AB197193

Anti-XYLT1 antibody

Name: Anti-XYLT1 antibody (ab197193) | Abcam
Brand: Abcam Limited
SKU: AB197193
Price: 510 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal XYLT1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human XYLT1.

View Alternative Names

XT1, XYLT1, Xylosyltransferase 1, Peptide O-xylosyltransferase 1, Xylosyltransferase I, XT-I, XylT-I

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XYLT1 antibody (AB197193)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XYLT1 antibody (AB197193)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human fetal muscle tissue, labeling XYLT1 with ab197193 at 1/100 dilution.

Supplier Data

Western blot - Anti-XYLT1 antibody (AB197193)

All lanes:

Western blot - Anti-XYLT1 antibody (ab197193) at 1/500 dilution

All lanes:

293 cell lysate

Predicted band size: 108 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human XYLT1. The exact immunogen used to generate this antibody is proprietary information.

Q86Y38

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Lyophilized

Reconstitution

reconstitute with water at 200µL

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as XYLT1 also referred to as Xylosyltransferase 1 is an enzyme with a molecular mass of approximately 95 kDa. It functions mechanically to initiate the glycosaminoglycan (GAG) biosynthesis by transferring xylose from UDP-xylose to serine residues in the core protein of proteoglycans. Expression of XYLT1 occurs broadly in various tissues with higher levels noted in connective tissues such as cartilage tendon and skin where it plays a significant role in the structural integrity and cellular processes.

Biological function summary

The enzyme catalyzes a critical step in proteoglycan biosynthesis which is essential for assembling the extracellular matrix. This process contributes to the formation of glycoproteins and the maintenance of cell signaling pathways. As part of the xylosyltransferase family XYLT1 works in conjunction with other enzymes to ensure the proper addition of sugar chains during proteoglycan synthesis. By carrying out its function XYLT1 supports the development and repair of connective tissues.

Pathways

XYLT1 engages in the glycosaminoglycan biosynthesis and proteoglycan synthesis pathways. It interacts with proteins such as aggrecan and decorin which are key components in cartilage structure and function. The activity of XYLT1 serves as a precursor step in these pathways as successful GAG chain initiation is mandatory for further enzymatic actions such as those catalyzed by enzymes like chondroitin polymerizing factor.

The disruption of XYLT1 functionality can lead to conditions such as pseudoachondroplasia and spondyloepiphyseal dysplasia both of which affect bone growth and cartilage function. XYLT1 mutations can compromise proteoglycan synthesis thereby impacting the extracellular matrix structure. Such mutations may also influence the activity of other related proteins like COMP (Cartilage Oligomeric Matrix Protein) which is linked to these skeletal disorders. Understanding the role of XYLT1 helps in the diagnosis and potential therapeutic approaches for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the first step in the biosynthesis of chondroitin sulfate and dermatan sulfate proteoglycans, such as DCN. Transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein (PubMed : 15461586, PubMed : 17189265, PubMed : 23982343, PubMed : 24581741). Required for normal embryonic and postnatal skeleton development, especially of the long bones (PubMed : 23982343, PubMed : 24581741). Required for normal maturation of chondrocytes during bone development, and normal onset of ossification (By similarity).

See full target information XYLT1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational research : the journal of laboratory and clinical medicine 259:35-45 PubMed37085047

2023

Diabetes mellitus and aortic stenosis head to head: toward personalized medicine in patients with both pathologies.

Applications

Unspecified application

Species

Unspecified reactive species

Nerea Corbacho-Alonso,Tamara Sastre-Oliva,Luis F López-Almodovar,Jorge Solis,Luis R Padial,Teresa Tejerina,Montserrat Carrascal,Laura Mourino-Alvarez,Maria G Barderas

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-XYLT1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XYLT1 antibody (AB197193)

Western blot - Anti-XYLT1 antibody (AB197193)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Reconstitution

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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