AB20254

Anti-XRCC3 antibody [XRCC3 10F1/6]

Name: Anti-XRCC3 antibody [XRCC3 10F1/6] (ab20254) | Abcam
Brand: Abcam Limited
SKU: AB20254
Price: 535 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(2 Publications)

Mouse Monoclonal XRCC3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Full Length Protein corresponding to Human DNA repair protein XRCC3.

View Alternative Names

DNA repair protein XRCC3, X-ray repair cross-complementing protein 3, XRCC3

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC3 antibody [XRCC3 10F1/6] (AB20254)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC3 antibody [XRCC3 10F1/6] (AB20254)

ab20254 staining human normal placenta. Staining is localized to nuclear compartment.
Left panel : with primary antibody at 4 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

XRCC3 10F1/6

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Full Length Protein corresponding to Human DNA repair protein XRCC3. The exact immunogen used to generate this antibody is proprietary information.

O43542

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

XRCC3 also known as X-Ray Repair Cross Complementing 3 plays an important role in DNA repair processes. It is a protein weighing approximately 39 kDa and is expressed in many tissues within the human body. This protein belongs to the RAD51 family of recombinases and is essential during homologous recombination ensuring the stability and integrity of the genome by assisting in the repair of double-strand breaks in DNA.

Biological function summary

XRCC3 promotes DNA repair and genomic stability and functions as part of a complex. It associates with other proteins in the RAD51 family to facilitate the proper repair mechanisms required for maintaining cellular health. XRCC3’s activity in homologous recombination is critical to prevent DNA damage accumulation which could lead to chromosomal abnormalities impacting cell survival and proliferation.

Pathways

XRCC3 is integrally involved in homologous recombination repair and the broader DNA damage response network. It interacts closely with RAD51 and BRCA2 to mediate accurate DNA repair by homologous recombination ensuring precise repair pathway activation and reducing erroneous repair activities. XRCC3 supports the recruitment of RAD51 to DNA breaks and collaborates with the Fanconi anemia pathway to protect against genomic instability.

XRCC3 mutations or dysfunctions have associations with cancer susceptibility particularly breast cancer. The protein’s role in DNA repair links it to other proteins like BRCA1 and BRCA2 which have established connections in hereditary breast and ovarian cancer syndromes. Altered XRCC3 expression or sequence changes can compromise DNA repair pathways heightening the risk of tumorigenesis due to unchecked DNA damage accumulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the RAD51 paralog protein complex CX3 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, CX3 acts downstream of RAD51 recruitment; the complex binds predominantly to the intersection of the four duplex arms of the Holliday junction (HJ) and to junctions of replication forks. Involved in HJ resolution and thus in processing HR intermediates late in the DNA repair process; the function may be linked to the CX3 complex and seems to involve GEN1 during mitotic cell cycle progression. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C.

See full target information DNA repair protein XRCC3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer biology & therapy 16:1160-71 PubMed26023803

2015

A functional biological network centered on XRCC3: a new possible marker of chemoradiotherapy resistance in rectal cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Marco Agostini,Andrea Zangrando,Chiara Pastrello,Edoardo D'Angelo,Gabriele Romano,Roberto Giovannoni,Marco Giordan,Isacco Maretto,Chiara Bedin,Carlo Zanon,Maura Digito,Giovanni Esposito,Claudia Mescoli,Marialuisa Lavitrano,Flavio Rizzolio,Igor Jurisica,Antonio Giordano,Salvatore Pucciarelli,Donato Nitti

Science signaling 7:ra47 PubMed24847116

2014

Targeting poly(ADP-ribose) polymerase and the c-Myb-regulated DNA damage response pathway in castration-resistant prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Likun Li,Wenjun Chang,Guang Yang,Chengzhen Ren,Sanghee Park,Theodoros Karantanos,Styliani Karanika,Jianxiang Wang,Jianhua Yin,Parantu K Shah,Hirayama Takahiro,Masato Dobashi,Wenling Zhang,Eleni Efstathiou,Sankar N Maity,Ana M Aparicio,Elsa M Li Ning Tapia,Patricia Troncoso,Bradley Broom,Lianchun Xiao,Hyun-Sung Lee,Ju-Seog Lee,Paul G Corn,Nora Navone,Timothy C Thompson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-XRCC3 antibody [XRCC3 10F1/6]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC3 antibody [XRCC3 10F1/6] (AB20254)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise