AB46

Anti-XPG antibody [8H7]

Name: Anti-XPG antibody [8H7] (ab46) | Abcam
Brand: Abcam Limited
SKU: AB46
Price: 505 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(4 Publications)

Mouse Monoclonal XPG antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ERCC5.

View Alternative Names

ERCM2, XPG, XPGC, ERCC5, DNA excision repair protein ERCC-5, DNA repair protein complementing XP-G cells, Xeroderma pigmentosum group G-complementing protein

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8H7

Isotype

IgG2a

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human ERCC5. The exact immunogen used to generate this antibody is proprietary information.

P28715

Epitope

8H7 binds between human XPG residues Ser 947 and Ala 1165.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

Works well on crude cell extract.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

XPG also known as ERCC5 is an important player in DNA repair mechanisms specifically involved in nucleotide excision repair (NER). It is an endonuclease meaning it cuts DNA at specific sites to help remove damaged DNA. XPG is known to have a molecular weight of approximately 133 kDa. The protein is expressed in a wide array of human tissues making its function important in maintaining genomic stability across different cell types.

Biological function summary

XPG contributes to the repair of bulky DNA lesions such as those caused by UV light. This protein functions as part of the NER complex where it performs a critical incision on the DNA strand undergoing repair. In this complex XPG works alongside other proteins like XPA and RPA coordinating the removal and repair of damaged nucleotides. Its activity is essential in correcting DNA damage and maintaining cellular health protecting against mutations that might occur from environmental damage.

Pathways

XPG plays integral roles in the nucleotide excision repair pathway. This pathway is significant for correcting DNA damage induced by ultraviolet radiation and chemical agents. XPG works closely with protein ERCC1-XPF to facilitate DNA repair highlighting its collaborative function. Moreover XPG involvement extends to the base excision repair pathway further emphasizing its importance in maintaining DNA integrity.

XPG mutations are associated with disorders such as Xeroderma Pigmentosum (XP) and Cockayne Syndrome (CS). These conditions result from impaired DNA repair due to a dysfunctional NER pathway. In XP for example individuals become highly sensitive to UV exposure leading to increased cancer risk while CS leads to neurological and developmental deficits. XPG's interaction with the protein CSA becomes particularly relevant in Cockayne Syndrome indicating a vital connection in its pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Single-stranded structure-specific DNA endonuclease involved in DNA excision repair (PubMed : 32522879, PubMed : 32821917, PubMed : 7651464, PubMed : 8078765, PubMed : 8090225, PubMed : 8206890). Makes the 3'incision in DNA nucleotide excision repair (NER) (PubMed : 32522879, PubMed : 32821917, PubMed : 8078765, PubMed : 8090225). Binds and bends DNA repair bubble substrate and breaks base stacking at the single-strand/double-strand DNA junction of the DNA bubble (PubMed : 32522879). Plays a role in base excision repair (BER) by promoting the binding of DNA glycosylase NTHL1 to its substrate and increasing NTHL1 catalytic activity that removes oxidized pyrimidines from DNA (PubMed : 9927729). Involved in transcription-coupled nucleotide excision repair (TCR) which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes (PubMed : 16246722). Functions during the initial step of TCR in cooperation with ERCC6/CSB to recognized stalled RNA polymerase II (PubMed : 16246722). Also, stimulates ERCC6/CSB binding to the DNA repair bubble and ERCC6/CSB ATPase activity (PubMed : 16246722). Required for DNA replication fork maintenance and preservation of genomic stability (PubMed : 26833090, PubMed : 32522879). Involved in homologous recombination repair (HRR) induced by DNA replication stress by recruiting RAD51, BRCA2, and PALB2 to the damaged DNA site (PubMed : 26833090). In TFIIH stimulates the 5'-3' helicase activity of XPD/ERCC2 and the DNA translocase activity of XPB/ERCC3 (PubMed : 31253769). During HRR, binds to the replication fork with high specificity and stabilizes it (PubMed : 32522879). Also, acts upstream of HRR, to promote the release of BRCA1 from DNA (PubMed : 26833090).

See full target information ERCC5

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 82:1343-1358.e8 PubMed35271816

2022

Active DNA damage eviction by HLTF stimulates nucleotide excision repair.

Applications

Unspecified application

Species

Unspecified reactive species

Marvin van Toorn,Yasemin Turkyilmaz,Sueji Han,Di Zhou,Hyun-Suk Kim,Irene Salas-Armenteros,Mihyun Kim,Masaki Akita,Franziska Wienholz,Anja Raams,Eunjin Ryu,Sukhyun Kang,Arjan F Theil,Karel Bezstarosti,Maria Tresini,Giuseppina Giglia-Mari,Jeroen A Demmers,Orlando D Schärer,Jun-Hyuk Choi,Wim Vermeulen,Jurgen A Marteijn

International journal of molecular sciences 20: PubMed31731785

2019

The Effects of Meldonium on the Renal Acute Ischemia/Reperfusion Injury in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Siniša Đurašević,Maja Stojković,Ljiljana Bogdanović,Slađan Pavlović,Slavica Borković-Mitić,Ilijana Grigorov,Desanka Bogojević,Nebojša Jasnić,Tomislav Tosti,Saša Đurović,Jelena Đorđević,Zoran Todorović

Cell cycle (Georgetown, Tex.) 10:1998-2007 PubMed21558802

2011

The DNA repair endonuclease XPG interacts directly and functionally with the WRN helicase defective in Werner syndrome.

Applications

Unspecified application

Species

Human

Kelly S Trego,Sophia B Chernikova,Albert R Davalos,J Jefferson P Perry,L David Finger,Cliff Ng,Miaw-Sheue Tsai,Steven M Yannone,John A Tainer,Judith Campisi,Priscilla K Cooper

Oligonucleotides 16:94-104 PubMed16584298

2006

Involvement of ERCC1/XPF and XPG in oligodeoxynucleotide-directed gene modification.

Applications

Species

Human

Olga Igoucheva,Vitali Alexeev,Orlando Scharer,Kyonggeun Yoon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-XPG antibody [8H7]

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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Product promise