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AB250017

Anti-YAF2 antibody [EPR11020] - BSA and Azide free

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Rabbit Recombinant Monoclonal YAF2 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

YY1-associated factor 2, YAF2

3 Images
Immunoprecipitation - Anti-YAF2 antibody [EPR11020] - BSA and Azide free (AB250017)
  • IP

Supplier Data

Immunoprecipitation - Anti-YAF2 antibody [EPR11020] - BSA and Azide free (AB250017)

This data was developed using ab177945, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from (1) Human skeletal muscle lysate or (2) 1X PBS (negative control) using ab177945 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-YAF2 antibody [EPR11020] (<a href='/en-us/products/primary-antibodies/yaf2-antibody-epr11020-ab177945'>ab177945</a>)

Predicted band size: 20 kDa

false

Western blot - Anti-YAF2 antibody [EPR11020] - BSA and Azide free (AB250017)
  • WB

Supplier Data

Western blot - Anti-YAF2 antibody [EPR11020] - BSA and Azide free (AB250017)

This data was developed using ab177945, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-YAF2 antibody [EPR11020] (<a href='/en-us/products/primary-antibodies/yaf2-antibody-epr11020-ab177945'>ab177945</a>) at 1/1000 dilution

Lane 1:

Human skeletal muscle lysate at 10 µg

Lane 2:

Fetal heart lysate at 10 µg

Predicted band size: 20 kDa

false

Western blot - Anti-YAF2 antibody [EPR11020] - BSA and Azide free (AB250017)
  • WB

Lab

Western blot - Anti-YAF2 antibody [EPR11020] - BSA and Azide free (AB250017)

Blocking and diluting buffer : 5% NFDM/TBST.

Lane 1 : 3 seconds

Lane 2-6 : 180 seconds

Negative sample : human kidney, human pancreas (PMID : 11953439).

This antibody is not suitable to detect YAF2 in cell lines.

All lanes:

Western blot - Anti-YAF2 antibody [EPR11020] (<a href='/en-us/products/primary-antibodies/yaf2-antibody-epr11020-ab177945'>ab177945</a>) at 1/1000 dilution

Lane 1:

Human heart lysate at 20 µg

Lane 2:

Human kidney lysate at 20 µg

Lane 3:

Human pancreas lysate at 20 µg

Lane 4:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

HEK293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 6:

HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 20 kDa

Observed band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11020

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is not suitable to detect YAF2 in cell lines.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab250017 is the carrier-free version of ab177945.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

YAF2 also known as YY1AP1 is a protein that functions mechanically by interacting with other proteins to influence gene transcription. It has a molecular mass of approximately 19 kDa. YAF2 is expressed in a wide range of tissues; notable expression can be found in the nucleus where it can modulate the function of other nuclear factors. It associates particularly with regions of chromatin and is involved in the regulation of transcriptional activity.
Biological function summary

YAF2 plays a role in regulating cell proliferation and apoptosis. It is part of the polycomb group complex which modifies chromatin structure and directly impacts gene expressivity. YAF2 interfaces with proteins like YY1 functioning as a transcription cofactor. It modulates gene expression by either repressing or enhancing transcription depending on the context and specific gene targets being acted upon.

Pathways

This protein is involved in the Wnt signaling pathway and the cell cycle regulation pathway. In the Wnt signaling pathway YAF2 influences the transcriptional regulation of target genes by impacting cellular growth and differentiation processes. It is also related to proteins within these pathways such as β-catenin in the Wnt pathway where it can potentially modulate downstream effects. Through regulating the cell cycle YAF2 impacts the transition between different cell cycle stages interfacing with proteins like cyclins that control progression through the cell cycle.

Alterations in YAF2 activity relate to cancer and developmental disorders. Aberrant expression or mutations in YAF2 have been linked to oncogenic processes particularly in the context of its interactions with YY1 which is a known factor involved in various cancers. Additionally disruptions in its role within the polycomb group complex may contribute to developmental impairments affecting how gene silencing is orchestrated during embryogenesis. Further investigations into YAF2 and its interactions could provide insights into therapeutic targets for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to MYC and inhibits MYC-mediated transactivation. Also binds to MYCN and enhances MYCN-dependent transcriptional activation. Increases calpain 2-mediated proteolysis of YY1 in vitro. Component of the E2F6.com-1 complex, a repressive complex that methylates 'Lys-9' of histone H3, suggesting that it is involved in chromatin-remodeling.
See full target information YAF2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com