Anti-Yes antibody [1/Yes] - BSA and Azide Free

• WB

Supplier Data

Western blot - Anti-Yes antibody [1/Yes] - BSA and Azide Free (AB305328)

This data was developed using ab305327, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation. This blot of Lane 4 was developed using a high sensitivity ECL substrate. Exposure times : Lane 1-3, 180 seconds Lane 4, 92 seconds

All lanes:

Western blot - Anti-Yes antibody [1/Yes] (<a href='/en-us/products/primary-antibodies/yes-antibody-1-yes-ab305327'>ab305327</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

293T (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution

Observed band size: 62 kDa

true

• WB

Supplier Data

Western blot - Anti-Yes antibody [1/Yes] - BSA and Azide Free (AB305328)

This data was developed using ab305327, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Exposure time : 158 seconds.

All lanes:

Western blot - Anti-Yes antibody [1/Yes] (<a href='/en-us/products/primary-antibodies/yes-antibody-1-yes-ab305327'>ab305327</a>) at 1/1000 dilution

All lanes:

Western blot at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution

Observed band size: 62 kDa

false

Exposure time: 158s

• WB

Supplier Data

Western blot - Anti-Yes antibody [1/Yes] - BSA and Azide Free (AB305328)

This data was developed using ab305327, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Exposure time : 50 seconds.

All lanes:

Western blot - Anti-Yes antibody [1/Yes] (<a href='/en-us/products/primary-antibodies/yes-antibody-1-yes-ab305327'>ab305327</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 10 µg

Lane 2:

HeLa transfected with siRNA specifically targeting Yes1 whole cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution

Observed band size: 62 kDa

false

Exposure time: 50s