AB224815

Anti-XPG antibody

Name: Anti-XPG antibody (ab224815) | Abcam
Brand: Abcam Limited
SKU: AB224815
Price: 515 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal XPG antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ERCC5 aa 650-700.

View Alternative Names

ERCM2, XPG, XPGC, ERCC5, DNA excision repair protein ERCC-5, DNA repair protein complementing XP-G cells, Xeroderma pigmentosum group G-complementing protein

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPG antibody (AB224815)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPG antibody (AB224815)

Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for XPG using ab224815 at 1/200 dilution in immunohistochemical analysis. Detection : DAB staining.

Supplier Data

Immunoprecipitation - Anti-XPG antibody (AB224815)

XPG was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab224815 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab224815 at 1 μg/ml.

Lane 1 : ab224815 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-XPG antibody (ab224815)

Predicted band size: 133 kDa

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Supplier Data

Western blot - Anti-XPG antibody (AB224815)

All lanes:

Western blot - Anti-XPG antibody (ab224815) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg

Predicted band size: 133 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, IP

applications

Immunogen

Synthetic Peptide within Human ERCC5 aa 650-700. The exact immunogen used to generate this antibody is proprietary information.

P28715

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

XPG also known as ERCC5 is an important player in DNA repair mechanisms specifically involved in nucleotide excision repair (NER). It is an endonuclease meaning it cuts DNA at specific sites to help remove damaged DNA. XPG is known to have a molecular weight of approximately 133 kDa. The protein is expressed in a wide array of human tissues making its function important in maintaining genomic stability across different cell types.

Biological function summary

XPG contributes to the repair of bulky DNA lesions such as those caused by UV light. This protein functions as part of the NER complex where it performs a critical incision on the DNA strand undergoing repair. In this complex XPG works alongside other proteins like XPA and RPA coordinating the removal and repair of damaged nucleotides. Its activity is essential in correcting DNA damage and maintaining cellular health protecting against mutations that might occur from environmental damage.

Pathways

XPG plays integral roles in the nucleotide excision repair pathway. This pathway is significant for correcting DNA damage induced by ultraviolet radiation and chemical agents. XPG works closely with protein ERCC1-XPF to facilitate DNA repair highlighting its collaborative function. Moreover XPG involvement extends to the base excision repair pathway further emphasizing its importance in maintaining DNA integrity.

XPG mutations are associated with disorders such as Xeroderma Pigmentosum (XP) and Cockayne Syndrome (CS). These conditions result from impaired DNA repair due to a dysfunctional NER pathway. In XP for example individuals become highly sensitive to UV exposure leading to increased cancer risk while CS leads to neurological and developmental deficits. XPG's interaction with the protein CSA becomes particularly relevant in Cockayne Syndrome indicating a vital connection in its pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Single-stranded structure-specific DNA endonuclease involved in DNA excision repair (PubMed : 32522879, PubMed : 32821917, PubMed : 7651464, PubMed : 8078765, PubMed : 8090225, PubMed : 8206890). Makes the 3'incision in DNA nucleotide excision repair (NER) (PubMed : 32522879, PubMed : 32821917, PubMed : 8078765, PubMed : 8090225). Binds and bends DNA repair bubble substrate and breaks base stacking at the single-strand/double-strand DNA junction of the DNA bubble (PubMed : 32522879). Plays a role in base excision repair (BER) by promoting the binding of DNA glycosylase NTHL1 to its substrate and increasing NTHL1 catalytic activity that removes oxidized pyrimidines from DNA (PubMed : 9927729). Involved in transcription-coupled nucleotide excision repair (TCR) which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes (PubMed : 16246722). Functions during the initial step of TCR in cooperation with ERCC6/CSB to recognized stalled RNA polymerase II (PubMed : 16246722). Also, stimulates ERCC6/CSB binding to the DNA repair bubble and ERCC6/CSB ATPase activity (PubMed : 16246722). Required for DNA replication fork maintenance and preservation of genomic stability (PubMed : 26833090, PubMed : 32522879). Involved in homologous recombination repair (HRR) induced by DNA replication stress by recruiting RAD51, BRCA2, and PALB2 to the damaged DNA site (PubMed : 26833090). In TFIIH stimulates the 5'-3' helicase activity of XPD/ERCC2 and the DNA translocase activity of XPB/ERCC3 (PubMed : 31253769). During HRR, binds to the replication fork with high specificity and stabilizes it (PubMed : 32522879). Also, acts upstream of HRR, to promote the release of BRCA1 from DNA (PubMed : 26833090).

See full target information ERCC5

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106862 PubMed37275516

2023

macrophages promote hepatocellular carcinoma tumor stemness and progression through /β-catenin/ axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Chao Zhao,Chang-Zhou Chen,Yan-Zhang Tian,Huang-Qin Song,Xiao-Xiao Wang,Yan-Jun Li,Jie-Feng He,Hao-Liang Zhao

Cell reports 32:108166 PubMed32966794

2020

BRD4 Prevents R-Loop Formation and Transcription-Replication Conflicts by Ensuring Efficient Transcription Elongation.

Applications

Unspecified application

Species

Unspecified reactive species

Drake S Edwards,Rohin Maganti,Jarred P Tanksley,Jie Luo,James J H Park,Elena Balkanska-Sinclair,Jinjie Ling,Scott R Floyd

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-XPG antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPG antibody (AB224815)

Immunoprecipitation - Anti-XPG antibody (AB224815)

Western blot - Anti-XPG antibody (AB224815)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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