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Rabbit Polyclonal antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 51 publications. Immunogen corresponding to Synthetic Peptide within Human YAP1 phospho Y357 aa 350-400 conjugated to Keyhole Limpet Haemocyanin.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human YAP1 phospho Y357 aa 350-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P46937
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Expected
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Chicken
Predicted
Predicted
Chimpanzee
Predicted
Predicted
Cow
Predicted
Predicted
Dog
Predicted
Predicted
Horse
Predicted
Predicted
Opossum
Predicted
Predicted
Xenopus laevis
Predicted
Predicted
Zebrafish
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

0.5-1 µg/mL

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Horse, Chicken, Cow, Dog, Xenopus laevis, Zebrafish, Opossum, Chimpanzee

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Horse, Chicken, Cow, Dog, Xenopus laevis, Zebrafish, Opossum, Chimpanzee

Dilution info

-

Notes

-

Recommended products

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Rabbit Polyclonal antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 51 publications. Immunogen corresponding to Synthetic Peptide within Human YAP1 phospho Y357 aa 350-400 conjugated to Keyhole Limpet Haemocyanin.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human YAP1 phospho Y357 aa 350-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P46937
Purification technique

Affinity purification Immunogen

Concentration
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Purification notes

ab62751 was affinity-purified using the immunizing peptide immobilized on agarose.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

YAP1 influences cell proliferation and apoptosis by interacting with other proteins to form transcriptional complexes. It participates in promoting organ size and tissue regeneration. YAP1's activity is regulated through phosphorylation primarily at serine 127 which determines its subcellular localization and transcriptional activity. The phosphorylated form often referred to as phospho-YAP remains in the cytoplasm while dephosphorylated YAP1 translocates to the nucleus where it contributes to transcriptional activation.

Activity summary

YAP1 also known as Yes-associated protein 1 is a transcriptional coactivator involved in regulating gene expression. This protein has a mass of approximately 65 kDa and is expressed in various tissues including the liver and heart. YAP1 plays a significant role in the Hippo signaling pathway where it acts by binding to transcriptional enhancer factors such as TEADs to activate gene transcription.

Pathways

YAP1 is importantly involved in the Hippo signaling pathway and Wnt/β-catenin signaling pathway. In the Hippo pathway kinases LATS1/2 phosphorylate YAP1 preventing its nuclear localization and promoting cell apoptosis when required. In contrast in the Wnt/β-catenin pathway YAP1 interacts with β-catenin to regulate gene expression related to proliferation. Proteins such as TAZ which shares structural and functional similarities with YAP1 often collaborate in these pathways influencing cellular growth and differentiation.

Associated diseases and disorders

YAP1's dysregulation links to cancer development notably in liver and breast cancer. Overexpression or nuclear accumulation of YAP1 leads to uncontrollable cell growth and tumorigenesis. Its interaction with the TEAD transcription factors highlights its significance in oncogenesis. Furthermore YAP1 also connects with pathways involving other proteins like TGF-β contributing to fibrosis-related disorders such as pulmonary fibrosis where unexpected remodeling and excessive tissue growth occur.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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1 product image

  • Western blot - Anti-YAP1 (phospho Y357) antibody (ab62751), expandable thumbnail

    Western blot - Anti-YAP1 (phospho Y357) antibody (ab62751)

    All lanes: Western blot - Anti-YAP1 (phospho Y357) antibody (AB62751) at 1/2000 dilution

    Lanes 1, 3 and 4: Whole cell lysates of HEK-293T cells co-transfected with human YAP1 and human c-Abl

    Lane 2: Whole cell lysates of untransfected HEK-293T cells

    Secondary

    All lanes: Goat Anti-Rabbit IgG, Peroxidase conjugate

    Predicted band size: 54 kDa

    Observed band size: 100 kDa, 65 kDa, 70 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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