AB124900

Anti-XRCC2 antibody [EPR5149]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal XRCC2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

DNA repair protein XRCC2, X-ray repair cross-complementing protein 2, XRCC2

2 Images

Flow Cytometry (Intracellular) - Anti-XRCC2 antibody [EPR5149] (AB124900)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-XRCC2 antibody [EPR5149] (AB124900)

ab124900 at 1/100 dilution staining XRCC2 in permeablised HeLa cells by intracellular flow cytometry (red). Rabbit IgG negative control antibody (green).

Unknown

Western blot - Anti-XRCC2 antibody [EPR5149] (AB124900)

All lanes:

Western blot - Anti-XRCC2 antibody [EPR5149] (ab124900) at 1/10000 dilution

Lane 1:

Human testis lysate at 10 µg

Lane 2:

HeLa lysate at 10 µg

Lane 3:

SW480 lysate at 10 µg

Lane 4:

SH-SY5Y lysate at 10 µg

Lane 5:

Caco 2 lysate at 10 µg

Lane 6:

Human kidney lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 32 kDa

false

Carrier free

Anti-XRCC2 antibody [EPR5149] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5149

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

XRCC2 also known as X-ray repair cross-complementing protein 2 is a protein that plays a role in DNA repair mechanisms. It has a molecular mass of approximately 33 kDa. XRCC2 is expressed in various tissues with noticeable abundance in proliferative tissues due to their high demand for DNA repair processes. Mechanistically XRCC2 functions as part of the Rad51 paralog group facilitating homologous recombination repair of DNA double-strand breaks. Its involvement is quintessential for maintaining genomic stability.

Biological function summary

XRCC2 contributes to cellular maintenance by repairing DNA double-strand breaks through the homologous recombination pathway. It participates in the Rad51 paralog complex alongside other paralogs like Rad51B Rad51C Rad51D and XRCC3. This complex ensures precise repair and re-ligation of damaged DNA by assisting in strand exchange and stabilization. Such actions highlight XRCC2's role in preserving cell genetic integrity and preventing mutations.

Pathways

Homologous recombination and DNA repair are integral processes where XRCC2 operates. The homologous recombination pathway is critically linked with the maintenance of chromosome integrity during cell division. XRCC2 by cooperating with proteins like RAD51 and BRCA2 forms an essential part of recombinational DNA repair. Its interactions are necessary for orchestrating the repair process ensuring seamless recovery of the genetic code after disruptions.

XRCC2 is associated closely with cancer vulnerabilities due to its role in DNA repair. Deficiencies or mutations in XRCC2 can lead to increased susceptibility to tumors and hereditary breast cancer given its involvement in stabilizing RAD51 function which is pivotal in suppressing tumor formation. The malfunction in XRCC2's DNA repair capabilities can also have connections with Fanconi anemia highlighting XRCC2’s importance to genetic disorders related to impaired DNA repair mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the RAD51 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.

See full target information XRCC2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 12:1138 PubMed34880209

2021

FDI-6 inhibits the expression and function of FOXM1 to sensitize BRCA-proficient triple-negative breast cancer cells to Olaparib by regulating cell cycle progression and DNA damage repair.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Ping Wang,Shi-Qi Wu,Shi-Hui Huang,Yi-Xuan Tang,Liu-Qiong Meng,Feng Liu,Qi-Hua Zhu,Yun-Gen Xu

Andrology 8:407-420 PubMed31364287

2019

Characterization of seminal plasma proteomic alterations associated with the IVF and rescue-ICSI pregnancy in assisted reproduction.

Applications

Unspecified application

Species

Unspecified reactive species

X Liu,G Liu,P Zhu,Y Wang,J Wang,W Zhang,W Wang,N Li,X Wang,C Zhang,J Liu,X Shen,F Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-XRCC2 antibody [EPR5149]

Flow Cytometry (Intracellular) - Anti-XRCC2 antibody [EPR5149] (AB124900)

Western blot - Anti-XRCC2 antibody [EPR5149] (AB124900)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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