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BiochemicalsProteins and Peptides
Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
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Rabbit Polyclonal antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 51 publications. Immunogen corresponding to Synthetic Peptide within Human YAP1 phospho Y357 aa 350-400 conjugated to Keyhole Limpet Haemocyanin.
IgG
Rabbit
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS
Liquid
Polyclonal
WB | ICC/IF | |
---|---|---|
Human | Tested | Expected |
Mouse | Predicted | Predicted |
Rat | Predicted | Predicted |
Chicken | Predicted | Predicted |
Chimpanzee | Predicted | Predicted |
Cow | Predicted | Predicted |
Dog | Predicted | Predicted |
Horse | Predicted | Predicted |
Opossum | Predicted | Predicted |
Xenopus laevis | Predicted | Predicted |
Zebrafish | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Horse, Chicken, Cow, Dog, Xenopus laevis, Zebrafish, Opossum, Chimpanzee | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Horse, Chicken, Cow, Dog, Xenopus laevis, Zebrafish, Opossum, Chimpanzee | Dilution info - | Notes - |
Rabbit Polyclonal antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 51 publications. Immunogen corresponding to Synthetic Peptide within Human YAP1 phospho Y357 aa 350-400 conjugated to Keyhole Limpet Haemocyanin.
IgG
Rabbit
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS
Liquid
Polyclonal
Affinity purification Immunogen
ab62751 was affinity-purified using the immunizing peptide immobilized on agarose.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
YAP1 influences cell proliferation and apoptosis by interacting with other proteins to form transcriptional complexes. It participates in promoting organ size and tissue regeneration. YAP1's activity is regulated through phosphorylation primarily at serine 127 which determines its subcellular localization and transcriptional activity. The phosphorylated form often referred to as phospho-YAP remains in the cytoplasm while dephosphorylated YAP1 translocates to the nucleus where it contributes to transcriptional activation.
YAP1 also known as Yes-associated protein 1 is a transcriptional coactivator involved in regulating gene expression. This protein has a mass of approximately 65 kDa and is expressed in various tissues including the liver and heart. YAP1 plays a significant role in the Hippo signaling pathway where it acts by binding to transcriptional enhancer factors such as TEADs to activate gene transcription.
YAP1 is importantly involved in the Hippo signaling pathway and Wnt/β-catenin signaling pathway. In the Hippo pathway kinases LATS1/2 phosphorylate YAP1 preventing its nuclear localization and promoting cell apoptosis when required. In contrast in the Wnt/β-catenin pathway YAP1 interacts with β-catenin to regulate gene expression related to proliferation. Proteins such as TAZ which shares structural and functional similarities with YAP1 often collaborate in these pathways influencing cellular growth and differentiation.
YAP1's dysregulation links to cancer development notably in liver and breast cancer. Overexpression or nuclear accumulation of YAP1 leads to uncontrollable cell growth and tumorigenesis. Its interaction with the TEAD transcription factors highlights its significance in oncogenesis. Furthermore YAP1 also connects with pathways involving other proteins like TGF-β contributing to fibrosis-related disorders such as pulmonary fibrosis where unexpected remodeling and excessive tissue growth occur.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
All lanes: Western blot - Anti-YAP1 (phospho Y357) antibody (AB62751) at 1/2000 dilution
Lanes 1, 3 and 4: Whole cell lysates of HEK-293T cells co-transfected with human YAP1 and human c-Abl
Lane 2: Whole cell lysates of untransfected HEK-293T cells
All lanes: Goat Anti-Rabbit IgG, Peroxidase conjugate
Predicted band size: 54 kDa
Observed band size: 100 kDa, 65 kDa, 70 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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