AB9147

Anti-XRCC1 antibody

Name: Anti-XRCC1 antibody (ab9147) | Abcam
Brand: Abcam Limited
SKU: AB9147
Price: 545 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(15 Publications)

Rabbit Polyclonal XRCC1 antibody. Suitable for WB and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human XRCC1 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

DNA repair protein XRCC1, X-ray repair cross-complementing protein 1, XRCC1

1 Images

Unknown

Western blot - Anti-XRCC1 antibody (AB9147)

Detection : ECL.

All lanes:

Western blot - Anti-XRCC1 antibody (ab9147) at 1 µg/mL

Lane 1:

Whole cell extract (50mcg) from mock treated HeLa cells

Lane 2:

Whole cell extract (50mcg) from siRNA treated HeLa cells

Predicted band size: 69 kDa

Observed band size: 76 kDa,90 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human XRCC1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P18887

Specificity

This antibody recognises human XRCC1 (x-ray cross complementing factor -1), a base excision repair protein.

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Reactivity data

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Product details

Recently XRCC-1 has been shown to be physically associated with other DNA repair enzymes, including PARP, DNA ligase III and DNA polymerase beta.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibodies were affinity purified using thepeptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG.

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

XRCC1 (X-ray repair cross-complementing protein 1) plays a role in DNA repair by acting as a scaffold protein. It weighs approximately 70 kDa and is expressed in various tissues reflecting its role in maintaining genomic stability. XRCC1 helps in the repair of single-strand breaks by coordinating the activity of several enzymes involved in the base excision repair pathway. Together with other proteins it helps ensure the integrity of DNA by fostering repair mechanisms following damage by ionizing radiation or other environmental factors.

Biological function summary

The activity of XRCC1 integrates into DNA damage response as it partners with enzymes like DNA ligase III DNA polymerase beta and poly (ADP-ribose) polymerase (PARP). This protein functions by forming complexes with these enzymes facilitating quick and accurate repair. The ability of XRCC1 to bring together repair machinery is essential for cell survival after DNA damage and plays a role in the prevention of mutagenesis and carcinogenesis as errors in repair processes could lead to genetic mutations.

Pathways

The XRCC1 protein participates in base excision repair and single-strand break repair pathways. It works closely with PARP1 and DNA ligase III to correct single-strand DNA interruptions. Through these pathways XRCC1 interacts with other repair proteins like TDP1 aiding in the resolution of complex DNA end structures. This collaboration ensures the completion of cellular repair processes maintaining genomic integrity and reducing the likelihood of accumulating harmful mutations.

Mutations in XRCC1 have links to cancer development particularly those involving increased susceptibility to tobacco-related cancers. Studies have connected XRCC1 polymorphisms to a higher risk for conditions such as breast and lung cancer. Relations between XRCC1 and disorders can also be observed with neurodegenerative conditions; for instance alterations in its function or expression patterns might amplify disease progression involving neurological decline. Moreover its interactions with proteins like PARP1 bring forth potential implications in therapeutic strategies aiming to exploit DNA repair pathways for targeted cancer treatments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes (PubMed : 11163244, PubMed : 28002403). Negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity (PubMed : 28002403, PubMed : 34102106, PubMed : 34811483). Recognizes and binds poly-ADP-ribose chains : specifically binds auto-poly-ADP-ribosylated PARP1, limiting its activity (PubMed : 14500814, PubMed : 34102106, PubMed : 34811483).

See full target information XRCC1

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 36:850-860 PubMed33378118

2020

The role of H3K9me2-regulated base excision repair genes in the repair of DNA damage induced by arsenic in HaCaT cells and the effects of Ginkgo biloba extract intervention.

Applications

Unspecified application

Species

Unspecified reactive species

Xuejiao Ding,Anliu Zhang,Changzhe Li,Lu Ma,Shunfang Tang,Qi Wang,Guanghong Yang,Jun Li

Cancer research 80:1914-1926 PubMed32193286

2020

Endonuclease FEN1 Coregulates ERα Activity and Provides a Novel Drug Interface in Tamoxifen-Resistant Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Koen D Flach,Manikandan Periyasamy,Ajit Jadhav,Dorjbal Dorjsuren,Joseph C Siefert,Theresa E Hickey,Mark Opdam,Hetal Patel,Sander Canisius,David M Wilson,Maria Donaldson Collier,Stefan Prekovic,Marja Nieuwland,Roelof J C Kluin,Alexey V Zakharov,Jelle Wesseling,Lodewyk F A Wessels,Sabine C Linn,Wayne D Tilley,Anton Simeonov,Simak Ali,Wilbert Zwart

World journal of gastroenterology 25:5814-5825 PubMed31636474

2019

Up-regulated Wnt1-inducible signaling pathway protein 1 correlates with poor prognosis and drug resistance by reducing DNA repair in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Hua Zhang,Yan Wang,Qian-Qian Fan,Yan-Kui Liu,Long-Hai Li,Xiao-Wei Qi,Yong Mao,Dong Hua

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 109:1541-1546 PubMed30551406

2018

Triptolide interferes with XRCC1/PARP1-mediated DNA repair and confers sensitization of triple-negative breast cancer cells to cisplatin.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwei Zhang,Caifeng Sun,Lu Zhang,Xinming Chi,Jiamei Ji,Xingjie Gao,Ying Wang,Zinan Zhao,Likun Liu,Xuejiao Cao,Yanqin Yang,Weifeng Mao

Scientific reports 6:37423 PubMed27857169

2016

Mobile phone signal exposure triggers a hormesis-like effect in Atm and Atm mouse embryonic fibroblasts.

Applications

Species

Mouse

Chuan Sun,Xiaoxia Wei,Yue Fei,Liling Su,Xinyuan Zhao,Guangdi Chen,Zhengping Xu

Proceedings of the National Academy of Sciences of 111:E1016-24 PubMed24591630

2014

Differential regulation of S-region hypermutation and class-switch recombination by noncanonical functions of uracil DNA glycosylase.

Applications

Unspecified application

Species

Unspecified reactive species

Ashraf S Yousif,Andre Stanlie,Samiran Mondal,Tasuku Honjo,Nasim A Begum

The Journal of experimental medicine 208:2209-16 PubMed21967769

2011

XRCC1 suppresses somatic hypermutation and promotes alternative nonhomologous end joining in Igh genes.

Applications

Species

Unspecified reactive species

Huseyin Saribasak,Robert W Maul,Zheng Cao,Rhonda L McClure,William Yang,Daniel R McNeill,David M Wilson,Patricia J Gearhart

Molecular and cellular biology 31:4623-32 PubMed21930793

2011

Nucleosome disruption by DNA ligase III-XRCC1 promotes efficient base excision repair.

Applications

Unspecified application

Species

Unspecified reactive species

Ian D Odell,Joy-El Barbour,Drew L Murphy,Julie A Della-Maria,Joann B Sweasy,Alan E Tomkinson,Susan S Wallace,David S Pederson

Cancer research 71:842-51 PubMed21123451

2010

BCR/ABL stimulates WRN to promote survival and genomic instability.

Applications

Species

Mouse

Artur Slupianek,Tomasz Poplawski,Stanislaw K Jozwiakowski,Kimberly Cramer,Dariusz Pytel,Ewelina Stoczynska,Michal O Nowicki,Janusz Blasiak,Tomasz Skorski

The Journal of urology 180:2706-9 PubMed18951558

2008

Septins as diagnostic markers for a subset of human asthenozoospermia.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshio Sugino,Kentaro Ichioka,Takeshi Soda,Masafumi Ihara,Makoto Kinoshita,Osamu Ogawa,Hiroyuki Nishiyama

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-XRCC1 antibody

Western blot - Anti-XRCC1 antibody (AB9147)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

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