AB180752

Anti-XRCC2 antibody

Name: Anti-XRCC2 antibody (ab180752) | Abcam
Brand: Abcam Limited
SKU: AB180752
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal XRCC2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human XRCC2.

View Alternative Names

DNA repair protein XRCC2, X-ray repair cross-complementing protein 2, XRCC2

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-XRCC2 antibody (AB180752)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-XRCC2 antibody (AB180752)

Immunocytochemistry/Immunofluorescence analysis of MCF7 cells using ab180752. Blue DAPI for nuclear staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC2 antibody (AB180752)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC2 antibody (AB180752)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue labelling XRCC2 with ab180752 at a dilution of 1/50.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-XRCC2 antibody (AB180752)

Immunocytochemistry/Immunofluorescence analysis of A549 cells labeling XRCC2 with ab180752 (shown in green) at a dilution of 1/50. Nuclear DNA was labelled with DAPI (shown in blue).

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-XRCC2 antibody (AB180752)

Immunocytochemistry/Immunofluorescence analysis of A549 cells labeling XRCC2 with ab180752 (shown in green) at a dilution of 1/50.

Supplier Data

Western blot - Anti-XRCC2 antibody (AB180752)

All lanes:

Western blot - Anti-XRCC2 antibody (ab180752) at 1/500 dilution

Lane 1:

Extracts of HeLa cell line

Lane 2:

Extracts of SH-SY5Y cell line

Lane 3:

Extracts of SW480 cell line

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human XRCC2.

O43543

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

XRCC2 also known as X-ray repair cross-complementing protein 2 is a protein that plays a role in DNA repair mechanisms. It has a molecular mass of approximately 33 kDa. XRCC2 is expressed in various tissues with noticeable abundance in proliferative tissues due to their high demand for DNA repair processes. Mechanistically XRCC2 functions as part of the Rad51 paralog group facilitating homologous recombination repair of DNA double-strand breaks. Its involvement is quintessential for maintaining genomic stability.

Biological function summary

XRCC2 contributes to cellular maintenance by repairing DNA double-strand breaks through the homologous recombination pathway. It participates in the Rad51 paralog complex alongside other paralogs like Rad51B Rad51C Rad51D and XRCC3. This complex ensures precise repair and re-ligation of damaged DNA by assisting in strand exchange and stabilization. Such actions highlight XRCC2's role in preserving cell genetic integrity and preventing mutations.

Pathways

Homologous recombination and DNA repair are integral processes where XRCC2 operates. The homologous recombination pathway is critically linked with the maintenance of chromosome integrity during cell division. XRCC2 by cooperating with proteins like RAD51 and BRCA2 forms an essential part of recombinational DNA repair. Its interactions are necessary for orchestrating the repair process ensuring seamless recovery of the genetic code after disruptions.

XRCC2 is associated closely with cancer vulnerabilities due to its role in DNA repair. Deficiencies or mutations in XRCC2 can lead to increased susceptibility to tumors and hereditary breast cancer given its involvement in stabilizing RAD51 function which is pivotal in suppressing tumor formation. The malfunction in XRCC2's DNA repair capabilities can also have connections with Fanconi anemia highlighting XRCC2’s importance to genetic disorders related to impaired DNA repair mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the RAD51 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.

See full target information XRCC2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 13:505 PubMed35643812

2022

PARP inhibitor olaparib enhances the efficacy of radiotherapy on XRCC2-deficient colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Changjiang Qin,Zhiyu Ji,Ertao Zhai,Kaiwu Xu,Yijie Zhang,Quanying Li,Hong Jing,Xiaoliang Wang,Xinming Song

Cell reports 38:110220 PubMed35021081

2022

2-Hydroxyglutarate destabilizes chromatin regulatory landscape and lineage fidelity to promote cellular heterogeneity.

Applications

Unspecified application

Species

Unspecified reactive species

Meena Kusi,Maryam Zand,Li-Ling Lin,Meizhen Chen,Anthony Lopez,Chun-Lin Lin,Chiou-Miin Wang,Nicholas D Lucio,Nameer B Kirma,Jianhua Ruan,Tim H-M Huang,Kohzoh Mitsuya

Journal of Cancer 12:6254-6264 PubMed34539898

2021

Significant value of XRCC2 and XRCC9 expression in the prognosis of human ovarian carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Liu,Yichi Xu,Mengying Jiang,Weinan Chen,Xueqiong Zhu

Cell reports 25:3273-3282.e6 PubMed30566856

2018

XRCC2 Regulates Replication Fork Progression during dNTP Alterations.

Applications

Unspecified application

Species

Unspecified reactive species

Sneha Saxena,Kumar Somyajit,Ganesh Nagaraju

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-XRCC2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-XRCC2 antibody (AB180752)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC2 antibody (AB180752)

Immunocytochemistry/ Immunofluorescence - Anti-XRCC2 antibody (AB180752)

Immunocytochemistry/ Immunofluorescence - Anti-XRCC2 antibody (AB180752)

Western blot - Anti-XRCC2 antibody (AB180752)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise