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AB228957

Anti-YARS2/TyRS antibody

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(2 Publications)

Rabbit Polyclonal YARS2/TyRS antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human YARS2.

View Alternative Names

CGI-04, YARS2, Tyrosyl-tRNA synthetase, TyrRS

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YARS2/TyRS antibody (AB228957)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YARS2/TyRS antibody (AB228957)

Paraffin-embedded human colon carcinoma tissue stained for YARS2/TyrRS using ab228957 at 1/250 dilution in immunohistochemical analysis.

Western blot - Anti-YARS2/TyRS antibody (AB228957)
  • WB

Supplier Data

Western blot - Anti-YARS2/TyRS antibody (AB228957)

10% SDS PAGE gel.

All lanes:

Western blot - Anti-YARS2/TyRS antibody (ab228957) at 1/1000 dilution

All lanes:

NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human YARS2. The exact immunogen used to generate this antibody is proprietary information.

Q9Y2Z4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

YARS2 also known as TyrRS is a mitochondrial tyrosyl-tRNA synthetase with a molecular mass of approximately 55 kDa. It catalyzes the attachment of tyrosine to its corresponding tRNA a fundamental step for protein synthesis within mitochondria. YARS2 is expressed in various tissues with notable levels in those requiring high mitochondrial activity. It ensures the correct translation of mitochondrial genes by ensuring the specific aminoacylation of tRNA.
Biological function summary

YARS2 plays an essential role in mitochondrial protein biosynthesis a process vital for cellular energy production. As part of the group of aminoacyl-tRNA synthetases it is required for proper mitochondrial function and cellular respiration. Mitochondrial dysfunction due to errors in this enzyme can disrupt cellular homeostasis and energy metabolism leading to severe outcomes.

Pathways

The activity of YARS2 is integral to the oxidative phosphorylation pathway. This pathway involves the production of ATP the energy currency of the cell and requires a coordinated action of multiple proteins including cytochrome c oxidase subunits. YARS2 ensures the synthesis of key proteins necessary for this pathway influencing both energy generation and overall cellular health.

YARS2 mutations relate to mitochondrial myopathy and sideroblastic anemia. These conditions often involve the disruption of normal mitochondrial protein synthesis leading to muscle weakness and compromised red blood cell production. Mutations in related proteins such as MTO1 and TRMU which also influence mitochondrial translation can exacerbate these disorders highlighting the critical role of YARS2 within mitochondrial genetic translation and its clinical significance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction : tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
See full target information YARS2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer biology & therapy 23:1-8 PubMed36154909

2022

Targeting mitochondrial tyrosyl-tRNA synthetase YARS2 suppresses colorectal cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Qingxia Fang,Jingyang Lin,Liang Gao,Ruolang Pan,Xiaochun Zheng

Nucleic acids research 47:5341-5355 PubMed30916346

2019

Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Danni Chen,Zengming Zhang,Chao Chen,Shihao Yao,Qingxian Yang,Feng Li,Xiao He,Cheng Ai,Meng Wang,Min-Xin Guan
View all publications
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Product promise

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