Anti-XPR1 antibody [HL2631] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-XPR1 antibody [HL2631] - BSA and Azide free (AB325877)

ab325877 detects XPR1 protein at cell membrane by immunofluorescent analysis. Sample : HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green : XPR1 stained by ab325877 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

• WB

Supplier Data

Western blot - Anti-XPR1 antibody [HL2631] - BSA and Azide free (AB325877)

Boiled and unboiled HCT116 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab325877 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-XPR1 antibody [HL2631] - BSA and Azide free (ab325877) at 1/1000 dilution

Lane 1:

Boiled HCT116 whole cell extracts at 30 µg

Lane 2:

Unboiled HCT116 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-XPR1 antibody [HL2631] - BSA and Azide free (AB325877)

Boiled and unboiled mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab325877 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

All lanes:

Western blot - Anti-XPR1 antibody [HL2631] - BSA and Azide free (ab325877) at 1/1000 dilution

Lane 1:

Boiled mouse brain tissue extract at 50 µg

Lane 2:

Unboiled mouse brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-XPR1 antibody [HL2631] - BSA and Azide free (AB325877)

Boiled and unboiled mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with ab325877 diluted at 1 : 1000 and competitor's antibody diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-XPR1 antibody [HL2631] - BSA and Azide free (ab325877) at 1/1000 dilution

Lane 1:

Boiled mouse brain tissue extract at 50 µg

Lane 2:

Unboiled mouse brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-XPR1 antibody [HL2631] - BSA and Azide free (AB325877)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab325877 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-XPR1 antibody [HL2631] - BSA and Azide free (ab325877) at 1/1000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged XPR1 transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged XPR1 transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-XPR1 antibody [HL2631] - BSA and Azide free (AB325877)

Non-transfected (–) and transfected (+) unboiled HCT-116 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab325877 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-XPR1 antibody [HL2631] - BSA and Azide free (ab325877) at 1/1000 dilution

Lane 1:

Non-transfected (–) unboiled HCT-116 whole cell extract at 30 µg

Lane 2:

XPR1 shRNA transfected (+) unboiled HCT-116 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-XPR1 antibody [HL2631] - BSA and Azide free (AB325877)

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with ab325877 diluted at 1 : 1000 and competitor's antibody diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-XPR1 antibody [HL2631] - BSA and Azide free (ab325877) at 1/1000 dilution

Lane 1:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged XPR1 transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false