AB97351

Anti-XRCC4 antibody

Name: Anti-XRCC4 antibody (ab97351) | Abcam
Brand: Abcam Limited
SKU: AB97351
Rating: 4 (3 reviews)

(3 Reviews)

(7 Publications)

Rabbit Polyclonal XRCC4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human DNA repair protein XRCC4 aa 250-300.

View Alternative Names

DNA repair protein XRCC4, hXRCC4, X-ray repair cross-complementing protein 4, XRCC4

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC4 antibody (AB97351)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC4 antibody (AB97351)

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft using ab97351 at 1/500 dilution.

Antigen Retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunocytochemistry/ Immunofluorescence - Anti-XRCC4 antibody (AB97351)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-XRCC4 antibody (AB97351)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling XRCC4 protein at nucleus using ab97351 at a 1/200 dilution (Green). alpha Tubulin, a cytoskeleton marker, stained by an anti-alpha Tubulin antibody at a 1/1000 dilution (Red).

Supplier Data

Western blot - Anti-XRCC4 antibody (AB97351)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-XRCC4 antibody (ab97351) at 1/1000 dilution

Lane 1:

HeLa whole cell lysates at 30 µg

Lane 2:

HeLa cell membrane lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human DNA repair protein XRCC4 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

Q13426

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

XRCC4 also known as X-ray repair cross-complementing protein 4 is an important component in the repair of double-strand breaks in DNA. It stabilizes and facilitates the joining of DNA ends during the non-homologous end joining (NHEJ) repair pathway. XRCC4 has a mass of approximately 38 kDa. This protein is widely expressed in various tissues with significant levels noted in the nucleus reflecting its direct involvement in DNA repair processes.

Biological function summary

XRCC4 plays a pivotal role in maintaining genetic stability. It is a vital component of the DNA repair machinery and functions as part of a complex with DNA ligase IV. This complex ensures the appropriate joining of DNA ends which is critical for cell survival and prevention of chromosomal aberrations. Without XRCC4 cells would face higher mutational rates and increased genomic instability leading to cellular dysfunction and disease progression.

Pathways

XRCC4 is integral to the non-homologous end joining pathway a major DNA double-strand break repair mechanism in human cells. It interacts with DNA-dependent protein kinase (DNA-PK) and DNA ligase IV during the repair process. Within the NHEJ pathway XRCC4 collaborates closely with DNA ligase IV to seal DNA breaks effectively. It also engages with other proteins such as Ku70/Ku80 to ensure proper coordination of DNA repair activities within the cell.

XRCC4 stands as an important factor in genomic integrity with its dysfunction associating with cancer and immunodeficiency disorders. Mutations or deficiencies in XRCC4 can lead to a higher incidence of leukemia due to the accumulation of genomic instability. Connections between XRCC4 and the ATM protein have been highlighted in research with ATM being another critical player in DNA repair pathways. Defective XRCC4 or its interactions can exacerbate disease progression making it a significant target for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

DNA repair protein XRCC4. DNA non-homologous end joining (NHEJ) core factor, required for double-strand break repair and V(D)J recombination (PubMed : 10757784, PubMed : 10854421, PubMed : 12517771, PubMed : 16412978, PubMed : 17124166, PubMed : 17290226, PubMed : 22228831, PubMed : 25597996, PubMed : 25742519, PubMed : 25934149, PubMed : 26100018, PubMed : 26774286, PubMed : 8548796). Acts as a scaffold protein that regulates recruitment of other proteins to DNA double-strand breaks (DSBs) (PubMed : 15385968, PubMed : 20852255, PubMed : 26774286, PubMed : 27437582). Associates with NHEJ1/XLF to form alternating helical filaments that bridge DNA and act like a bandage, holding together the broken DNA until it is repaired (PubMed : 21768349, PubMed : 21775435, PubMed : 22287571, PubMed : 26100018, PubMed : 27437582, PubMed : 28500754). The XRCC4-NHEJ1/XLF subcomplex binds to the DNA fragments of a DSB in a highly diffusive manner and robustly bridges two independent DNA molecules, holding the broken DNA fragments in close proximity to one other (PubMed : 27437582). The mobility of the bridges ensures that the ends remain accessible for further processing by other repair factors (PubMed : 27437582). Plays a key role in the NHEJ ligation step of the broken DNA during DSB repair via direct interaction with DNA ligase IV (LIG4) : the LIG4-XRCC4 subcomplex reseals the DNA breaks after the gap filling is completed (PubMed : 10757784, PubMed : 10854421, PubMed : 12517771, PubMed : 17290226, PubMed : 19837014, PubMed : 9242410). XRCC4 stabilizes LIG4, regulates its subcellular localization and enhances LIG4's joining activity (PubMed : 10757784, PubMed : 10854421, PubMed : 12517771, PubMed : 17290226, PubMed : 21982441, PubMed : 22228831, PubMed : 9242410). Binding of the LIG4-XRCC4 subcomplex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends (PubMed : 10757784, PubMed : 10854421). Promotes displacement of PNKP from processed strand break termini (PubMed : 20852255, PubMed : 28453785).. Protein XRCC4, C-terminus. Acts as an activator of the phospholipid scramblase activity of XKR4 (PubMed : 33725486). This form, which is generated upon caspase-3 (CASP3) cleavage, translocates into the cytoplasm and interacts with XKR4, thereby promoting phosphatidylserine scramblase activity of XKR4 and leading to phosphatidylserine exposure on apoptotic cell surface (PubMed : 33725486).

See full target information DNA repair protein XRCC4

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6999 PubMed40738898

2025

Tumor-associated neutrophil precursors impair homologous DNA repair and promote sensitivity to PARP inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Siddhartha Mukherjee,Cindy Garda,Letizia Boffa,Angela Rita Elia,Matteo Massara,Maria Teresa Balia,Daniela Brina,Simone Mosole,Anna Campagnari,Giada Andrea Cassanmagnago,Andrea Rinaldi,Giacomo Lazzaroni,David Jarrossay,Diego Morone,Ilaria Ceppi,Riccardo De Sillo,Isabella Giacomini,Ilaria Craparotta,Laura Di Rito,Simon Barry,Endre Laczko,Sebastian Streb,Francesco Meani,Simona Di Lascio,Nancy Hynes,Enrico Lugli,Simone Puccio,Stephen-John Sammut,Ulrike Perriard,Yves Harder,Lorenzo Rossi,Maria Luisa Gasparri,Marco Bolis,Petr Cejka,Arianna Calcinotto

Nucleic acids research 53: PubMed40737094

2025

Late steps of allelic break-induced replication suppress tandem duplication associated with BRCA1 deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Cheng Huang,Yi-Li Feng,Qian Liu,Ruo-Dan Chen,Si-Cheng Liu,Meng Wang,An-Yong Xie

Cell reports 32:108068 PubMed32846126

2020

Functional Radiogenetic Profiling Implicates ERCC6L2 in Non-homologous End Joining.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Francica,Merve Mutlu,Vincent A Blomen,Catarina Oliveira,Zuzanna Nowicka,Anika Trenner,Nora M Gerhards,Peter Bouwman,Elmer Stickel,Maarten L Hekkelman,Lea Lingg,Ismar Klebic,Marieke van de Ven,Renske de Korte-Grimmerink,Denise Howald,Jos Jonkers,Alessandro A Sartori,Wojciech Fendler,J Ross Chapman,Thijn Brummelkamp,Sven Rottenberg

PloS one 13:e0205691 PubMed30352062

2018

Chondrocytes differentiated from human induced pluripotent stem cells: Response to ionizing radiation.

Applications

Unspecified application

Species

Unspecified reactive species

Ewelina Stelcer,Katarzyna Kulcenty,Wiktoria Maria Suchorska

PLoS genetics 14:e1007486 PubMed30024881

2018

Rad51 recruitment and exclusion of non-homologous end joining during homologous recombination at a Tus/Ter mammalian replication fork barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas A Willis,Arvind Panday,Erin E Duffey,Ralph Scully

Cell death discovery 4:10 PubMed29531807

2018

NF-κB inhibition by dimethylaminoparthenolide radiosensitizes non-small-cell lung carcinoma by blocking DNA double-strand break repair.

Applications

Unspecified application

Species

Unspecified reactive species

Peter V Deraska,Colin O'Leary,Hunter D Reavis,Shelby Labe,Tru-Khang Dinh,Jean-Bernard Lazaro,Christopher Sweeney,Alan D D'Andrea,David Kozono

Nature 551:590-595 PubMed29168504

2017

Mechanism of tandem duplication formation in BRCA1-mutant cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas A Willis,Richard L Frock,Francesca Menghi,Erin E Duffey,Arvind Panday,Virginia Camacho,E Paul Hasty,Edison T Liu,Frederick W Alt,Ralph Scully

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-XRCC4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC4 antibody (AB97351)

Immunocytochemistry/ Immunofluorescence - Anti-XRCC4 antibody (AB97351)

Western blot - Anti-XRCC4 antibody (AB97351)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise