Anti-Yes1 antibody [EPR3172] - BSA and Azide free

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Yes1 antibody [EPR3172] - BSA and Azide free (AB248467)

This data was developed using ab133314, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of Yes1 staining in a section of frozen human normal lung performed on a Leica Biosystems BOND^® RX instrument. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab133314, 1/1000 dilution (1µg/ml), for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Yes1 antibody [EPR3172] - BSA and Azide free (AB248467)

This data was developed using ab133314, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of Yes1 staining in a section of frozen human placenta performed on a Leica Biosystems BOND^® RX instrument. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab133314, 1/1000 dilution (1µg/ml), for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Yes1 antibody [EPR3172] - BSA and Azide free (AB248467)

This data was developed using ab133314, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of Yes1 staining in a section of frozen human frozen breast cancer performed on a Leica Biosystems BOND^® RX instrument. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab133314, 1/1000 dilution (1µg/ml), for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• WB

Unknown

Western blot - Anti-Yes1 antibody [EPR3172] - BSA and Azide free (AB248467)

This data was developed using ab133314, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Yes1 antibody [EPR3172] (<a href='/en-us/products/primary-antibodies/yes1-antibody-epr3172-ab133314'>ab133314</a>) at 1/1000 dilution

Lane 1:

U87-MG cell lysate at 10 µg

Lane 2:

Molt-4 cell lysate at 10 µg

Lane 3:

LnCaP cell lysate at 10 µg

Lane 4:

A431 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 60 kDa

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