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Rabbit Polyclonal YIPF6 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human YIPF6 aa 1-100.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-YIPF6 antibody (AB224224), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YIPF6 antibody (AB224224), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human YIPF6 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q96EC8

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IF
Human
Tested
Tested
Cow
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/50.00000 - 1/200.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

Fixation/Permeabilization: PFA/Triton X-100.

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Target data

Function

May be required for stable YIPF1 and YIPF2 protein expression.

Alternative names

Recommended products

Rabbit Polyclonal YIPF6 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human YIPF6 aa 1-100.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human YIPF6 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q96EC8
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

YIPF6 also known as YIP1 family member 6 is a protein involved in vesicular transport and membrane trafficking. It has a molecular mass of approximately 35 kDa. The protein localizes primarily at the Golgi apparatus and is expressed in various tissues particularly in those with high secretory functions such as the liver and pancreas. YIPF6 plays a significant role in maintaining Golgi structure and function by interacting with other Golgi-associated proteins.

Biological function summary

YIPF6 participates in the formation and maintenance of the Golgi complex. It functions as part of a larger protein complex collaborating with Rab Arf and other YIP family proteins to regulate vesicle budding and fusion. This activity is essential for proper protein and lipid trafficking within cells ensuring that the cellular export and import processes proceed efficiently. The protein's interactions with various co-factors highlight its role in sustaining intracellular logistics.

Pathways

YIPF6 is an essential component of the vesicular transport system and intracellular trafficking pathways. It closely associates with the COPI (coat protein complex I) pathway which mediates retrograde transport from the Golgi to the endoplasmic reticulum. YIPF6 also engages in related functions with proteins such as ARF1 a small GTPase critical for vesicle formation. These pathways highlight the protein's role in facilitating cellular transport mechanisms that are important for maintaining homeostasis within the cell.

Associated diseases and disorders

YIPF6's dysfunction can link to conditions involving protein misfolding and trafficking defects such as certain neurodegenerative diseases. Mutations or alterations in YIPF6 may also relate to metabolic disorders where liver and pancreatic functions are compromised. Its connection with proteins like Rab GTPases and ARF1 emphasizes its involvement in the underlying pathophysiology of these diseases as these associated proteins play roles in the same trafficking pathways that could lead to cellular stress and impairment when disrupted.

Product promise

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2 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-YIPF6 antibody (ab224224), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-YIPF6 antibody (ab224224)

    PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for YIPF6 (green) using ab224224 at 4 μg/ml in ICC/IF.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YIPF6 antibody (ab224224), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YIPF6 antibody (ab224224)

    Paraffin-embedded human epididymis tissue stained for YIPF6 using ab224224 at 1/50 dilution in immunohistochemical analysis.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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