AB177945

Anti-YAF2 antibody [EPR11020]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal YAF2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

YY1-associated factor 2, YAF2

3 Images

Lab

Western blot - Anti-YAF2 antibody [EPR11020] (AB177945)

Blocking and diluting buffer : 5% NFDM/TBST.

Lane 1 : 3 seconds

Lane 2-6 : 180 seconds

Negative sample : human kidney, human pancreas (PMID : 11953439).

This antibody is not suitable to detect YAF2 in cell lines.

All lanes:

Western blot - Anti-YAF2 antibody [EPR11020] (ab177945) at 1/1000 dilution

Lane 1:

Human heart lysate at 20 µg

Lane 2:

Human kidney lysate at 20 µg

Lane 3:

Human pancreas lysate at 20 µg

Lane 4:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

HEK293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 6:

HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 20 kDa

Observed band size: 20 kDa

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Supplier Data

Immunoprecipitation - Anti-YAF2 antibody [EPR11020] (AB177945)

Western blot analysis on immunoprecipitation pellet from (1) Human skeletal muscle lysate or (2) 1X PBS (negative control) using ab177945 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-YAF2 antibody [EPR11020] (ab177945)

Predicted band size: 20 kDa

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Supplier Data

Western blot - Anti-YAF2 antibody [EPR11020] (AB177945)

All lanes:

Western blot - Anti-YAF2 antibody [EPR11020] (ab177945) at 1/1000 dilution

Lane 1:

Human skeletal muscle lysate at 10 µg

Lane 2:

Fetal heart lysate at 10 µg

Predicted band size: 20 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11020

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is not suitable to detect YAF2 in cell lines.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

YAF2 also known as YY1AP1 is a protein that functions mechanically by interacting with other proteins to influence gene transcription. It has a molecular mass of approximately 19 kDa. YAF2 is expressed in a wide range of tissues; notable expression can be found in the nucleus where it can modulate the function of other nuclear factors. It associates particularly with regions of chromatin and is involved in the regulation of transcriptional activity.

Biological function summary

YAF2 plays a role in regulating cell proliferation and apoptosis. It is part of the polycomb group complex which modifies chromatin structure and directly impacts gene expressivity. YAF2 interfaces with proteins like YY1 functioning as a transcription cofactor. It modulates gene expression by either repressing or enhancing transcription depending on the context and specific gene targets being acted upon.

Pathways

This protein is involved in the Wnt signaling pathway and the cell cycle regulation pathway. In the Wnt signaling pathway YAF2 influences the transcriptional regulation of target genes by impacting cellular growth and differentiation processes. It is also related to proteins within these pathways such as β-catenin in the Wnt pathway where it can potentially modulate downstream effects. Through regulating the cell cycle YAF2 impacts the transition between different cell cycle stages interfacing with proteins like cyclins that control progression through the cell cycle.

Alterations in YAF2 activity relate to cancer and developmental disorders. Aberrant expression or mutations in YAF2 have been linked to oncogenic processes particularly in the context of its interactions with YY1 which is a known factor involved in various cancers. Additionally disruptions in its role within the polycomb group complex may contribute to developmental impairments affecting how gene silencing is orchestrated during embryogenesis. Further investigations into YAF2 and its interactions could provide insights into therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to MYC and inhibits MYC-mediated transactivation. Also binds to MYCN and enhances MYCN-dependent transcriptional activation. Increases calpain 2-mediated proteolysis of YY1 in vitro. Component of the E2F6.com-1 complex, a repressive complex that methylates 'Lys-9' of histone H3, suggesting that it is involved in chromatin-remodeling.

See full target information YAF2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:322 PubMed41024119

2025

Transcription factor MYB regulates glycolysis to promote the transfer and malignant progression of gastric cancer through the SSBP2/ISL1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenni Sun,Mi Zhou,Yasai Yao,Xuehong Chen,Tao Qin,Yafei Han,Lu Yue,Ruyong Yao

Molecular medicine reports 23: PubMed33878859

2021

Cryptotanshinone interferes with chondrocyte apoptosis in osteoarthritis by inhibiting the expression of miR‑574‑5p.

Applications

Unspecified application

Species

Unspecified reactive species

Songtao Yue,Xiaochuan Su,Junyan Teng,Jiangyi Wang,Malong Guo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-YAF2 antibody [EPR11020]

Western blot - Anti-YAF2 antibody [EPR11020] (AB177945)

Immunoprecipitation - Anti-YAF2 antibody [EPR11020] (AB177945)

Western blot - Anti-YAF2 antibody [EPR11020] (AB177945)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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Secondary Antibodies

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Product promise