Rabbit Polyclonal YKL-40/CHI3L1 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 26 publications.
Images
Publications
Filter by
- Scientific reports 13:191722023YKL-40 serum levels are predicted by inflammatory state, age and diagnosis of idiopathic inflammatory myopathies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMónica Vázquez-Del Mercado,Felipe Pérez-Vázquez,Ana L Márquez-Aguirre,Erika-Aurora Martínez-García,Efrain Chavarria-Avila,Carlos G Ramos-Becerra,Andrea Aguilar-Vázquez,Marisol Godínez-Rubí,Beatriz-Teresita Martín-Márquez,Livier Gómez-Limón,Guillermo Márquez-De-La-Paz,Edy-David Rubio-Arellano,Oscar Pizano-MartinezPubMed 37932407
- The Tohoku journal of experimental medicine 259:253-2612023The Potential Roles of CHI3L1 in Failed Autologous Arteriovenous Fistula in End-Stage Renal Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingge Zhang,Zhanghua Wang,Shasha Li,Xiaoxi Liu,Chennian Xu,Lu LiPubMed 36642504
- Oncology letters 25:362023Proteomic analysis of radioiodine-refractory differentiated thyroid cancer identifies CHI3L1 upregulation in association with dysfunction of the sodium-iodine symporter.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunjie Li,Fengqiong Hu,Jie Deng,Xin Huang,Chunyan Zhou,Mengxue Wu,Dong DuanPubMed 36589664
- Frontiers in oncology 12:9107282022Functional analysis of the short splicing variant encoded by CHI3L1/YKL-40 in glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengqi Shi,Qianyun Ge,Xinrong Wang,Wenbin Diao,Ben Yang,Sipeng Sun,Guohui Wang,Tian Liu,Andrew Man-Lok Chan,Zhiqin Gao,Yi Wang,Yubing WangPubMed 36408158
- Cell reports 41:1115112022A molecular interactome of the glioblastoma perivascular niche reveals integrin binding sialoprotein as a mediator of tumor cell migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYasmin Ghochani,Sree Deepthi Muthukrishnan,Alireza Sohrabi,Riki Kawaguchi,Michael C Condro,Soniya Bastola,Fuying Gao,Yue Qin,Jack Mottahedeh,M Luisa Iruela-Arispe,Nagesh Rao,Dan R Laks,Linda M Liau,Gary W Mathern,Steven A Goldman,S Thomas Carmichael,Ichiro Nakano,Giovanni Coppola,Stephanie K Seidlits,Harley I KornblumPubMed 36261010
- Theranostics 12:6989-70082022Chitinase-3 like-protein-1 promotes glioma progression via the NF-κB signaling pathway and tumor microenvironment reprogramming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Zhao,Jianming Zeng,Yujie Xu,Zhongping Su,Yulong Chong,Tao Ling,Haozhe Xu,Hui Shi,Minggao Zhu,Qi Mo,Xiaoying Huang,Yingchang Li,Xiaoren Zhang,Hongbin Ni,Qiang YouPubMed 36276655
- Nature communications 13:1812022Single cell transcriptomic landscape of diabetic foot ulcers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeorgios Theocharidis,Beena E Thomas,Debasree Sarkar,Hope L Mumme,William J R Pilcher,Bhakti Dwivedi,Teresa Sandoval-Schaefer,Ruxandra F Sîrbulescu,Antonios Kafanas,Ikram Mezghani,Peng Wang,Antonio Lobao,Ioannis S Vlachos,Biraja Dash,Henry C Hsia,Valerie Horsley,Swati S Bhasin,Aristidis Veves,Manoj BhasinPubMed 35013299
- Molecular therapy. Nucleic acids 27:699-7172022SPI1-induced downregulation of FTO promotes GBM progression by regulating pri-miR-10a processing in an m6A-dependent manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShouji Zhang,Shulin Zhao,Yanhua Qi,Boyan Li,Huizhi Wang,Ziwen Pan,Hao Xue,Chuandi Jin,Wei Qiu,Zihang Chen,Qindong Guo,Yang Fan,Jianye Xu,Zijie Gao,Shaobo Wang,Xing Guo,Lin Deng,Shilei Ni,Fuzhong Xue,Jian Wang,Rongrong Zhao,Gang LiPubMed 35317283
- International journal of oncology 60:2021Chitinase 3-like 1 secreted from cancer-associated fibroblasts promotes tumor angiogenesis via interleukin-8 secretion in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaori Watanabe,Kazuyoshi Shiga,Anri Maeda,Shinnosuke Harata,Takeshi Yanagita,Takuya Suzuki,Hajime Ushigome,Yuzo Maeda,Takahisa Hirokawa,Ryo Ogawa,Masayasu Hara,Hiroki Takahashi,Yoichi Matsuo,Akira Mitsui,Masahiro Kimura,Shuji TakiguchiPubMed 34913066
- Molecular oncology 16:2214-22342021Anti-Chi3L1 antibody suppresses lung tumor growth and metastasis through inhibition of M2 polarization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi Eun Yu,In Jun Yeo,Dong Ju Son,Jaesuk Yun,Sang-Bae Han,Jin Tae HongPubMed 34861103
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| IHC-P | ICC/IF | WB | |
|---|---|---|---|
| Human | Expected | Expected | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Orangutan | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.
Alternative names
Chitinase-3-like protein 1, 39 kDa synovial protein, Cartilage glycoprotein 39, YKL-40, CGP-39, GP-39, hCGP-39, CHI3L1
Recommended products
Rabbit Polyclonal YKL-40/CHI3L1 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 26 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
From May 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab255297.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Immunocytochemistry/ Immunofluorescence - Anti-YKL-40/CHI3L1 antibody (ab77528)
ab77528 staining Chitinase-3-like protein 1 precursor in U-87 MG cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab77528 at 5µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Western blot - Anti-YKL-40/CHI3L1 antibody (ab77528)
False colour image of Western blot: Anti-YKL-40/CHI3L1 antibody staining at 1 ug/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab77528 was shown to bind specifically to YKL-40/CHI3L1. A band was observed at 33-42 kDa in wild-type THP-1 cell lysates with no signal observed at this size in CHI3L1 knockout cell line Human CHI3L1 knockout THP-1 cell line ab280038 (knockout cell lysate Human CHI3L1 knockout THP-1 cell lysate ab280097). To generate this image, wild-type and CHI3L1 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-YKL-40/CHI3L1 antibody (ab77528) at 1 µg/mL
Lane 1: Wild-type THP-1 cell lysate at 20 µg
Lane 2: CHI3L1 knockout THP-1 cell lysate at 20 µg
Lane 3: U-87 MG cell lysate at 20 µg
Lane 4: Jurkat cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 33-42 kDa
Western blot - Anti-YKL-40/CHI3L1 antibody (ab77528)
All lanes: Western blot - Anti-YKL-40/CHI3L1 antibody (ab77528) at 1 µg/mL
All lanes: THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody (ab77528)
IHC image of YKL-40/CHI3L1 staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77528, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com