AB70259

Anti-Xrn1 antibody

Name: Anti-Xrn1 antibody (ab70259) | Abcam
Brand: Abcam Limited
SKU: AB70259
Price: 430 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(10 Publications)

Rabbit Polyclonal XRN1 antibody. Suitable for IP, WB, ICC/IF, EMSA and reacts with Human, Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human XRN1.

View Alternative Names

SEP1, XRN1, 5'-3' exoribonuclease 1, Strand-exchange protein 1 homolog

2 Images

Supplier Data

Immunoprecipitation - Anti-Xrn1 antibody (AB70259)

Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for immunoprecipitation, 20% of IP loaded) and 293T (T; 50 μg) cells. ab70259 used for western blot at 0.1 μg/ml (A) and 1 μg/ml (B) and used for IP at 3 μg/mg lysate. XRN1 was also immunoprecipitated by ab70259. Detection was by chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

All lanes:

Immunoprecipitation - Anti-Xrn1 antibody (ab70259)

Predicted band size: 194 kDa

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Supplier Data

Western blot - Anti-Xrn1 antibody (AB70259)

Detection by chemiluminescence.

All lanes:

Western blot - Anti-Xrn1 antibody (ab70259) at 0.2 µg/mL

Lane 1:

NIH3T3 whole cell lysate at 50 µg

Lane 2:

TCMK-1 whole cell lysate at 50 µg

Lane 3:

4T1 whole cell lysate at 50 µg

Lane 4:

CT26.WT whole cell lysate at 50 µg

Predicted band size: 194 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, EMSA, WB, IP

applications

Immunogen

Synthetic Peptide within Human XRN1. The exact immunogen used to generate this antibody is proprietary information.

Q8IZH2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "EMSA" : {"fullname" : "Electrophoretic Mobility Shift Assay", "shortname":"EMSA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "EMSA-species-checked": "guaranteed", "EMSA-species-dilution-info": "", "EMSA-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/15000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "EMSA-species-checked": "guaranteed", "EMSA-species-dilution-info": "", "EMSA-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "EMSA-species-checked": "predicted", "EMSA-species-dilution-info": "", "EMSA-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "EMSA-species-checked": "predicted", "EMSA-species-dilution-info": "", "EMSA-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "EMSA-species-checked": "predicted", "EMSA-species-dilution-info": "", "EMSA-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "EMSA-species-checked": "predicted", "EMSA-species-dilution-info": "", "EMSA-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "EMSA-species-checked": "predicted", "EMSA-species-dilution-info": "", "EMSA-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "EMSA-species-checked": "predicted", "EMSA-species-dilution-info": "", "EMSA-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Xrn1 also known as 5'-3' Exoribonuclease 1 plays an important mechanical role in degrading RNA molecules. It is an enzyme with a molecular mass of approximately 212 kDa. Xrn1 is expressed in a wide range of eukaryotic organisms where it functions in the cytoplasm. It processes RNA molecules by degrading them from the 5' to the 3' end effectively participating in RNA turnover and quality control mechanisms. This activity highlights Xrn1's importance in cellular RNA regulation.

Biological function summary

The role of Xrn1 is fundamental in maintaining RNA stability and synthesis within eukaryotic cells. Xrn1 does not typically form part of a larger protein complex but rather operates independently in the cytoplasm. Its enzymatic function assists in removing defective redundant or obsolete RNAs ensuring that cells allocate resources only to functional and necessary RNA transcripts. This degradation process directly impacts mRNA turnover securing proper gene expression regulation.

Pathways

The enzymatic action of Xrn1 fits into the mRNA decay pathways notably the nonsense-mediated mRNA decay (NMD) and major mRNA degradation pathway. During NMD Xrn1 works alongside other proteins such as Upf1 which identifies faulty mRNAs for degradation. These pathways ensure that the genetic information is accurately translated into proteins by removing erroneous or incomplete transcripts. Xrn1's activity aligns with its critical involvement in maintaining cellular homeostasis through these pathways.

Alterations in Xrn1 function relate to conditions like cancer and neurological disorders. Misregulation of Xrn1 activity can lead to abnormal RNA accumulation contributing to uncontrolled cell proliferation in cancer. Additionally connections exist between Xrn1 and the protein TDP-43 particularly in neurodegenerative disorders where improper RNA processing can result in toxic RNA species accumulation. Studying Xrn1 function and its interactive partners could advance understanding and potential treatment approaches for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Major 5'-3' exoribonuclease involved in mRNA decay. Required for the 5'-3'-processing of the G4 tetraplex-containing DNA and RNA substrates. The kinetic of hydrolysis is faster for G4 RNA tetraplex than for G4 DNA tetraplex and monomeric RNA tetraplex. Binds to RNA and DNA (By similarity). Plays a role in replication-dependent histone mRNA degradation. May act as a tumor suppressor protein in osteogenic sarcoma (OGS).

See full target information XRN1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 95:e0033621 PubMed33853967

2021

Feline Calicivirus Proteinase-Polymerase Protein Degrades mRNAs To Inhibit Host Gene Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Hongxia Wu,Jiapei Huang,Yongxiang Liu,Yudi Pan,Yin Li,Qian Miao,Liandong Qu,Jin Tian

Redox biology 38:101801 PubMed33232910

2020

The mA reader YTHDC2 inhibits lung adenocarcinoma tumorigenesis by suppressing SLC7A11-dependent antioxidant function.

Applications

Unspecified application

Species

Unspecified reactive species

Lifang Ma,Tianxiang Chen,Xiao Zhang,Yayou Miao,Xiaoting Tian,Keke Yu,Xin Xu,Yongjie Niu,Susu Guo,Congcong Zhang,Shiyu Qiu,Yongxia Qiao,Wentao Fang,Lutao Du,Yongchun Yu,Jiayi Wang

Nucleic acids research 48:9872-9885 PubMed32890404

2020

Ribosomal stalk proteins RPLP1 and RPLP2 promote biogenesis of flaviviral and cellular multi-pass transmembrane proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael K Campos,H R Sagara Wijeratne,Premal Shah,Mariano A Garcia-Blanco,Shelton S Bradrick

Journal of leukocyte biology 109:593-603 PubMed32829531

2020

Regulation of RNA degradation pathways during the lipopolysaccharide response in Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Chi Lai,Alexander James,John Luff,Paul De Souza,Hazel Quek,Uda Ho,Martin F Lavin,Tara L Roberts

Cell reports 29:4422-4434.e13 PubMed31875550

2019

Decapping Enzyme NUDT12 Partners with BLMH for Cytoplasmic Surveillance of NAD-Capped RNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wu,Lingyun Li,Kuan-Ming Chen,David Homolka,Pascal Gos,Fabienne Fleury-Olela,Andrew A McCarthy,Ramesh S Pillai

JCI insight 4: PubMed30721151

2019

Epigenetic modulation of β cells by interferon-α via PNPT1/mir-26a/TET2 triggers autoimmune diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Mihaela Stefan-Lifshitz,Esra Karakose,Lingguang Cui,Abora Ettela,Zhengzi Yi,Weijia Zhang,Yaron Tomer

Molecular cell 68:615-625.e9 PubMed29056324

2017

The Dynamics of mRNA Turnover Revealed by Single-Molecule Imaging in Single Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ivana Horvathova,Franka Voigt,Anna V Kotrys,Yinxiu Zhan,Caroline G Artus-Revel,Jan Eglinger,Michael B Stadler,Luca Giorgetti,Jeffrey A Chao

Molecular cell 68:374-387.e12 PubMed29033321

2017

Regulation of mA Transcripts by the 3'→5' RNA Helicase YTHDC2 Is Essential for a Successful Meiotic Program in the Mammalian Germline.

Applications

Unspecified application

Species

Unspecified reactive species

Magdalena Natalia Wojtas,Radha Raman Pandey,Mateusz Mendel,David Homolka,Ravi Sachidanandam,Ramesh S Pillai

PloS one 9:e112742 PubMed25409157

2014

UVC-induced stress granules in mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed Taha Moutaoufik,Rachid El Fatimy,Hassan Nassour,Cristina Gareau,Jérôme Lang,Robert M Tanguay,Rachid Mazroui,Edouard W Khandjian

Biochemical and biophysical research communication 400:46-52 PubMed20691152

2010

Tristetraprolin controls the stability of cIAP2 mRNA through binding to the 3'UTR of cIAP2 mRNA.

Applications

EMSA

Species

Human

Chae Won Kim,Hong Kyeung Kim,Mai-Tram Vo,Hyun Hee Lee,Hyo Jeong Kim,Young Joo Min,Wha Ja Cho,Jeong Woo Park

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Xrn1 antibody

Immunoprecipitation - Anti-Xrn1 antibody (AB70259)

Western blot - Anti-Xrn1 antibody (AB70259)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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