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Rabbit Polyclonal XRCC4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human DNA repair protein XRCC4 aa 250-300.


Images

Western blot - Anti-XRCC4 antibody (AB97351), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC4 antibody (AB97351), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-XRCC4 antibody (AB97351), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Synthetic Peptide within Human DNA repair protein XRCC4 aa 250-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q13426

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/500.00000 - 1/3000.00000
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100.00000 - 1/1000.00000
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

DNA repair protein XRCC4. DNA non-homologous end joining (NHEJ) core factor, required for double-strand break repair and V(D)J recombination (PubMed:10757784, PubMed:10854421, PubMed:12517771, PubMed:16412978, PubMed:17124166, PubMed:17290226, PubMed:22228831, PubMed:25597996, PubMed:25742519, PubMed:25934149, PubMed:26100018, PubMed:26774286, PubMed:8548796). Acts as a scaffold protein that regulates recruitment of other proteins to DNA double-strand breaks (DSBs) (PubMed:15385968, PubMed:20852255, PubMed:26774286, PubMed:27437582). Associates with NHEJ1/XLF to form alternating helical filaments that bridge DNA and act like a bandage, holding together the broken DNA until it is repaired (PubMed:21768349, PubMed:21775435, PubMed:22287571, PubMed:26100018, PubMed:27437582, PubMed:28500754). The XRCC4-NHEJ1/XLF subcomplex binds to the DNA fragments of a DSB in a highly diffusive manner and robustly bridges two independent DNA molecules, holding the broken DNA fragments in close proximity to one other (PubMed:27437582). The mobility of the bridges ensures that the ends remain accessible for further processing by other repair factors (PubMed:27437582). Plays a key role in the NHEJ ligation step of the broken DNA during DSB repair via direct interaction with DNA ligase IV (LIG4): the LIG4-XRCC4 subcomplex reseals the DNA breaks after the gap filling is completed (PubMed:10757784, PubMed:10854421, PubMed:12517771, PubMed:17290226, PubMed:19837014, PubMed:9242410). XRCC4 stabilizes LIG4, regulates its subcellular localization and enhances LIG4's joining activity (PubMed:10757784, PubMed:10854421, PubMed:12517771, PubMed:17290226, PubMed:21982441, PubMed:22228831, PubMed:9242410). Binding of the LIG4-XRCC4 subcomplex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends (PubMed:10757784, PubMed:10854421). Promotes displacement of PNKP from processed strand break termini (PubMed:20852255, PubMed:28453785). Protein XRCC4, C-terminus. Acts as an activator of the phospholipid scramblase activity of XKR4 (PubMed:33725486). This form, which is generated upon caspase-3 (CASP3) cleavage, translocates into the cytoplasm and interacts with XKR4, thereby promoting phosphatidylserine scramblase activity of XKR4 and leading to phosphatidylserine exposure on apoptotic cell surface (PubMed:33725486).

Alternative names

Recommended products

Rabbit Polyclonal XRCC4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human DNA repair protein XRCC4 aa 250-300.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Synthetic Peptide within Human DNA repair protein XRCC4 aa 250-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q13426
Purification technique
Affinity purification Immunogen
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

XRCC4 also known as X-ray repair cross-complementing protein 4 is an important component in the repair of double-strand breaks in DNA. It stabilizes and facilitates the joining of DNA ends during the non-homologous end joining (NHEJ) repair pathway. XRCC4 has a mass of approximately 38 kDa. This protein is widely expressed in various tissues with significant levels noted in the nucleus reflecting its direct involvement in DNA repair processes.

Biological function summary

XRCC4 plays a pivotal role in maintaining genetic stability. It is a vital component of the DNA repair machinery and functions as part of a complex with DNA ligase IV. This complex ensures the appropriate joining of DNA ends which is critical for cell survival and prevention of chromosomal aberrations. Without XRCC4 cells would face higher mutational rates and increased genomic instability leading to cellular dysfunction and disease progression.

Pathways

XRCC4 is integral to the non-homologous end joining pathway a major DNA double-strand break repair mechanism in human cells. It interacts with DNA-dependent protein kinase (DNA-PK) and DNA ligase IV during the repair process. Within the NHEJ pathway XRCC4 collaborates closely with DNA ligase IV to seal DNA breaks effectively. It also engages with other proteins such as Ku70/Ku80 to ensure proper coordination of DNA repair activities within the cell.

Associated diseases and disorders

XRCC4 stands as an important factor in genomic integrity with its dysfunction associating with cancer and immunodeficiency disorders. Mutations or deficiencies in XRCC4 can lead to a higher incidence of leukemia due to the accumulation of genomic instability. Connections between XRCC4 and the ATM protein have been highlighted in research with ATM being another critical player in DNA repair pathways. Defective XRCC4 or its interactions can exacerbate disease progression making it a significant target for therapeutic interventions.

Product promise

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3 product images

  • Western blot - Anti-XRCC4 antibody (ab97351), expandable thumbnail

    Western blot - Anti-XRCC4 antibody (ab97351)

    10% SDS-PAGE

    All lanes: Western blot - Anti-XRCC4 antibody (ab97351) at 1/500 dilution

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Lane 2: HeLa cell nuclear extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 38 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC4 antibody (ab97351), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC4 antibody (ab97351)

    Immunohistochemical analysis of paraffin-embedded HBL435 xenograft using ab97351 at 1/500 dilution.

    Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

  • Immunocytochemistry/ Immunofluorescence - Anti-XRCC4 antibody (ab97351), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-XRCC4 antibody (ab97351)

    Immunofluorescence analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling XRCC4 using ab97351 at 1/200 dilution. Lower image was also labelled with DNA probe.

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Product protocols

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