Mouse Recombinant Monoclonal Yes1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-Yes antibody [1/Yes] (AB305327), expandable thumbnail](https://content.abcam.com/products/images/yes-antibody-1-yes-ab305327--western-blot-img404522.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Yes antibody [1/Yes] (AB305327)")
![Western blot - Anti-Yes antibody [1/Yes] (AB305327), expandable thumbnail](https://content.abcam.com/products/images/yes-antibody-1-yes-ab305327--western-blot-img404521.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Yes antibody [1/Yes] (AB305327)")
![Western blot - Anti-Yes antibody [1/Yes] (AB305327), expandable thumbnail](https://content.abcam.com/products/images/yes-antibody-1-yes-ab305327--western-blot-img404523.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Yes antibody [1/Yes] (AB305327)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | IHC-P | |
|---|---|---|---|
| Human | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Tested | Not recommended |
| Rat | Not recommended | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGFR, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis. Catalyzes phosphorylation of organic cation transporter OCT2 which induces its transport activity (PubMed:26979622).
Alternative names
YES, YES1, Tyrosine-protein kinase Yes, Proto-oncogene c-Yes, p61-Yes
Recommended products
Mouse Recombinant Monoclonal Yes1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 1-Yes
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Yes1 protein also known as p61-Yes is a member of the Src family kinases (SFKs) and has a molecular mass of approximately 61 kDa. This non-receptor tyrosine kinase engages in phosphorylation of tyrosine residues playing a significant role in cell signaling. It is widely expressed in many tissues including the brain heart and lungs suggesting its involvement in varied physiological processes.
- Biological function summary
The Yes1 protein participates in cellular processes such as cell growth survival and differentiation. It contributes to signaling cascades that regulate these functions often working as part of protein complexes. Its ability to interact with various signaling molecules positions it as an important player in the control of cell behavior particularly in response to extracellular stimuli.
- Pathways
The Yes1 protein integrates into key signaling routes like the epidermal growth factor receptor (EGFR) signaling pathway and the integrin signaling pathway. Within these pathways Yes1 interacts with proteins like c-Src and Fyn which are other members of the Src family. These pathways are vital in mediating cellular communication for growth and attachment highlighting Yes1's role in transmitting signals that prompt cellular responses.
- Associated diseases and disorders
Research connects Yes1 protein to several types of cancer such as colon cancer and breast cancer. The dysregulation of Yes1 along with associated proteins like c-Myc may lead to uncontrolled cell proliferation typical of tumorigenesis. Understanding these interactions offers insights into potential therapeutic targets as altering Yes1 activity might influence cancer progression or treatment outcomes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Yes antibody [1/Yes] (ab305327), expandable thumbnail](https://content.abcam.com/products/images/yes-antibody-1-yes-ab305327--western-blot-img404522.jpg "Western blot - Anti-Yes antibody [1/Yes] (ab305327)")
Western blot - Anti-Yes antibody [1/Yes] (ab305327)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 50 seconds.All lanes: Western blot - Anti-Yes antibody [1/Yes] (ab305327) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 10 µg
Lane 2: HeLa transfected with siRNA specifically targeting Yes1 whole cell lysate at 10 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution
Observed band size: 62 kDa
Exposure time: 50s
![Western blot - Anti-Yes antibody [1/Yes] (ab305327), expandable thumbnail](https://content.abcam.com/products/images/yes-antibody-1-yes-ab305327--western-blot-img404521.jpg "Western blot - Anti-Yes antibody [1/Yes] (ab305327)")
Western blot - Anti-Yes antibody [1/Yes] (ab305327)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 158 seconds.All lanes: Western blot - Anti-Yes antibody [1/Yes] (ab305327) at 1/1000 dilution
All lanes: Western blot at 10 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution
Observed band size: 62 kDa
Exposure time: 158s
![Western blot - Anti-Yes antibody [1/Yes] (ab305327), expandable thumbnail](https://content.abcam.com/products/images/yes-antibody-1-yes-ab305327--western-blot-img404523.jpg "Western blot - Anti-Yes antibody [1/Yes] (ab305327)")
Western blot - Anti-Yes antibody [1/Yes] (ab305327)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
This blot of Lane 4 was developed using a high sensitivity ECL substrate.
Exposure times:
Lane 1-3, 180 seconds
Lane 4, 92 secondsAll lanes: Western blot - Anti-Yes antibody [1/Yes] (ab305327) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg
Lane 2: 293T (human embryonic kidney epithelial cell), whole cell lysate at 10 µg
Lane 3: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg
Lane 4: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution
Developed using the ECL technique.
Observed band size: 62 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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