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Rabbit Recombinant Monoclonal XRCC4 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.

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Images

Western blot - Anti-XRCC4 antibody [EPR28958-63] - BSA and Azide free (AB317698), expandable thumbnail
  • Western blot - Anti-XRCC4 antibody [EPR28958-63] - BSA and Azide free (AB317698), expandable thumbnail
  • Western blot - Anti-XRCC4 antibody [EPR28958-63] - BSA and Azide free (AB317698), expandable thumbnail
  • Western blot - Anti-XRCC4 antibody [EPR28958-63] - BSA and Azide free (AB317698), expandable thumbnail
  • Immunoprecipitation - Anti-XRCC4 antibody [EPR28958-63] - BSA and Azide free (AB317698), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWB
Human
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

Alternative names

DNA repair protein XRCC4, X-ray repair cross-complementing protein 4, XRCC4

Recommended products

Rabbit Recombinant Monoclonal XRCC4 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.

Alternative names

DNA repair protein XRCC4, X-ray repair cross-complementing protein 4, XRCC4

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR28958-63

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

ab317698 is the carrier-free version of Anti-XRCC4 antibody [EPR28958-63] ab317697.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

XRCC4 also known as X-ray repair cross-complementing protein 4 is an important component in the repair of double-strand breaks in DNA. It stabilizes and facilitates the joining of DNA ends during the non-homologous end joining (NHEJ) repair pathway. XRCC4 has a mass of approximately 38 kDa. This protein is widely expressed in various tissues with significant levels noted in the nucleus reflecting its direct involvement in DNA repair processes.

Biological function summary

XRCC4 plays a pivotal role in maintaining genetic stability. It is a vital component of the DNA repair machinery and functions as part of a complex with DNA ligase IV. This complex ensures the appropriate joining of DNA ends which is critical for cell survival and prevention of chromosomal aberrations. Without XRCC4 cells would face higher mutational rates and increased genomic instability leading to cellular dysfunction and disease progression.

Pathways

XRCC4 is integral to the non-homologous end joining pathway a major DNA double-strand break repair mechanism in human cells. It interacts with DNA-dependent protein kinase (DNA-PK) and DNA ligase IV during the repair process. Within the NHEJ pathway XRCC4 collaborates closely with DNA ligase IV to seal DNA breaks effectively. It also engages with other proteins such as Ku70/Ku80 to ensure proper coordination of DNA repair activities within the cell.

Associated diseases and disorders

XRCC4 stands as an important factor in genomic integrity with its dysfunction associating with cancer and immunodeficiency disorders. Mutations or deficiencies in XRCC4 can lead to a higher incidence of leukemia due to the accumulation of genomic instability. Connections between XRCC4 and the ATM protein have been highlighted in research with ATM being another critical player in DNA repair pathways. Defective XRCC4 or its interactions can exacerbate disease progression making it a significant target for therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-XRCC4 antibody [EPR28958-63] - BSA and Azide free (ab317698), expandable thumbnail

    Western blot - Anti-XRCC4 antibody [EPR28958-63] - BSA and Azide free (ab317698)

    This data was developed using Anti-XRCC4 antibody [EPR28958-63] ab317697, the same antibody clone in a different buffer formulation.

    In Lane 1-Lane 4, lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    All lanes: Western blot - Anti-XRCC4 antibody [EPR28958-63] (Anti-XRCC4 antibody [EPR28958-63] ab317697) at 1/1000 dilution

    Lanes 1 and 6: HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

    Lanes 2 and 5: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lanes 3 and 7: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 4: T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 55 kDa

    Exposure time: 15s

  • Western blot - Anti-XRCC4 antibody [EPR28958-63] - BSA and Azide free (ab317698), expandable thumbnail

    Western blot - Anti-XRCC4 antibody [EPR28958-63] - BSA and Azide free (ab317698)

    This data was developed using Anti-XRCC4 antibody [EPR28958-63] ab317697, the same antibody clone in a different buffer formulation.

    The expression of caspase cleaved XRCC4 is upregulated upon induction of apoptosis (PMID: 33725486).

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-XRCC4 antibody [EPR28958-63] (Anti-XRCC4 antibody [EPR28958-63] ab317697) at 1/1000 dilution

    Lane 1: Untreated heLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HeLa treated with 2uM staurosporine for 3h whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 35 kDa, 55 kDa, 124 kDa

    Exposure time: 15s

    The expression of caspase cleaved XRCC4 is upregulated upon induction of apoptosis (PMID: 33725486).

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

  • Western blot - Anti-XRCC4 antibody [EPR28958-63] - BSA and Azide free (ab317698), expandable thumbnail

    Western blot - Anti-XRCC4 antibody [EPR28958-63] - BSA and Azide free (ab317698)

    This data was developed using Anti-XRCC4 antibody [EPR28958-63] ab317697, the same antibody clone in a different buffer formulation.

    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-XRCC4 antibody [EPR28958-63] (Anti-XRCC4 antibody [EPR28958-63] ab317697) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: HeLa transfected with siRNA specifically targeti XRCC4 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 35 kDa, 55 kDa, 124 kDa

    Exposure time: 48s

    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

  • Western blot - Anti-XRCC4 antibody [EPR28958-63] - BSA and Azide free (ab317698), expandable thumbnail

    Western blot - Anti-XRCC4 antibody [EPR28958-63] - BSA and Azide free (ab317698)

    This data was developed using Anti-XRCC4 antibody [EPR28958-63] ab317697, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-XRCC4 antibody [EPR28958-63] (Anti-XRCC4 antibody [EPR28958-63] ab317697) at 1/1000 dilution

    All lanes: Human tonsil tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 35 kDa, 55 kDa

    Exposure time: 70s

  • Immunoprecipitation - Anti-XRCC4 antibody [EPR28958-63] - BSA and Azide free (ab317698), expandable thumbnail

    Immunoprecipitation - Anti-XRCC4 antibody [EPR28958-63] - BSA and Azide free (ab317698)

    This data was developed using Anti-XRCC4 antibody [EPR28958-63] ab317697, the same antibody clone in a different buffer formulation.

    XRCC4 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with Anti-XRCC4 antibody [EPR28958-63] ab317697 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-XRCC4 antibody [EPR28958-63] ab317697 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 2: Anti-XRCC4 antibody [EPR28958-63] ab317697 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-XRCC4 antibody [EPR28958-63] ab317697 in HeLa whole cell lysate

    All lanes: Immunoprecipitation - Anti-XRCC4 antibody [EPR28958-63] (Anti-XRCC4 antibody [EPR28958-63] ab317697) at 1/30 dilution

    All lanes: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 50s

    XRCC4 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with Anti-XRCC4 antibody [EPR28958-63] ab317697 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-XRCC4 antibody [EPR28958-63] ab317697 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 2: Anti-XRCC4 antibody [EPR28958-63] ab317697 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-XRCC4 antibody [EPR28958-63] ab317697 in HeLa whole cell lysate

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC4 antibody [EPR28958-63] - BSA and Azide free (ab317698), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC4 antibody [EPR28958-63] - BSA and Azide free (ab317698)

    This data was developed using Anti-XRCC4 antibody [EPR28958-63] ab317697, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling XRCC4 with Anti-XRCC4 antibody [EPR28958-63] ab317697 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human breast.

    The section was incubated with Anti-XRCC4 antibody [EPR28958-63] ab317697 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC4 antibody [EPR28958-63] - BSA and Azide free (ab317698), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC4 antibody [EPR28958-63] - BSA and Azide free (ab317698)

    This data was developed using Anti-XRCC4 antibody [EPR28958-63] ab317697, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human endometrioid carcinoma tissue labeling XRCC4 with Anti-XRCC4 antibody [EPR28958-63] ab317697 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human endometrioid carcinoma.

    The section was incubated with Anti-XRCC4 antibody [EPR28958-63] ab317697 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC4 antibody [EPR28958-63] - BSA and Azide free (ab317698), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC4 antibody [EPR28958-63] - BSA and Azide free (ab317698)

    This data was developed using Anti-XRCC4 antibody [EPR28958-63] ab317697, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling XRCC4 with Anti-XRCC4 antibody [EPR28958-63] ab317697 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human tonsil.

    The section was incubated with Anti-XRCC4 antibody [EPR28958-63] ab317697 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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