Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (AB226150)

This data was developed using ab134056, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labelling XRCC1 with ab134056 at 1/100 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (AB226150)

This data was developed using ab134056, the same antibody clone in a different buffer formulation.

Immunocytochemistry/Immunofluorescence analysis of PC-12(Rat adrenal gland pheochromocytoma) labelling with ab134056 at a dilution of 1/50 dilution (4.66 μg/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) (1/1000 dilution (2 μg/mL)) was used as the secondary antibody. The cells were co-stained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Nuclei counterstained with DAPI (blue). Control : PBS instead of the primary antibody.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (AB226150)

This data was developed using ab134056, the same antibody clone in a different buffer formulation.

Immunocytochemistry/Immunofluorescence analysis of NIH/3T3(Mouse embryonic fibroblast) labelling with ab134056 at a dilution of 1/50 (4.66 μg/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/1000 dilution (2 μg/mL)) was used as the secondary antibody. The cells were co-stained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Nuclei counterstained with DAPI (blue). Control : PBS instead of the primary antibody.

• WB

Unknown

Western blot - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (AB226150)

This data was developed using ab134056, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-XRCC1 antibody [EPR4389(2)] (<a href='/en-us/products/primary-antibodies/xrcc1-antibody-epr43892-ab134056'>ab134056</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

A375 cell lysate at 10 µg

Lane 3:

Saos-2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 69 kDa

false

• WB

Lab

Western blot - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (AB226150)

This data was developed using ab134056, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time :

Lanes 1-2 : 3 minutes.

Lanes 3-4 : 20 seconds.

All lanes:

Western blot - Anti-XRCC1 antibody [EPR4389(2)] (<a href='/en-us/products/primary-antibodies/xrcc1-antibody-epr43892-ab134056'>ab134056</a>) at 1/2000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse kidney lysate at 20 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 4:

NIH/3T3(Mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated(Pierce) at 1/1000 dilution

Predicted band size: 69 kDa

Observed band size: 85 kDa

false