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AB235196

Anti-XRCC1 antibody

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(2 Publications)

Chicken Polyclonal XRCC1 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications.

View Alternative Names

DNA repair protein XRCC1, X-ray repair cross-complementing protein 1, XRCC1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody (AB235196)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody (AB235196)

100% methanol-fixed, 0.3% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling XRCC1 using ab235196 at 10 μg/ml, overnight at +4°C. Followed by a Donkey anti-Chicken IgG DyLight™ 488 conjugated preadsorbed secondary antibody at 5 μg/ml, for 1 hour at RT.

Western blot - Anti-XRCC1 antibody (AB235196)
  • WB

Supplier Data

Western blot - Anti-XRCC1 antibody (AB235196)

All lanes:

Western blot - Anti-XRCC1 antibody (ab235196) at 5 µg/mL

Lane 1:

U-2 OS whole cell lysate at 35 µg

Lane 2:

Molt4 whole cell lysate at 35 µg

Lane 3:

Human testis whole tissue lysate at 35 µg

Lane 4:

Mouse liver whole tissue lysate at 35 µg

Secondary

All lanes:

Goat anti-Chicken IgG [H&L] at 1/40000 dilution

Predicted band size: 69 kDa

false

Exposure time: 20s

Key facts

Host species

Chicken

Clonality

Polyclonal

Isotype

IgY

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

XRCC1 (X-ray repair cross-complementing protein 1) plays a role in DNA repair by acting as a scaffold protein. It weighs approximately 70 kDa and is expressed in various tissues reflecting its role in maintaining genomic stability. XRCC1 helps in the repair of single-strand breaks by coordinating the activity of several enzymes involved in the base excision repair pathway. Together with other proteins it helps ensure the integrity of DNA by fostering repair mechanisms following damage by ionizing radiation or other environmental factors.
Biological function summary

The activity of XRCC1 integrates into DNA damage response as it partners with enzymes like DNA ligase III DNA polymerase beta and poly (ADP-ribose) polymerase (PARP). This protein functions by forming complexes with these enzymes facilitating quick and accurate repair. The ability of XRCC1 to bring together repair machinery is essential for cell survival after DNA damage and plays a role in the prevention of mutagenesis and carcinogenesis as errors in repair processes could lead to genetic mutations.

Pathways

The XRCC1 protein participates in base excision repair and single-strand break repair pathways. It works closely with PARP1 and DNA ligase III to correct single-strand DNA interruptions. Through these pathways XRCC1 interacts with other repair proteins like TDP1 aiding in the resolution of complex DNA end structures. This collaboration ensures the completion of cellular repair processes maintaining genomic integrity and reducing the likelihood of accumulating harmful mutations.

Mutations in XRCC1 have links to cancer development particularly those involving increased susceptibility to tobacco-related cancers. Studies have connected XRCC1 polymorphisms to a higher risk for conditions such as breast and lung cancer. Relations between XRCC1 and disorders can also be observed with neurodegenerative conditions; for instance alterations in its function or expression patterns might amplify disease progression involving neurological decline. Moreover its interactions with proteins like PARP1 bring forth potential implications in therapeutic strategies aiming to exploit DNA repair pathways for targeted cancer treatments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes (PubMed : 11163244, PubMed : 28002403). Negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity (PubMed : 28002403, PubMed : 34102106, PubMed : 34811483). Recognizes and binds poly-ADP-ribose chains : specifically binds auto-poly-ADP-ribosylated PARP1, limiting its activity (PubMed : 14500814, PubMed : 34102106, PubMed : 34811483).
See full target information XRCC1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

General physiology and biophysics 44:303-315 PubMed40567073

2025

Protocatechuic acid alleviates chronic obstructive pulmonary disease model by reducing inflammation and oxidative stress via the AHR/CYP1A1/CYP1A2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xingyu Pan,Xiaowei Zhang,Ruizhi Li,Sheng Zhang,Chengda Zhang,Yongjia Zhu

Frontiers in cell and developmental biology 9:719192 PubMed34497808

2021

PARP1 Inhibitor Combined With Oxaliplatin Efficiently Suppresses Oxaliplatin Resistance in Gastric Cancer-Derived Organoids via Homologous Recombination and the Base Excision Repair Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Huafu Li,Chunming Wang,Linxiang Lan,Wenhui Wu,Ian Evans,E Josue Ruiz,Leping Yan,Zhijun Zhou,Joaquim M Oliveira,Rui L Reis,Zhenran Hu,Wei Chen,Axel Behrens,Yulong He,Changhua Zhang
View all publications
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