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Rabbit Recombinant Monoclonal XRN2 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra), ICC/IF and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] (AB318132), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] (AB318132), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] (AB318132), expandable thumbnail
  • Western blot - Anti-XRN2 antibody [EPR28598-13] (AB318132), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] (AB318132), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBFlow Cyt (Intra)ICC/IFIP
Human
Tested
Tested
Tested
Tested
Not recommended
Mouse
Tested
Tested
Expected
Expected
Not recommended
Rat
Tested
Tested
Expected
Expected
Not recommended

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    Possesses 5'->3' exoribonuclease activity (By similarity). May promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription. Binds to RNA polymerase II (RNAp II) transcription termination R-loops formed by G-rich pause sites (PubMed:21700224).

    Alternative names

    5'-3' exoribonuclease 2, DHM1-like protein, DHP protein, XRN2

    Recommended products

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    Rabbit Recombinant Monoclonal XRN2 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra), ICC/IF and reacts with Human, Mouse, Rat samples.

    Alternative names

    5'-3' exoribonuclease 2, DHM1-like protein, DHP protein, XRN2

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Clone number

    EPR28598-13

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate short-term storage duration

    1-2 weeks

    Appropriate short-term storage conditions

    +4°C

    Appropriate long-term storage conditions

    -20°C

    Aliquoting information

    Upon delivery aliquot

    Storage information

    Avoid freeze / thaw cycle

    Notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Supplementary info

    Activity summary

    XRN2 also known as 5'-3' exoribonuclease 2 is an enzyme involved in RNA metabolism. It has a molecular mass of approximately 105 kDa. XRN2 scavenges for the degradation of uncapped nascent RNA by cleaving RNA in the 5' to 3' direction after exonucleolytic cleavage. Predominantly expressed in the nucleus XRN2 plays an important role in RNA turnover and processing contributing to the precise control of gene expression within eukaryotic cells.

    Biological function summary

    5'-3' exoribonuclease 2 regulates the termination of transcription facilitating the completion of the RNA synthesis process. XRN2 functions in coordination with other components within transcription termination complexes such as the exosome. These complexes ensure the removal of excess or faulty RNA which is critical for maintaining cellular homeostasis. XRN2's activity aids in silencing transcriptional noise and preventing the accumulation of defective RNA species.

    Pathways

    5'-3' RNA decay and termination are where XRN2 finds its primary function. It is a part of the exosome-mediated RNA decay pathway and contributes to effective gene expression pathways. In these pathways XRN2 functions alongside proteins such as Rrp6 and other cofactors ensuring the fidelity of RNA processing is maintained. It interacts with proteins involved in RNA polymerase II-mediated transcriptional processes highlighting its integral role in gene expression regulation.

    Associated diseases and disorders

    Improper function or expression of XRN2 is linked to cancer and neurodegenerative diseases. In cancer alterations in RNA processing pathways involving XRN2 can lead to unchecked cellular proliferation. Additionally proteins like SEPT7 are sometimes altered in these pathways contributing to oncogenic potentials. In neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS) disrupted XRN2 function can result in abnormal RNA processing and neuronal damage. Here interactions with proteins like TDP-43 can exacerbate disease pathology linking XRN2 to complex disease mechanisms.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    9 product images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] (ab318132), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] (ab318132)

      Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling XRN2 with ab318132 at 1/1000 (0.501 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining in human cerebrum.
      The section was incubated with ab318132 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] (ab318132), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] (ab318132)

      Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling XRN2 with ab318132 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining in mouse lung.
      The section was incubated with ab318132 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] (ab318132), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] (ab318132)

      Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling XRN2 with ab318132 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining in rat cerebrum.
      The section was incubated with ab318132 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Western blot - Anti-XRN2 antibody [EPR28598-13] (ab318132), expandable thumbnail

      Western blot - Anti-XRN2 antibody [EPR28598-13] (ab318132)

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

      All lanes: Western blot - Anti-XRN2 antibody [EPR28598-13] (ab318132) at 1/1000 dilution

      Lane 1: Human cerebellum tissue lysate at 20 µg

      Lane 2: Human liver tissue lysate at 20 µg

      Secondary

      All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/5000 dilution

      Observed band size: 108 kDa, 36 kDa

      Exposure time: 180s

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] (ab318132), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] (ab318132)

      Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling XRN2 with ab318132 at 1/1000 (0.501 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining in human lung.
      The section was incubated with ab318132 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunocytochemistry/ Immunofluorescence - Anti-XRN2 antibody [EPR28598-13] (ab318132), expandable thumbnail

      Immunocytochemistry/ Immunofluorescence - Anti-XRN2 antibody [EPR28598-13] (ab318132)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling XRN2 with ab318132 at 1/100 (5.01 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

      Confocal image showing nuclear staining in HeLa cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

    • Western blot - Anti-XRN2 antibody [EPR28598-13] (ab318132), expandable thumbnail

      Western blot - Anti-XRN2 antibody [EPR28598-13] (ab318132)

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

      All lanes: Western blot - Anti-XRN2 antibody [EPR28598-13] (ab318132) at 1/1000 dilution

      Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

      Lane 2: HeLa transfected with siRNA specifically targeting XRN2 whole cell lysate at 20 µg

      Lane 3: LN-229 (human brain glioblastoma epithelial cell) whole cell lysate at 20 µg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

      Observed band size: 108 kDa, 36 kDa

      Exposure time: 8s

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] (ab318132), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] (ab318132)

      Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling XRN2 with ab318132 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining in mouse cerebrum.
      The section was incubated with ab318132 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Flow Cytometry (Intracellular) - Anti-XRN2 antibody [EPR28598-13] (ab318132), expandable thumbnail

      Flow Cytometry (Intracellular) - Anti-XRN2 antibody [EPR28598-13] (ab318132)

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling XRN2 with ab318132 at 1/500 dilution (0.1 ug)/Magenta (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

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