Rat Monoclonal xyloglucan antibody. Suitable for ICC/IF and reacts with Tobacco, Plants samples. Cited in 3 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-xyloglucan antibody [LM15] (AB190146), expandable thumbnail](https://content.abcam.com/products/images/xyloglucan-antibody-lm15-ab190146--immunocytochemistry-immunofluorescence-img107053.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-xyloglucan antibody [LM15] (AB190146)")
Publications
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- Plant direct 7:e4822023endo-glucanase 16 localizes to the cell walls of developing tissues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHila Behar,Yaseen Mottiar,Rohan Chandrasekhar,Allegra Corelli Grappadelli,Markus Pauly,A Lacey Samuels,Shawn D Mansfield,Harry BrumerPubMed 36733272
- Physiologia plantarum 174:e136832022Physcomitrium (Physcomitrella) patens endo-glucanase 16 is involved in the cell wall development of young tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHila Behar,Anne Lacey Samuels,Harry BrumerPubMed 35396710
- BMC plant biology 8:602008Pectic homogalacturonan masks abundant sets of xyloglucan epitopes in plant cell walls.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSusan E Marcus et. al.PubMed 18498625
Key facts
- Isotype
- IgG2c
- Host species
- Rat
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Arabidopsis suecica | Predicted |
| Arabidopsis thaliana | Predicted |
| Arachis hypogaea | Predicted |
| Plants | Expected |
| Tobacco | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tobacco | Dilution info 3 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Plants | Dilution info 3 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Arabidopsis thaliana, Arabidopsis suecica, Arachis hypogaea | Dilution info - | Notes - |
Target data
Recommended products
Rat Monoclonal xyloglucan antibody. Suitable for ICC/IF and reacts with Tobacco, Plants samples. Cited in 3 publications.
Key facts
- Isotype
- IgG2c
- Host species
- Rat
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- LM15
- Purification technique
- Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunocytochemistry/ Immunofluorescence - Anti-xyloglucan antibody [LM15] (ab190146), expandable thumbnail](https://content.abcam.com/products/images/xyloglucan-antibody-lm15-ab190146--immunocytochemistry-immunofluorescence-img107053.jpg "Immunocytochemistry/ Immunofluorescence - Anti-xyloglucan antibody [LM15] (ab190146)")
Immunocytochemistry/ Immunofluorescence - Anti-xyloglucan antibody [LM15] (ab190146)
ab190146 staining Xyloglucan in 10μm thick tobacco plant stem internode sections, after 6 weeks of growth, fixed in 4% formaldehyde.
Sections were treated with and without pectate lysate (to remove pectic homogalacturonan and further expose the LM15 epitope). Non-specific protein-protein interactions were blocked in 3% BSA/0.3M glycine in 0.1%PBS-Tween for 1h. The sections were then incubated with ab190146 at 3μg/ml, for 2h at room temperature. Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed ab150165, Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed, was used as the secondary antibody at 2μg/ml, for 1h at room temperature (shown in green) As expected, the staining is stronger in pectate lysate treated sections than in untreated.
Also included are negative control images (secondary antibody only) for both treatments, demonstrating antibody specificity and low levels of auto-fluorescence.
Downloads
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