Anti-YAP1 (phospho S127) antibody [EP1675Y]
- BOND RX™ Validated
- 20ul selling size
- RabMAb
- Recombinant
- What is this?
4
(3 Reviews)
|
(151 Publications)
Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab76252) is a rabbit monoclonal antibody detecting YAP1 in Western Blot, IHC-P, Dot Blot. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 100 publications
- Trusted since 2009
View Alternative Names
YAP65, YAP1, Transcriptional coactivator YAP1, Yes-associated protein 1, Protein yorkie homolog, Yes-associated protein YAP65 homolog
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 (phospho S127) antibody [EP1675Y] (AB76252)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human adenocarcinoma of endometrium tissue labelling YAP1 (phospho S127) with purified ab76252 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 (phospho S127) antibody [EP1675Y] (AB76252)
Immunohistochemical analysis of paraffin-embedded sections of human endometrium cancer tissue labelling YAP1 (S127) with ab76252 at 1/500 (0.428 μg/ml) dilution, followed by ready to use secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counter stained with Hematoxylin.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Positive staining on human endometrium cancer without alkaline phosphatase treatment; No signal was detected when tissues were treated with alkaline phosphatase (image B).
The section was incubated with ab76252 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 (phospho S127) antibody [EP1675Y] (AB76252)
Immunohistochemical analysis of paraffin-embedded sections of rat kidney tissue labelling YAP1 (S127) with ab76252 at 1/500 (0.428 μg/ml) dilution, followed by ready to use secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counter stained with Hematoxylin.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Positive staining on rat kidney without alkaline phosphatase treatment; No signal was detected when tissues were treated with alkaline phosphatase (image B).
The section was incubated with ab76252 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 (phospho S127) antibody [EP1675Y] (AB76252)
Immunohistochemical analysis of paraffin-embedded sections of mouse stomach tissue labelling YAP1 (S127) with ab76252 at 1/500 (0.428 μg/ml) dilution, followed by ready to use secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counter stained with Hematoxylin.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Positive staining on mouse stomach without alkaline phosphatase treatment; No signal was detected when tissues were treated with alkaline phosphatase (image B).
The section was incubated with ab76252 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 (phospho S127) antibody [EP1675Y] (AB76252)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue labelling YAP1 (phospho S127) with purified ab76252 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 (phospho S127) antibody [EP1675Y] (AB76252)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling YAP1 (phospho S127) with purified ab76252 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
- WB
Supplier Data
Western blot - Anti-YAP1 (phospho S127) antibody [EP1675Y] (AB76252)
Blocking buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab76252) at 1/1000 dilution
Lane 1:
Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2:
HeLa treated with 100ng/ml Calyculin A for 30 min whole cell lysate at 15 µg
Lane 3:
HeLa treated with 100ng/ml Calyculin A for 30 min whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 75 kDa
true
Exposure time: 180s
- WB
Lab
Western blot - Anti-YAP1 (phospho S127) antibody [EP1675Y] (AB76252)
Blocking and dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab76252) at 1/1000 dilution
Lane 1:
293A grown in serum-free media overnight, whole cell lysate at 20 µg
Lane 2:
293A grown in serum-free media overnight, then 10% FBS was added to medium for 1 hour, whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 75 kDa
false
Exposure time: 3min
- WB
Supplier Data
Western blot - Anti-YAP1 (phospho S127) antibody [EP1675Y] (AB76252)
Blocking buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab76252) at 1/1000 dilution
Lane 1:
Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg
Lane 2:
NIH/3T3 treated with 100ng/ml Calyculin A for 30 min whole cell lysate at 15 µg
Lane 3:
NIH/3T3 treated with 100ng/ml Calyculin A for 30 min whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 75 kDa
true
Exposure time: 40s
- WB
Supplier Data
Western blot - Anti-YAP1 (phospho S127) antibody [EP1675Y] (AB76252)
Blocking buffer : 5% NFDM/TBST
All lanes:
Western blot - Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab76252) at 1/1000 dilution
Lane 1:
Untreated C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg
Lane 2:
C6 treated with 100ng/ml Calyculin A for 30 min whole cell lysate at 15 µg
Lane 3:
C6 treated with 100ng/ml Calyculin A for 30 min whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 75 kDa
true
Exposure time: 60s
- Dot
Supplier Data
Dot Blot - Anti-YAP1 (phospho S127) antibody [EP1675Y] (AB76252)
Dot blot analysis of YAP1 peptides labelling YAP1 (pS127) with ab76252 at 1/1000 dilution (0.24μg/ml). ab97051 (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated) was used as the secondary antibody at 1/100000 dilution.
Lane 1 : YAP1 non-phospho peptide
Lane 2 : YAP1 S127 phospho peptide
Lane 3 : YAP1 S128 phospho peptide
Lane 4 : YAP1 S131 phospho peptide
Lane 5 : YAP1 S127+S128 phospho peptide
Lane 6 : YAP1 S127+S131 phospho peptide
Lane 7 : YAP1 S128+S131 phospho peptide
Lane 8 : YAP1 S127+S128+S131 phospho peptide
Blocking and dilution buffer : 5% NFDM/TBST.
Exposure time : 3 minutes.
Related conjugates and formulations (4)
-
Anti-YAP1 (phospho S127) antibody [EP1675Y] - BSA and Azide free
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-YAP1 (phospho S127) antibody [EP1675Y]
-
603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-YAP1 (phospho S127) antibody [EP1675Y]
-
565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-YAP1 (phospho S127) antibody [EP1675Y]
Reactivity data
Product details
What is this antibody validated in?
Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab76252) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Dot Blot in Human, Mouse, Rat samples.
What is the molecular weight of YAP1?
Anti-YAP1 (phospho S127) [EP1675Y] (ab76252) specifically detects a band for YAP1 (UniProt: P46937) at a molecular weight of 65 - 75kDa.
Trusted by the scientific community
Anti-YAP1 (phospho S127) [EP1675Y] (ab76252) was first used in a scientific publication in 2009 and has been cited over 100 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EP1675Y] also available for your convenience: ab76252, Carrier free - ab172374, Alexa Fluor® 488 - ab310956, Alexa Fluor® 647 - ab311073, Alexa Fluor® 568 - ab312934, Alexa Fluor® 555 - ab313146
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
YAP1 influences cell proliferation and apoptosis by interacting with other proteins to form transcriptional complexes. It participates in promoting organ size and tissue regeneration. YAP1's activity is regulated through phosphorylation primarily at serine 127 which determines its subcellular localization and transcriptional activity. The phosphorylated form often referred to as phospho-YAP remains in the cytoplasm while dephosphorylated YAP1 translocates to the nucleus where it contributes to transcriptional activation.
Pathways
YAP1 is importantly involved in the Hippo signaling pathway and Wnt/Β-catenin signaling pathway. In the Hippo pathway kinases LATS1/2 phosphorylate YAP1 preventing its nuclear localization and promoting cell apoptosis when required. In contrast in the Wnt/Β-catenin pathway YAP1 interacts with Β-catenin to regulate gene expression related to proliferation. Proteins such as TAZ which shares structural and functional similarities with YAP1 often collaborate in these pathways influencing cellular growth and differentiation.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (151)
Recent publications for all applications. Explore the full list and refine your search
Journal of ovarian research 18:170 PubMed40739575
2025
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Journal of nanobiotechnology 23:506 PubMed40660260
2025
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Frontiers in oncology 15:1586679 PubMed40626009
2025
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European journal of histochemistry : EJH 69: PubMed40421484
2025
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Cell death & disease 16:380 PubMed40368918
2025
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Science advances 11:eadt4881 PubMed40249803
2025
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Cell death & disease 16:278 PubMed40216763
2025
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Cell division 20:7 PubMed40069841
2025
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Translational oncology 54:102346 PubMed40056528
2025
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Nature communications 16:749 PubMed39820504
2025
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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