Rabbit Polyclonal XYLT2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human XYLT2 aa 250-550.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human XYLT2 aa 250-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H1B5
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Dog | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Dog | Dilution info - | Notes - |
Target data
Function
Catalyzes the first step in the biosynthesis of chondroitin sulfate, heparan sulfate and dermatan sulfate proteoglycans, such as DCN. Transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein.
Alternative names
XT2, UNQ3058/PRO9878, XYLT2, Xylosyltransferase 2, Peptide O-xylosyltransferase 1, Xylosyltransferase II, XT-II, XylT-II
Recommended products
Rabbit Polyclonal XYLT2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human XYLT2 aa 250-550.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human XYLT2 aa 250-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H1B5
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
XYLT2 also known as xylosyltransferase 2 is an enzyme important in the glycosylation process with a molecular weight of approximately 95 kDa. This protein is mainly found in the Golgi apparatus where it participates in the attachment of xylose to specific serine residues within the core protein aiding in the initiation of glycosaminoglycan (GAG) chains. XYLT2's activity contributes to the construction and maintenance of proteoglycans which have a structural and functional role in various cellular matrices.
- Biological function summary
XYLT2 operates as an important enzyme in the biosynthesis of glycosaminoglycans primarily contributing to the formation of chondroitin sulfate and heparan sulfate chains. These chains attach to proteoglycans which become part of the extracellular matrix. It doesn’t work as part of a large complex but closely aligns with other enzymes involved in the elongation and modification of GAG chains. The enzyme's activity ensures the proper synthesis and regulation of these macromolecules affecting cellular communication and structural integrity.
- Pathways
XYLT2 significantly impacts the GAG biosynthetic pathway. It interacts with proteins like aggrecan and versican which are foundational components of the extracellular matrix. The heparan sulfate biosynthesis pathway also incorporates XYLT2 influencing cellular adhesion and signaling. These pathways play important roles in tissue development and repair as well as in maintaining homeostasis in connective tissues.
- Associated diseases and disorders
XYLT2's activity has connections to disorders such as Ehlers-Danlos syndrome and hereditary multiple exostoses. Mutations or altered expression levels in the XYLT2 gene can lead to abnormal GAG chain formation contributing to these conditions. This relationship involves disturbances in other proteins like EXT1 and EXT2 implicated in cartilage development and maintenance. Understanding XYLT2's role in these disorders can help in diagnosing and potentially developing targeted therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-XYLT2 antibody (ab155193)
7.5% SDS PAGE
All lanes: Western blot - Anti-XYLT2 antibody (ab155193) at 1/1000 dilution
All lanes: HeLa whole cell lysate at 30 µg
Predicted band size: 97 kDa
Immunocytochemistry/ Immunofluorescence - Anti-XYLT2 antibody (ab155193)
Immunofluorescent analysis of methanol-fixed HeLa cells labeling XYLT2 with ab155193 at 1/500 dilution (green). Alpha tubulin filaments were labeled red.
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