AB321987

Anti-XRCC3 antibody [EPR28957-35] - BSA and Azide free

Recombinant
RabMAb
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Rabbit Recombinant Monoclonal XRCC3 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

DNA repair protein XRCC3, X-ray repair cross-complementing protein 3, XRCC3

2 Images

Supplier Data

Western blot - Anti-XRCC3 antibody [EPR28957-35] - BSA and Azide free (AB321987)

This data was developed using ab321986, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : kidney, liver(PMID : 9660962)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-XRCC3 antibody [EPR28957-35] (<a href='/en-us/products/primary-antibodies/xrcc3-antibody-epr28957-35-ab321986'>ab321986</a>) at 1/1000 dilution

Lane 1:

SK-N-SH (human neuroblastoma epithelial cell) whole cell lysate at 40 µg

Lane 2:

DU 145 (human prostate carcinoma epithelial cell) whole cell lysate at 40 µg

Lane 3:

Human testis tissue lysate at 40 µg

Lane 4:

Human liver tissue lysate at 40 µg

Lane 5:

Human kidney tissue lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 38 kDa,36 kDa

false

Exposure time: 158s

Supplier Data

Western blot - Anti-XRCC3 antibody [EPR28957-35] - BSA and Azide free (AB321987)

This data was developed using ab321986, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-XRCC3 antibody [EPR28957-35] (<a href='/en-us/products/primary-antibodies/xrcc3-antibody-epr28957-35-ab321986'>ab321986</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 40 µg

Lane 2:

HeLa transfected with siRNA specifically targeting XRCC3 whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 38 kDa,36 kDa

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28957-35

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab321987 is the carrier-free version of ab321986.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

XRCC3 also known as X-Ray Repair Cross Complementing 3 plays an important role in DNA repair processes. It is a protein weighing approximately 39 kDa and is expressed in many tissues within the human body. This protein belongs to the RAD51 family of recombinases and is essential during homologous recombination ensuring the stability and integrity of the genome by assisting in the repair of double-strand breaks in DNA.

Biological function summary

XRCC3 promotes DNA repair and genomic stability and functions as part of a complex. It associates with other proteins in the RAD51 family to facilitate the proper repair mechanisms required for maintaining cellular health. XRCC3’s activity in homologous recombination is critical to prevent DNA damage accumulation which could lead to chromosomal abnormalities impacting cell survival and proliferation.

Pathways

XRCC3 is integrally involved in homologous recombination repair and the broader DNA damage response network. It interacts closely with RAD51 and BRCA2 to mediate accurate DNA repair by homologous recombination ensuring precise repair pathway activation and reducing erroneous repair activities. XRCC3 supports the recruitment of RAD51 to DNA breaks and collaborates with the Fanconi anemia pathway to protect against genomic instability.

XRCC3 mutations or dysfunctions have associations with cancer susceptibility particularly breast cancer. The protein’s role in DNA repair links it to other proteins like BRCA1 and BRCA2 which have established connections in hereditary breast and ovarian cancer syndromes. Altered XRCC3 expression or sequence changes can compromise DNA repair pathways heightening the risk of tumorigenesis due to unchecked DNA damage accumulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the RAD51 paralog protein complex CX3 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, CX3 acts downstream of RAD51 recruitment; the complex binds predominantly to the intersection of the four duplex arms of the Holliday junction (HJ) and to junctions of replication forks. Involved in HJ resolution and thus in processing HR intermediates late in the DNA repair process; the function may be linked to the CX3 complex and seems to involve GEN1 during mitotic cell cycle progression. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C.

See full target information DNA repair protein XRCC3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-XRCC3 antibody [EPR28957-35] - BSA and Azide free

Western blot - Anti-XRCC3 antibody [EPR28957-35] - BSA and Azide free (AB321987)

Western blot - Anti-XRCC3 antibody [EPR28957-35] - BSA and Azide free (AB321987)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise