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AB254831

Anti-XYLB antibody

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(1 Publication)

Rabbit Polyclonal XYLB antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human XYLB aa 300-450.

View Alternative Names

Xylulose kinase, Xylulokinase, XYLB

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XYLB antibody (AB254831)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XYLB antibody (AB254831)

Paraffin-embedded human stomach tissue stained for XYLB using ab254831 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-XYLB antibody (AB254831)
  • WB

Supplier Data

Western blot - Anti-XYLB antibody (AB254831)

All lanes:

Western blot - Anti-XYLB antibody (ab254831) at 0.4 µg/mL

Lane 1:

NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II (Rat bladder carcinoma cell line) cell lysate

Predicted band size: 58 kDa

false

Western blot - Anti-XYLB antibody (AB254831)
  • WB

Supplier Data

Western blot - Anti-XYLB antibody (AB254831)

Lane 1:

Western blot - Anti-XYLB antibody (ab254831)

Lane 2:

Western blot - Anti-XYLB antibody (ab254831) at 0.4 µg/mL

Lane 1:

MW Ladder

Lane 2:

RT4 (Human urinary bladder cancer cell line) cell lysate

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human XYLB aa 300-450. The exact immunogen used to generate this antibody is proprietary information.

O75191

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The XYLB protein also known as xylulose kinase plays a significant mechanical role in carbohydrate metabolism. It is a 57 kDa enzyme responsible for catalyzing the phosphorylation of xylulose a five-carbon sugar into xylulose-5-phosphate. This activity is important in the pentose phosphate pathway and is highly expressed in the liver kidney and intestine tissues where glucose and fructose metabolism primarily occur.
Biological function summary

Xylulose kinase contributes to the interconversion of carbohydrate molecules facilitating the utilization of xylose a sugar derived from plant biomass. While it does not directly function as part of a larger macromolecular complex XYLB supports the transformation of xylulose as part of the broader carbohydrate metabolic processes. It helps maintain a balance in energy metabolism and influences the flux of metabolites through various pathways in cells.

Pathways

Xylulose kinase is an important enzyme in the pentose phosphate pathway a pathway critical for nucleotide biosynthesis and antioxidant defense. This pathway interacts with glycolysis and is closely related to enzymes like transketolase and aldolase which further process sugar phosphates into energy-rich intermediates. XYLB's activity helps replenish metabolites into the glycolytic pathway connecting it to glucose metabolism and cellular respiration processes.

Alterations in XYLB activity have implications in metabolic disorders such as diabetes and obesity. Disrupted xylose metabolism can contribute to imbalances in sugar processing and energy utilization potentially exacerbating symptoms of these conditions. Research indicates a potential interaction between XYLB and glucose-6-phosphate dehydrogenase an enzyme whose dysfunction is relevant in metabolic syndrome highlighting bxyulose kinase's role in systemic carbohydrate management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphorylates D-xylulose to produce D-xylulose 5-phosphate, a molecule that may play an important role in the regulation of glucose metabolism and lipogenesis.
See full target information XYLB
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncogenesis 11:19 PubMed35459265

2022

TCRP1 activated by mutant p53 promotes NSCLC proliferation via inhibiting FOXO3a.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Liu,Xiaoting Jia,Kai Luo,Xiangzhou Chen,Zhijie Zhang,Danyang Chen,Yixue Gu,Zhimin He,Guopei Zheng
View all publications
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Product promise

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