Anti-YAP1 antibody [EP1674Y] - BSA and Azide free

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 antibody [EP1674Y] - BSA and Azide free (AB172373)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid carcinoma tissue sections labeling YAP1 with Purified ab52771 at 1 : 50 dilution (1.78 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52771).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-YAP1 antibody [EP1674Y] - BSA and Azide free (AB172373)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling YAP1 with purified ab52771 at 1/20 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52771).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-YAP1 antibody [EP1674Y] - BSA and Azide free (AB172373)

This ICC/IF data was generated using the same anti-YAP1 antibody clone, EP1674Y, in a different buffer formulation (cat# ab52771).

Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling YAP1 with purified ab52771 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised by 0.1% tritonX-100. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (ab150077). Nuclei counterstained with DAPI (blue).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-YAP1 antibody [EP1674Y] - BSA and Azide free (AB172373)

Overlay histogram showing HeLa cells stained with unpurified ab52771 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52771, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52771).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 antibody [EP1674Y] - BSA and Azide free (AB172373)

This IHC data was generated using the same anti-YAP1 antibody clone, EP1674Y, in a different buffer formulation (cat# ab52771).

Immunohistochemical staining of YAP1 in paraffin embedded human breast carcinoma tissue using ab52771 at a 1/100 dilution.

• IP

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Immunoprecipitation - Anti-YAP1 antibody [EP1674Y] - BSA and Azide free (AB172373)

ab52771 (purified) at 1 : 20 dilution (0.5μg) immunoprecipitating YAP1 in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab52771 & HeLa whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab52771 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52771).

All lanes:

Immunoprecipitation - Anti-YAP1 antibody [EP1674Y] (<a href='/en-us/products/primary-antibodies/yap1-antibody-ep1674y-ab52771'>ab52771</a>)

Predicted band size: 54 kDa

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