kDa
Western blot - Anti-XPF antibody (ab76948)
All lanes: Western blot - Anti-XPF antibody (ab76948) at 0.04 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: 293T whole cell lysate at 50 µg
Predicted band size: 104 kDa
Observed band size: 117 kDa
Immunoprecipitation - Anti-XPF antibody (ab76948)
ab76948 at 1µg/ml staining XPF in HeLa whole cell lysate immunoprecipitated using ab76948 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
All lanes: Immunoprecipitation - Anti-XPF antibody (ab76948)
Predicted band size: 104 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPF antibody (ab76948)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling XPF with ab76948 at 1/200 (1µg/ml). Detection: DAB.
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