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Rabbit Recombinant Multiclonal YAP1 phospho S127 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human YAP1 phospho S127.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-YAP1 (phospho S127) Antibody [RP23040163] (AB313464), expandable thumbnail
  • Western blot - Anti-YAP1 (phospho S127) Antibody [RP23040163] (AB313464), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form
Liquid
Clonality
Multiclonal

Immunogen

  • Synthetic Peptide within Human YAP1 phospho S127. Database link P46937

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
2 µg/mL
Notes

-

Target data

Function

Transcriptional regulator with dual roles as a coactivator and corepressor. Critical downstream regulatory target in the Hippo signaling pathway, crucial for organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637, PubMed:30447097). The Hippo signaling pathway core involves a kinase cascade featuring STK3/MST2 and STK4/MST1, along with its regulatory partner SAV1, which phosphorylates and activates LATS1/2 in complex with their regulatory protein, MOB1. This activation leads to the phosphorylation and inactivation of the YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288). Phosphorylation of YAP1 by LATS1/2 prevents its nuclear translocation, thereby regulating the expression of its target genes (PubMed:18158288, PubMed:26598551, PubMed:34404733). The transcriptional regulation of gene expression requires TEAD transcription factors and modulates cell growth, anchorage-independent growth, and induction of epithelial-mesenchymal transition (EMT) (PubMed:18579750). Plays a key role in tissue tension and 3D tissue shape by regulating the cortical actomyosin network, acting via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702). It also suppresses ciliogenesis by acting as a transcriptional corepressor of TEAD4 target genes AURKA and PLK1 (PubMed:25849865). In conjunction with WWTR1, regulates TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (By similarity). Synergizes with WBP2 to enhance PGR activity (PubMed:16772533). Isoform 2. Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3). Isoform 3. Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3).

Alternative names

YAP65, YAP1, Transcriptional coactivator YAP1, Yes-associated protein 1, Protein yorkie homolog, Yes-associated protein YAP65 homolog

Recommended products

Rabbit Recombinant Multiclonal YAP1 phospho S127 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human YAP1 phospho S127.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogen
  • Synthetic Peptide within Human YAP1 phospho S127. Database link P46937
Clone number
RP23040163
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.

Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

YAP1 also known as Yes-associated protein 1 is a transcriptional coactivator involved in regulating gene expression. This protein has a mass of approximately 65 kDa and is expressed in various tissues including the liver and heart. YAP1 plays a significant role in the Hippo signaling pathway where it acts by binding to transcriptional enhancer factors such as TEADs to activate gene transcription.

Biological function summary

YAP1 influences cell proliferation and apoptosis by interacting with other proteins to form transcriptional complexes. It participates in promoting organ size and tissue regeneration. YAP1's activity is regulated through phosphorylation primarily at serine 127 which determines its subcellular localization and transcriptional activity. The phosphorylated form often referred to as phospho-YAP remains in the cytoplasm while dephosphorylated YAP1 translocates to the nucleus where it contributes to transcriptional activation.

Pathways

YAP1 is importantly involved in the Hippo signaling pathway and Wnt/β-catenin signaling pathway. In the Hippo pathway kinases LATS1/2 phosphorylate YAP1 preventing its nuclear localization and promoting cell apoptosis when required. In contrast in the Wnt/β-catenin pathway YAP1 interacts with β-catenin to regulate gene expression related to proliferation. Proteins such as TAZ which shares structural and functional similarities with YAP1 often collaborate in these pathways influencing cellular growth and differentiation.

Associated diseases and disorders

YAP1's dysregulation links to cancer development notably in liver and breast cancer. Overexpression or nuclear accumulation of YAP1 leads to uncontrollable cell growth and tumorigenesis. Its interaction with the TEAD transcription factors highlights its significance in oncogenesis. Furthermore YAP1 also connects with pathways involving other proteins like TGF-β contributing to fibrosis-related disorders such as pulmonary fibrosis where unexpected remodeling and excessive tissue growth occur.

Product promise

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2 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-YAP1 (phospho S127) Antibody [RP23040163] (ab313464), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-YAP1 (phospho S127) Antibody [RP23040163] (ab313464)

    For immunofluorescence analysis, PC-3 cells were fixed and permeabilized for detection of endogenous YAP pS (127) using ab313464 (2 ug/ml) and labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate ( 1:2000). Panel a) shows representative cells that were stained for detection and localization of YAP pS (127) protein (green), Panel b) is stained for nuclei (blue) using DAPI . Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin ( 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of YAP pS (127). Panel e) shows loss of signal by competition with the YAP pS (127) peptide, demonstrating antibody specificity, and panel f) demonstrates no competition with the non-phospho peptide. Panel g) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

  • Western blot - Anti-YAP1 (phospho S127) Antibody [RP23040163] (ab313464), expandable thumbnail

    Western blot - Anti-YAP1 (phospho S127) Antibody [RP23040163] (ab313464)

    A 54 kDa band corresponding to YAP (pS127) was observed across the cell lines tested. Known quantity of protein samples were electrophoresed 4-12% Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk.

    All lanes: Western blot - Anti-YAP1 (phospho S127) Antibody [RP23040163] (ab313464) at 2 µg/mL

    Lane 1: Membrane extracts of U-87 MG at 30 µg

    Lane 2: Membrane extracts of HeLa at 30 µg

    Lane 3: Membrane extracts of NTERA-2 at 30 µg

    Lane 4: Membrane extracts of PC-3 at 30 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

    Developed using the ECL technique.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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