Anti-XRCC1 antibody [EPR4389(2)]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (AB134056)

Immunofluorescent analysis of HeLa cells labelling XRCC1 with ab134056 at 1/100 dilution.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (AB134056)

ab134056 staining XRCC1 in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.

This image is courtesy of an Abreview submitted by Kirk McManus

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (AB134056)

Immunocytochemistry/Immunofluorescence analysis of PC-12(Rat adrenal gland pheochromocytoma) labelling with ab134056 at a dilution of 1/50 dilution (4.66 μg/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat anti rabbit IgG (Alexa Fluor^® 488 ab150077) (1/1000 dilution (2 μg/mL)) was used as the secondary antibody. The cells were co-stained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Nuclei counterstained with DAPI (blue). Control : PBS instead of the primary antibody.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (AB134056)

Immunocytochemistry/Immunofluorescence analysis of NIH/3T3(Mouse embryonic fibroblast) labelling with ab134056 at a dilution of 1/50 (4.66 μg/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat anti rabbit IgG (Alexa Fluor^® 488 ab150077) at 1/1000 dilution (2 μg/mL)) was used as the secondary antibody. The cells were co-stained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Nuclei counterstained with DAPI (blue). Control : PBS instead of the primary antibody.

• WB

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Western blot - Anti-XRCC1 antibody [EPR4389(2)] (AB134056)

All lanes:

Western blot - Anti-XRCC1 antibody [EPR4389(2)] (ab134056) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

A375 cell lysate at 10 µg

Lane 3:

Saos-2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 69 kDa

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• WB

Lab

Western blot - Anti-XRCC1 antibody [EPR4389(2)] (AB134056)

Blocking/Diluting buffer : 5% NFDM /TBST

Exposure time :
Lanes 1-2 : 3 minutes
Lanes 3-4 : 20 seconds

All lanes:

Western blot - Anti-XRCC1 antibody [EPR4389(2)] (ab134056) at 1/2000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse kidney lysate at 20 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 4:

NIH/3T3(Mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated(Pierce) at 1/1000 dilution

Predicted band size: 69 kDa

Observed band size: 85 kDa

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