Rabbit Recombinant Monoclonal XRCC1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 18 publications.
Images
![Western blot - Anti-XRCC1 antibody [EPR4389(2)] (AB134056), expandable thumbnail](https://content.abcam.com/products/images/xrcc1-antibody-epr43892-ab134056--western-blot-img24856.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-XRCC1 antibody [EPR4389(2)] (AB134056)")
![Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (AB134056), expandable thumbnail](https://content.abcam.com/products/images/xrcc1-antibody-epr43892-ab134056--immunocytochemistry-immunofluorescence-img101997.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (AB134056)")
![Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (AB134056), expandable thumbnail](https://content.abcam.com/products/images/xrcc1-antibody-epr43892-ab134056--immunocytochemistry-immunofluorescence-img71722.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (AB134056)")
![Western blot - Anti-XRCC1 antibody [EPR4389(2)] (AB134056), expandable thumbnail](https://content.abcam.com/products/images/xrcc1-antibody-epr43892-ab134056--western-blot-img106084.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-XRCC1 antibody [EPR4389(2)] (AB134056)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC1 antibody [EPR4389(2)] (AB134056), expandable thumbnail](https://content.abcam.com/products/images/xrcc1-antibody-epr43892-ab134056--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81406.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC1 antibody [EPR4389(2)] (AB134056)")
Publications
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- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e22068962023circCIMT Silencing Promotes Cadmium-Induced Malignant Transformation of Lung Epithelial Cells Through the DNA Base Excision Repair Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeizhen Li,Wei Chen,Jinjin Cui,Qiuyi Lin,Yufei Liu,Huixian Zeng,Qiuhan Hua,Yihui Ling,Xiaodi Qin,Yindai Zhang,Xueqi Li,Tianshu Lin,Lihua Huang,Yiguo JiangPubMed 36814305
- Cancer discovery 12:2586-26052022Proteogenomic Markers of Chemotherapy Resistance and Response in Triple-Negative Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeenakshi Anurag,Eric J Jaehnig,Karsten Krug,Jonathan T Lei,Erik J Bergstrom,Beom-Jun Kim,Tanmayi D Vashist,Anh Minh Tran Huynh,Yongchao Dou,Xuxu Gou,Chen Huang,Zhiao Shi,Bo Wen,Viktoriya Korchina,Richard A Gibbs,Donna M Muzny,Harshavardhan Doddapaneni,Lacey E Dobrolecki,Henry Rodriguez,Ana I Robles,Tara Hiltke,Michael T Lewis,Julie R Nangia,Maryam Nemati Shafaee,Shunqiang Li,Ian S Hagemann,Jeremy Hoog,Bora Lim,C Kent Osborne,D R Mani,Michael A Gillette,Bing Zhang,Gloria V Echeverria,George Miles,Mothaffar F Rimawi,Steven A Carr,Foluso O Ademuyiwa,Shankha Satpathy,Matthew J EllisPubMed 36001024
- BioMed research international 2022:85685282022ZSTK474 Sensitizes Glioblastoma to Temozolomide by Blocking Homologous Recombination Repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenhui Jiao,Shan Zhu,Jingrong Shao,Xiaoliang Zhang,Yanglu Xu,Yixuan Zhang,Ran Wang,Yuxu Zhong,Dexin KongPubMed 35872860
- eLife 11:2022The out-of-field dose in radiation therapy induces delayed tumorigenesis by senescence evasion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErwan Goy,Maxime Tomezak,Caterina Facchin,Nathalie Martin,Emmanuel Bouchaert,Jerome Benoit,Clementine de Schutter,Joe Nassour,Laure Saas,Claire Drullion,Priscille M Brodin,Alexandre Vandeputte,Olivier Molendi-Coste,Laurent Pineau,Gautier Goormachtigh,Olivier Pluquet,Albin Pourtier,Fabrizio Cleri,Eric Lartigau,Nicolas Penel,Corinne AbbadiePubMed 35302491
- Cancers 14:2022Repair-Assisted Damage Detection Reveals Biological Disparities in Prostate Cancer between African Americans and European Americans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKimiko L Krieger,Jie H Gohlke,Kevin J Lee,Danthasinghe Waduge Badrajee Piyarathna,Patricia D Castro,Jeffrey A Jones,Michael M Ittmann,Natalie R Gassman,Arun SreekumarPubMed 35205762
- Cell death & disease 12:11382021FDI-6 inhibits the expression and function of FOXM1 to sensitize BRCA-proficient triple-negative breast cancer cells to Olaparib by regulating cell cycle progression and DNA damage repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShu-Ping Wang,Shi-Qi Wu,Shi-Hui Huang,Yi-Xuan Tang,Liu-Qiong Meng,Feng Liu,Qi-Hua Zhu,Yun-Gen XuPubMed 34880209
- Molecular cell 81:3128-3144.e72021Replication gaps are a key determinant of PARP inhibitor synthetic lethality with BRCA deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKe Cong,Min Peng,Arne Nedergaard Kousholt,Wei Ting C Lee,Silviana Lee,Sumeet Nayak,John Krais,Pamela S VanderVere-Carozza,Katherine S Pawelczak,Jennifer Calvo,Nicholas J Panzarino,John J Turchi,Neil Johnson,Jos Jonkers,Eli Rothenberg,Sharon B CantorPubMed 34216544
- Scientific reports 11:34902021Small molecule inhibitor of OGG1 blocks oxidative DNA damage repair at telomeres and potentiates methotrexate anticancer effects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuan Miguel Baquero,Carlos Benítez-Buelga,Varshni Rajagopal,Zhao Zhenjun,Raúl Torres-Ruiz,Sarah Müller,Bishoy M F Hanna,Olga Loseva,Olov Wallner,Maurice Michel,Sandra Rodríguez-Perales,Helge Gad,Torkild Visnes,Thomas Helleday,Javier Benítez,Ana OsorioPubMed 33568707
- Cancer gene therapy 29:101-1212021Circular RNA FLNA acts as a sponge of miR-486-3p in promoting lung cancer progression via regulating XRCC1 and CYP1A1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiongwei Pan,Gang Huang,Zhangyong Yin,Xiaoping Cai,Enhui Gong,Yuling Li,Cunlai Xu,Zaiting Ye,Zhuo Cao,Wei ChengPubMed 33500536
- The Journal of neuroscience : the official journal 40:9012-90272020Suppression of DNA Double-Strand Break Formation by DNA Polymerase β in Active DNA Demethylation Is Required for Development of Hippocampal Pyramidal Neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkiko Uyeda et. al.PubMed 33087478
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Tested |
| Mouse | Expected | Not recommended | Not recommended | Tested | Tested |
| Rat | Expected | Not recommended | Not recommended | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 - 1/500 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/250 | Notes - |
Species Rat | Dilution info 1/100 - 1/250 | Notes - |
Species Human | Dilution info 1/100 - 1/250 | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes (PubMed:11163244, PubMed:28002403). Negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity (PubMed:28002403, PubMed:34102106, PubMed:34811483). Recognizes and binds poly-ADP-ribose chains: specifically binds auto-poly-ADP-ribosylated PARP1, limiting its activity (PubMed:14500814, PubMed:34102106, PubMed:34811483).
Alternative names
DNA repair protein XRCC1, X-ray repair cross-complementing protein 1, XRCC1
Recommended products
Rabbit Recombinant Monoclonal XRCC1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 18 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR4389(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
XRCC1 (X-ray repair cross-complementing protein 1) plays a role in DNA repair by acting as a scaffold protein. It weighs approximately 70 kDa and is expressed in various tissues reflecting its role in maintaining genomic stability. XRCC1 helps in the repair of single-strand breaks by coordinating the activity of several enzymes involved in the base excision repair pathway. Together with other proteins it helps ensure the integrity of DNA by fostering repair mechanisms following damage by ionizing radiation or other environmental factors.
- Biological function summary
The activity of XRCC1 integrates into DNA damage response as it partners with enzymes like DNA ligase III DNA polymerase beta and poly (ADP-ribose) polymerase (PARP). This protein functions by forming complexes with these enzymes facilitating quick and accurate repair. The ability of XRCC1 to bring together repair machinery is essential for cell survival after DNA damage and plays a role in the prevention of mutagenesis and carcinogenesis as errors in repair processes could lead to genetic mutations.
- Pathways
The XRCC1 protein participates in base excision repair and single-strand break repair pathways. It works closely with PARP1 and DNA ligase III to correct single-strand DNA interruptions. Through these pathways XRCC1 interacts with other repair proteins like TDP1 aiding in the resolution of complex DNA end structures. This collaboration ensures the completion of cellular repair processes maintaining genomic integrity and reducing the likelihood of accumulating harmful mutations.
- Associated diseases and disorders
Mutations in XRCC1 have links to cancer development particularly those involving increased susceptibility to tobacco-related cancers. Studies have connected XRCC1 polymorphisms to a higher risk for conditions such as breast and lung cancer. Relations between XRCC1 and disorders can also be observed with neurodegenerative conditions; for instance alterations in its function or expression patterns might amplify disease progression involving neurological decline. Moreover its interactions with proteins like PARP1 bring forth potential implications in therapeutic strategies aiming to exploit DNA repair pathways for targeted cancer treatments.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-XRCC1 antibody [EPR4389(2)] (ab134056), expandable thumbnail](https://content.abcam.com/products/images/xrcc1-antibody-epr43892-ab134056--western-blot-img24856.jpg "Western blot - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)")
Western blot - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)
Blocking/Diluting buffer: 5% NFDM /TBST
Exposure time:
Lanes 1-2: 3 minutes
Lanes 3-4: 20 secondsAll lanes: Western blot - Anti-XRCC1 antibody [EPR4389(2)] (ab134056) at 1/2000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse kidney lysate at 20 µg
Lane 3: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg
Lane 4: NIH/3T3(Mouse embryonic fibroblast) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated(Pierce) at 1/1000 dilution
Predicted band size: 69 kDa
Observed band size: 85 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056), expandable thumbnail](https://content.abcam.com/products/images/xrcc1-antibody-epr43892-ab134056--immunocytochemistry-immunofluorescence-img101997.jpg "Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)")
Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)
Immunocytochemistry/Immunofluorescence analysis of PC-12(Rat adrenal gland pheochromocytoma) labelling with ab134056 at a dilution of 1/50 dilution (4.66 μg/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (1/1000 dilution (2 μg/mL)) was used as the secondary antibody. The cells were co-stained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Nuclei counterstained with DAPI (blue). Control: PBS instead of the primary antibody.
![Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056), expandable thumbnail](https://content.abcam.com/products/images/xrcc1-antibody-epr43892-ab134056--immunocytochemistry-immunofluorescence-img71722.jpg "Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)")
Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)
Immunocytochemistry/Immunofluorescence analysis of NIH/3T3(Mouse embryonic fibroblast) labelling with ab134056 at a dilution of 1/50 (4.66 μg/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution (2 μg/mL)) was used as the secondary antibody. The cells were co-stained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Nuclei counterstained with DAPI (blue). Control: PBS instead of the primary antibody.
![Western blot - Anti-XRCC1 antibody [EPR4389(2)] (ab134056), expandable thumbnail](https://content.abcam.com/products/images/xrcc1-antibody-epr43892-ab134056--western-blot-img106084.jpg "Western blot - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)")
Western blot - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)
All lanes: Western blot - Anti-XRCC1 antibody [EPR4389(2)] (ab134056) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: A375 cell lysate at 10 µg
Lane 3: Saos-2 cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 69 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC1 antibody [EPR4389(2)] (ab134056), expandable thumbnail](https://content.abcam.com/products/images/xrcc1-antibody-epr43892-ab134056--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81406.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling XRCC1 with ab134056 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056), expandable thumbnail](https://content.abcam.com/products/images/xrcc1-antibody-epr43892-ab134056--immunocytochemistry-immunofluorescence-img86121.jpg "Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)")
This image is courtesy of a customer review submitted by Kirk McManusImmunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)
ab134056 staining XRCC1 in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.
![Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056), expandable thumbnail](https://content.abcam.com/products/images/xrcc1-antibody-epr43892-ab134056--immunocytochemistry-immunofluorescence-img89814.jpg "Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)")
Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)
Immunofluorescent analysis of HeLa cells labelling XRCC1 with ab134056 at 1/100 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056), expandable thumbnail](https://content.abcam.com/products/images/xrcc1-antibody-epr43892-ab134056--immunocytochemistry-immunofluorescence-img86121.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)")
![Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056), expandable thumbnail](https://content.abcam.com/products/images/xrcc1-antibody-epr43892-ab134056--immunocytochemistry-immunofluorescence-img89814.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)")
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