Rabbit Polyclonal Y14 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RBM8A.
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- Experimental biology and medicine (Maywood, N.J.) 248:217-2312023Weighted gene co-expression network analysis reveals the hub genes associated with pulmonary hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengyan Wang,Dejun Sun,Chuanchuan Liu,Yong Guo,Jie Ma,Ri-Li Ge,Sen CuiPubMed 36740764
- Journal of virology 92:2018Epstein-Barr Virus-Induced Nodules on Viral Replication Compartments Contain RNA Processing Proteins and a Viral Long Noncoding RNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRichard Park et. al.PubMed 30068640
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RBM8A. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5S9
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Expected | Tested | Expected |
| Cow | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted |
| Zebrafish | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Xenopus laevis, Zebrafish | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Xenopus laevis, Zebrafish | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Xenopus laevis, Zebrafish | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Required for pre-mRNA splicing as component of the spliceosome (PubMed:28502770, PubMed:29301961). Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. The MAGOH-RBM8A heterodimer is a component of the nonsense mediated decay (NMD) pathway. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly.
Alternative names
RBM8, HSPC114, MDS014, RBM8A, RNA-binding protein 8A, Binder of OVCA1-1, RNA-binding motif protein 8A, RNA-binding protein Y14, Ribonucleoprotein RBM8A, BOV-1
Recommended products
Rabbit Polyclonal Y14 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RBM8A.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RBM8A. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5S9
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Y14 also known as RBM8A is a protein integral to the exon junction complex (EJC) with an approximate mass of 18 kDa. It localizes mainly in the nucleus of cells. Y14 works with other proteins such as MAGOH serving a role in post-splicing mRNA regulation. Across various tissues Y14 shows expression but is particularly notable in tissues with high levels of mRNA processing like the brain and testis.
- Biological function summary
Y14 functions in mRNA surveillance and quality control mechanisms. It forms part of the exon junction complex a multi-protein structure deposited on mRNA after splicing occurs. The component acts importantly in nonsense-mediated mRNA decay (NMD) a process that ensures defective mRNAs do not translate into dysfunctional proteins. Y14 contributes significantly to mRNA export from the nucleus binding to spliced mRNA and guiding them towards translation sites in the cytoplasm.
- Pathways
Y14 plays a significant role in the nonsense-mediated mRNA decay pathway working in tandem with proteins such as UPF1 and UPF3. This pathway helps identify and degrade mRNA transcripts with premature stop codons therefore protecting cells from expressing truncated proteins that may disrupt normal cellular functions. Moreover Y14 is linked to the mRNA export pathway ensuring that only properly spliced messages reach the ribosome for translation.
- Associated diseases and disorders
Y14 relevance appears in relation to neurodevelopmental disorders and certain cancers. Mutations or dysregulation of Y14 might contribute to conditions such as intellectual disability due to its involvement in mRNA surveillance that affects neuronal development. Additionally aberrant expression of Y14 has connections to breast cancer where it interacts with proteins involved in altered signaling pathways impacting cancer progression and metastasis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-Y14 antibody (ab229573)
15% SDS-PAGE gel.
All lanes: Western blot - Anti-Y14 antibody (ab229573) at 1/1000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg
Lane 2: A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg
Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg
Predicted band size: 19 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Y14 antibody (ab229573)
Paraffin-embedded rat kidney tissue stained for Y14 using ab229573 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Y14 antibody (ab229573)
Paraffin-embedded rat adrenal gland tissue stained for Y14 using ab229573 at 1/500 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-Y14 antibody (ab229573)
4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Y14 (green) using ab229573 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
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