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AB155193

Anti-XYLT2 antibody

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(1 Publication)

Rabbit Polyclonal XYLT2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human XYLT2 aa 250-550.

View Alternative Names

XT2, UNQ3058/PRO9878, XYLT2, Xylosyltransferase 2, Peptide O-xylosyltransferase 1, Xylosyltransferase II, XT-II, XylT-II

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-XYLT2 antibody (AB155193)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-XYLT2 antibody (AB155193)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling XYLT2 with ab155193 at 1/500 dilution (green). Alpha tubulin filaments were labeled red.

Western blot - Anti-XYLT2 antibody (AB155193)
  • WB

Unknown

Western blot - Anti-XYLT2 antibody (AB155193)

7.5% SDS PAGE

All lanes:

Western blot - Anti-XYLT2 antibody (ab155193) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 97 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human XYLT2 aa 250-550. The exact immunogen used to generate this antibody is proprietary information.

Q9H1B5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

XYLT2 also known as xylosyltransferase 2 is an enzyme important in the glycosylation process with a molecular weight of approximately 95 kDa. This protein is mainly found in the Golgi apparatus where it participates in the attachment of xylose to specific serine residues within the core protein aiding in the initiation of glycosaminoglycan (GAG) chains. XYLT2's activity contributes to the construction and maintenance of proteoglycans which have a structural and functional role in various cellular matrices.
Biological function summary

XYLT2 operates as an important enzyme in the biosynthesis of glycosaminoglycans primarily contributing to the formation of chondroitin sulfate and heparan sulfate chains. These chains attach to proteoglycans which become part of the extracellular matrix. It doesn’t work as part of a large complex but closely aligns with other enzymes involved in the elongation and modification of GAG chains. The enzyme's activity ensures the proper synthesis and regulation of these macromolecules affecting cellular communication and structural integrity.

Pathways

XYLT2 significantly impacts the GAG biosynthetic pathway. It interacts with proteins like aggrecan and versican which are foundational components of the extracellular matrix. The heparan sulfate biosynthesis pathway also incorporates XYLT2 influencing cellular adhesion and signaling. These pathways play important roles in tissue development and repair as well as in maintaining homeostasis in connective tissues.

XYLT2's activity has connections to disorders such as Ehlers-Danlos syndrome and hereditary multiple exostoses. Mutations or altered expression levels in the XYLT2 gene can lead to abnormal GAG chain formation contributing to these conditions. This relationship involves disturbances in other proteins like EXT1 and EXT2 implicated in cartilage development and maintenance. Understanding XYLT2's role in these disorders can help in diagnosing and potentially developing targeted therapies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the first step in the biosynthesis of chondroitin sulfate, heparan sulfate and dermatan sulfate proteoglycans, such as DCN. Transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein.
See full target information XYLT2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell 166:1295-1307.e21 PubMed27565350

2016

Proteomic Analysis of Unbounded Cellular Compartments: Synaptic Clefts.

Applications

Unspecified application

Species

Unspecified reactive species

Ken H Loh,Philipp S Stawski,Austin S Draycott,Namrata D Udeshi,Emily K Lehrman,Daniel K Wilton,Tanya Svinkina,Thomas J Deerinck,Mark H Ellisman,Beth Stevens,Steven A Carr,Alice Y Ting
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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