Anti-YAP1 (phospho Y357) antibody (ab62751) is a rabbit polyclonal antibody that is used to detect YAP1 in Western Blot, ICC/IF. Suitable for Human samples.
- Over 50 publications
- Trusted since 2008
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- Advanced healthcare materials 12:e23013162023An H S-BMP6 Dual-Loading System with Regulating Yap/Taz and Jun Pathway for Synergistic Critical Limb Ischemia Salvaging Therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiateng Hu,Zhijue Xu,Donghui Liao,Yihong Jiang,Hongji Pu,Zhaoyu Wu,Xintong Xu,Zhen Zhao,Jianqiang Liu,Xinwu Lu,Xiaobing Liu,Bo LiPubMed 37531238
- British journal of pharmacology 180:1168-11852022Curaxin CBL0137 inhibits endothelial inflammation and atherogenesis via suppression of the Src-YAP signalling axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuanyu Ding,Minchun Jiang,Chi Wai Lau,Jianfang Luo,Andrew M Chan,Li Wang,Yu HuangPubMed 36495259
- Frontiers in pharmacology 13:10572762022The essential role of YAP in ERα36-mediated proliferation and the epithelial-mesenchymal transition in MCF-7 breast cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiso Park,Seung Hyun Lee,Quyen Thu Bui,Young-Mi Kim,Keon Wook KangPubMed 36534032
- Molecular cancer therapeutics 21:1652-16622022Opposite Effects of Src Family Kinases on YAP and ERK Activation in Pancreatic Cancer Cells: Implications for Targeted Therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJames Sinnett-Smith,Tarique Anwar,Elaine F Reed,Yaroslav Teper,Guido Eibl,Enrique RozengurtPubMed 35999654
- Neuro-oncology 24:2107-21202022FRK inhibits glioblastoma progression via phosphorylating YAP and inducing its ubiquitylation and degradation by Siah1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Wang,Kai Wang,Jiale Fu,Yu Zhang,Yufei Mao,Xu Wang,Xiang Wang,Rutong Yu,Xiuping ZhouPubMed 35723276
- JCI insight 7:2022Senescent hepatic stellate cells promote liver regeneration through IL-6 and ligands of CXCR2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaiyuan Cheng,Ki-Hyun Kim,Lester F LauPubMed 35708907
- Open medicine (Warsaw, Poland) 17:453-4622022LMO3 promotes proliferation and metastasis of papillary thyroid carcinoma cells by regulating LIMK1-mediated cofilin and the β-catenin pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZeyi Ling,Xiaoli Long,Ying Wu,Jie Li,Mingliang FengPubMed 35350839
- Frontiers in oncology 12:8396032022α-Hederin Inhibits the Proliferation of Hepatocellular Carcinoma Cells Hippo-Yes-Associated Protein Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTongqing Chen,Dongdong Sun,Qijuan Wang,Tingting Zhou,Jiani Tan,Changliang Xu,Haibo Cheng,Weixing ShenPubMed 35311132
- Science signaling 15:eabj47432022Signaling by the tyrosine kinase Yes promotes liver cancer development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJean-Philippe Guégan,Marjorie Lapouge,Laure Voisin,Marc K Saba-El-Leil,Pierre-Luc Tanguay,Kim Lévesque,Jérémy Brégeon,Anne-Marie Mes-Masson,Daniel Lamarre,Benjamin Haibe-Kains,Vincent Q Trinh,Geneviève Soucy,Marc Bilodeau,Sylvain MelochePubMed 35041461
- Investigative ophthalmology & visual science 63:162022Matrix Mechanotransduction via Yes-Associated Protein in Human Lamina Cribrosa Cells in Glaucoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRory Murphy,Mustapha Irnaten,Alan Hopkins,Jeffrey O'Callaghan,W Daniel Stamer,Abbot F Clark,Deborah Wallace,Colm J O'BrienPubMed 35015027
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human YAP1 phospho Y357 aa 350-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P46937
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Opossum | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Horse, Chicken, Cow, Dog, Xenopus laevis, Zebrafish, Opossum, Chimpanzee | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Horse, Chicken, Cow, Dog, Xenopus laevis, Zebrafish, Opossum, Chimpanzee | Dilution info - | Notes - |
Associated Products
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Anti-YAP1 (phospho Y357) antibody (ab62751) is a rabbit polyclonal antibody that is used to detect YAP1 in Western Blot, ICC/IF. Suitable for Human samples.
- Over 50 publications
- Trusted since 2008
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human YAP1 phospho Y357 aa 350-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P46937
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab62751 was affinity-purified using the immunizing peptide immobilized on agarose.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Anti-YAP1 (phospho Y357) antibody (ab62751) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of YAP1?
Anti-YAP1 (phospho Y357) (ab62751) specifically detects a band for YAP1 (UniProt: P46937) at a molecular weight of 65kDa.
Trusted by the scientific community
Anti-YAP1 (phospho Y357) (ab62751) was first used in a scientific publication in 2008 and has been cited over 50 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
YAP1 also known as Yes-associated protein 1 is a transcriptional coactivator involved in regulating gene expression. This protein has a mass of approximately 65 kDa and is expressed in various tissues including the liver and heart. YAP1 plays a significant role in the Hippo signaling pathway where it acts by binding to transcriptional enhancer factors such as TEADs to activate gene transcription.
- Biological function summary
YAP1 influences cell proliferation and apoptosis by interacting with other proteins to form transcriptional complexes. It participates in promoting organ size and tissue regeneration. YAP1's activity is regulated through phosphorylation primarily at serine 127 which determines its subcellular localization and transcriptional activity. The phosphorylated form often referred to as phospho-YAP remains in the cytoplasm while dephosphorylated YAP1 translocates to the nucleus where it contributes to transcriptional activation.
- Pathways
YAP1 is importantly involved in the Hippo signaling pathway and Wnt/Β-catenin signaling pathway. In the Hippo pathway kinases LATS1/2 phosphorylate YAP1 preventing its nuclear localization and promoting cell apoptosis when required. In contrast in the Wnt/Β-catenin pathway YAP1 interacts with Β-catenin to regulate gene expression related to proliferation. Proteins such as TAZ which shares structural and functional similarities with YAP1 often collaborate in these pathways influencing cellular growth and differentiation.
- Associated diseases and disorders
YAP1's dysregulation links to cancer development notably in liver and breast cancer. Overexpression or nuclear accumulation of YAP1 leads to uncontrollable cell growth and tumorigenesis. Its interaction with the TEAD transcription factors highlights its significance in oncogenesis. Furthermore YAP1 also connects with pathways involving other proteins like TGF-Β contributing to fibrosis-related disorders such as pulmonary fibrosis where unexpected remodeling and excessive tissue growth occur.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Western blot - Anti-YAP1 (phospho Y357) antibody (ab62751)
YAP1 (phospho Y357) Western blot staining using rabbit Anti-YAP1 (phospho Y357) antibody
All lanes: Western blot - Anti-YAP1 (phospho Y357) antibody (ab62751) at 1/2000 dilution
Lane 1: Whole cell lysates of HEK-293T cells co-transfected with human YAP1 and human c-Abl
Lane 2: Whole cell lysates of untransfected HEK-293T cells
Lane 3: Whole cell lysates of HEK-293T cells co-transfected with human YAP1 and human c-Abl with YAP1 peptide (human 351-362)
Lane 4: Whole cell lysates of HEK-293T cells co-transfected with human YAP1 and human c-Abl with phospho-YAP1 (human 351-362 [phospho Y357]) immunizing peptide
SecondaryAll lanes: Goat Anti-Rabbit IgG, Peroxidase conjugate
Predicted band size: 54 kDa
Observed band size: 100 kDa, 65 kDa, 70 kDa
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