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AB302615

Anti-XPG antibody [EPR25351-5]

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Rabbit Recombinant Monoclonal XPG antibody. Suitable for Flow Cyt (Intra), ICC/IF, WB and reacts with Human samples.

View Alternative Names

ERCM2, XPG, XPGC, ERCC5, DNA excision repair protein ERCC-5, DNA repair protein complementing XP-G cells, Xeroderma pigmentosum group G-complementing protein

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-XPG antibody [EPR25351-5] (AB302615)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-XPG antibody [EPR25351-5] (AB302615)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling XPG with ab302615 at 1/100 (5.03 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing nuclear staining in HeLa cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

Flow Cytometry (Intracellular) - Anti-XPG antibody [EPR25351-5] (AB302615)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-XPG antibody [EPR25351-5] (AB302615)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling XPG with ab302615 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Western blot - Anti-XPG antibody [EPR25351-5] (AB302615)
  • WB

Supplier Data

Western blot - Anti-XPG antibody [EPR25351-5] (AB302615)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Samples are non-boiled as boiling may cause protein aggregates. Exposure time : Lane 1 : 103 seconds; Lane 2 : 180 seconds.

All lanes:

Western blot - Anti-XPG antibody [EPR25351-5] (ab302615) at 1/1000 dilution

Lane 1:

Human thymus tissue lysate 20 µg

Lane 2:

Human ovary cancer tissue lysate 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

Observed band size: 133 kDa

false

Western blot - Anti-XPG antibody [EPR25351-5] (AB302615)
  • WB

Supplier Data

Western blot - Anti-XPG antibody [EPR25351-5] (AB302615)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Samples are non-boiled as boiling may cause protein aggregates. Exposure time : 26 seconds

All lanes:

Western blot - Anti-XPG antibody [EPR25351-5] (ab302615) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting XPG, whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 133 kDa

false

Exposure time: 26s

Western blot - Anti-XPG antibody [EPR25351-5] (AB302615)
  • WB

Supplier Data

Western blot - Anti-XPG antibody [EPR25351-5] (AB302615)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Samples are non-boiled as boiling may cause protein aggregates. Exposure time : 180 seconds.

All lanes:

Western blot - Anti-XPG antibody [EPR25351-5] (ab302615) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 µg

Lane 2:

Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate 20 µg

Lane 3:

HaCaT (human skin keratinocyte) whole cell lysate 20 µg

Lane 4:

SK-OV-3 (human ovarian cancer epithelial cell) whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 133 kDa

false

Exposure time: 180s

  • Carrier free

    Anti-XPG antibody [EPR25351-5] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25351-5

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

XPG also known as ERCC5 is an important player in DNA repair mechanisms specifically involved in nucleotide excision repair (NER). It is an endonuclease meaning it cuts DNA at specific sites to help remove damaged DNA. XPG is known to have a molecular weight of approximately 133 kDa. The protein is expressed in a wide array of human tissues making its function important in maintaining genomic stability across different cell types.
Biological function summary

XPG contributes to the repair of bulky DNA lesions such as those caused by UV light. This protein functions as part of the NER complex where it performs a critical incision on the DNA strand undergoing repair. In this complex XPG works alongside other proteins like XPA and RPA coordinating the removal and repair of damaged nucleotides. Its activity is essential in correcting DNA damage and maintaining cellular health protecting against mutations that might occur from environmental damage.

Pathways

XPG plays integral roles in the nucleotide excision repair pathway. This pathway is significant for correcting DNA damage induced by ultraviolet radiation and chemical agents. XPG works closely with protein ERCC1-XPF to facilitate DNA repair highlighting its collaborative function. Moreover XPG involvement extends to the base excision repair pathway further emphasizing its importance in maintaining DNA integrity.

XPG mutations are associated with disorders such as Xeroderma Pigmentosum (XP) and Cockayne Syndrome (CS). These conditions result from impaired DNA repair due to a dysfunctional NER pathway. In XP for example individuals become highly sensitive to UV exposure leading to increased cancer risk while CS leads to neurological and developmental deficits. XPG's interaction with the protein CSA becomes particularly relevant in Cockayne Syndrome indicating a vital connection in its pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Single-stranded structure-specific DNA endonuclease involved in DNA excision repair (PubMed : 32522879, PubMed : 32821917, PubMed : 7651464, PubMed : 8078765, PubMed : 8090225, PubMed : 8206890). Makes the 3'incision in DNA nucleotide excision repair (NER) (PubMed : 32522879, PubMed : 32821917, PubMed : 8078765, PubMed : 8090225). Binds and bends DNA repair bubble substrate and breaks base stacking at the single-strand/double-strand DNA junction of the DNA bubble (PubMed : 32522879). Plays a role in base excision repair (BER) by promoting the binding of DNA glycosylase NTHL1 to its substrate and increasing NTHL1 catalytic activity that removes oxidized pyrimidines from DNA (PubMed : 9927729). Involved in transcription-coupled nucleotide excision repair (TCR) which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes (PubMed : 16246722). Functions during the initial step of TCR in cooperation with ERCC6/CSB to recognized stalled RNA polymerase II (PubMed : 16246722). Also, stimulates ERCC6/CSB binding to the DNA repair bubble and ERCC6/CSB ATPase activity (PubMed : 16246722). Required for DNA replication fork maintenance and preservation of genomic stability (PubMed : 26833090, PubMed : 32522879). Involved in homologous recombination repair (HRR) induced by DNA replication stress by recruiting RAD51, BRCA2, and PALB2 to the damaged DNA site (PubMed : 26833090). In TFIIH stimulates the 5'-3' helicase activity of XPD/ERCC2 and the DNA translocase activity of XPB/ERCC3 (PubMed : 31253769). During HRR, binds to the replication fork with high specificity and stabilizes it (PubMed : 32522879). Also, acts upstream of HRR, to promote the release of BRCA1 from DNA (PubMed : 26833090).
See full target information ERCC5
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