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AB224211

Anti-XPNPEP3 antibody

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(1 Publication)

Rabbit Polyclonal XPNPEP3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Xaa-Pro aminopeptidase 3 aa 50-250.

View Alternative Names

Xaa-Pro aminopeptidase 3, X-Pro aminopeptidase 3, Aminopeptidase P3, APP3, XPNPEP3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPNPEP3 antibody (AB224211)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPNPEP3 antibody (AB224211)

Paraffin embedded human stomach tissue stained for XPNPEP3 with ab224211 (1/200 dilution) in immunohistochemical analysis.

Western blot - Anti-XPNPEP3 antibody (AB224211)
  • WB

Supplier Data

Western blot - Anti-XPNPEP3 antibody (AB224211)

All lanes:

Western blot - Anti-XPNPEP3 antibody (ab224211) at 1/250 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

EFO-21 cell lysate

Lane 3:

A431 (human epidermoid carcinoma cell line) cell lysate

Lane 4:

Human liver tissue lysate

Lane 5:

Human tonsil tissue lysate

Predicted band size: 57 kDa

false

Western blot - Anti-XPNPEP3 antibody (AB224211)
  • WB

Supplier Data

Western blot - Anti-XPNPEP3 antibody (AB224211)

All lanes:

Western blot - Anti-XPNPEP3 antibody (ab224211) at 1/250 dilution

Lane 1:

NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate

Predicted band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Xaa-Pro aminopeptidase 3 aa 50-250. The exact immunogen used to generate this antibody is proprietary information.

Q9NQH7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

XPNPEP3 also known as X-prolyl aminopeptidase 3 is a mitochondrial enzyme with a mass of approximately 55 kDa. This enzyme specifically cleaves N-terminal proline from peptides playing an important role in peptide metabolism. XPNPEP3 is widely expressed with notable presence in kidney and skeletal muscle tissues. Researchers frequently study its role in cellular processes due to its involvement in the regulation of peptide-related functions.
Biological function summary

XPNPEP3 participates in maintaining cellular homeostasis by processing peptides for further cellular activities. This enzyme works as an independent entity and is not typically part of a larger protein complex. Its role in proteolysis helps mediate cellular responses to metabolic stress by breaking down specific target peptides ensuring proper peptide recycling and utilization within cells. These functions contribute to overall organismal health and metabolic efficiency.

Pathways

The enzyme XPNPEP3 takes part in the mitochondrial processing pathway which involves the degradation and modification of mitochondrial peptides. It is closely linked to the activity of other mitochondrial enzymes such as MPP (mitochondrial processing peptidase). XPNPEP3's actions fit within the broader context of the mitochondrial function impacting pathways responsible for energy production and mitochondrial homeostasis ensuring proper cellular functionality.

Studies associate XPNPEP3 with nephronophthisis-like kidney disorder and related ciliopathies. Mutations affecting XPNPEP3 expression or function can lead to improper peptide processing contributing to the pathogenesis of these conditions. The enzyme operates alongside proteins involved in renal development and function such as NEK8 highlighting its important role in maintaining healthy kidney tissue. Understanding XPNPEP3's contributions can offer insights into potential therapeutic approaches for such disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Leu-Pro-Ala (PubMed : 25609706, PubMed : 28476889). Also shows low activity towards peptides with Ala or Ser at the P1 position (PubMed : 28476889).. Isoform 1. Promotes TNFRSF1B-mediated phosphorylation of MAPK8/JNK1 and MAPK9/JNK2, suggesting a function as an adapter protein for TNFRSF1B; the effect is independent of XPNPEP3 peptidase activity. May inhibit apoptotic cell death induced via TNF-TNFRSF1B signaling.
See full target information Xaa-Pro aminopeptidase 3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 15:e70464 PubMed40985995

2025

CD103CD8 tissue-resident memory T lymphocytes of melanoma boost anti-tumour immunity and predict immunotherapy outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyi Zhang,Junquan Song,Yinlam Li,Kangjie Shen,Jiangying Xuan,Yuan Gao,Lili Lu,Zhi Pang,Lu Wang,Yang Yang,Zixu Gao,Qianrong Hu,Yu Zhu,Chenlu Wei,Shaoluan Zheng,Rongkui Luo,Yingyong Hou,Yuhong Zhou,Chuanyuan Wei,Jianying Gu
View all publications
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