Skip to main content

Rabbit Recombinant Monoclonal YKL-40/CHI3L1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.


Images

Western blot - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (AB255864), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (AB255864), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (AB255864), expandable thumbnail
  • Western blot - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (AB255864), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (AB255864), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Not recommended
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.

Alternative names

Chitinase-3-like protein 1, 39 kDa synovial protein, Cartilage glycoprotein 39, YKL-40, CGP-39, GP-39, hCGP-39, CHI3L1

Recommended products

Rabbit Recombinant Monoclonal YKL-40/CHI3L1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.

Alternative names

Chitinase-3-like protein 1, 39 kDa synovial protein, Cartilage glycoprotein 39, YKL-40, CGP-39, GP-39, hCGP-39, CHI3L1

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR19078-157

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab255864 is the carrier-free version of Anti-YKL-40/CHI3L1 antibody [EPR19078-157] ab255297.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

YKL-40 also known as chitinase-3-like protein 1 (CHI3L1) is a glycoprotein with a molecular weight of approximately 40 kDa. Scientists often study it for its roles in inflammatory processes. It has expressions in numerous tissues including liver cartilage and the central nervous system. The CHI3L1 protein does not possess chitinase activity unlike other members of the chitinase family yet it interacts with various ligands to influence cellular behavior.

Biological function summary

YKL-40 plays significant roles in tissue remodeling and inflammation. It does not form part of a larger complex by itself but influences several cellular processes. This protein affects cell proliferation migration and survival making it involved in both normal physiological processes and pathological conditions. CHI3L1 also exhibits a connection to the immune response where it modulates macrophage activity and interacts with other immune cells.

Pathways

YKL-40 is integral to the inflammatory and extracellular matrix pathways. It associates with proteins such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) influencing signaling cascades that modulate inflammation. Through these interactions CHI3L1 becomes a mediator of connective tissue metabolism and immune reactions in various pathophysiological states.

Associated diseases and disorders

YKL-40 shows strong links to conditions like osteoarthritis and certain cancers. In osteoarthritis elevated levels of CHI3L1 reflect increased cartilage degradation while in oncology it correlates with tumor angiogenesis and metastasis potential. Its connection with proteins such as matrix metalloproteinases highlights its role in disease mechanisms highlighting its potential as a biomarker for early detection and disease progression monitoring.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (ab255864), expandable thumbnail

    Western blot - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (ab255864)

    False colour image of Western blot: Anti-YKL-40/CHI3L1 antibody [EPR19078-157] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-YKL-40/CHI3L1 antibody [EPR19078-157] ab255297 was shown to bind specifically to YKL-40/CHI3L1. A band was observed at 37/40 kDa in wild-type THP-1 cell lysates with no signal observed at this size in CHI3L1 knockout cell line Human CHI3L1 knockout THP-1 cell line ab280038 (knockout cell lysate Human CHI3L1 knockout THP-1 cell lysate ab280097). To generate this image, wild-type and CHI3L1 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (Anti-YKL-40/CHI3L1 antibody [EPR19078-157] ab255297) at 1/1000 dilution

    Lane 1: Wild-type THP-1 cell lysate at 20 µg

    Lane 2: CHI3L1 knockout THP-1 cell lysate at 20 µg

    Lane 3: U-87 MG cell lysate at 20 µg

    Lane 4: Jurkat cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 43 kDa

    Observed band size: 37 kDa, 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (ab255864), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (ab255864)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling YKL-40/CHI3L1 with Anti-YKL-40/CHI3L1 antibody [EPR19078-157] ab255297 at 1/250 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on neutrophils of human spleen is observed.

    The section was incubated with Anti-YKL-40/CHI3L1 antibody [EPR19078-157] ab255297 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-YKL-40/CHI3L1 antibody [EPR19078-157] ab255297).

  • Flow Cytometry (Intracellular) - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (ab255864), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (ab255864)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanolpermeabilized THP-1 (human monocytic leukemia cell line) cells labelling YKL-40/CHI3L1 with Anti-YKL-40/CHI3L1 antibody [EPR19078-157] ab255297 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-YKL-40/CHI3L1 antibody [EPR19078-157] ab255297).

  • Western blot - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (ab255864), expandable thumbnail

    Western blot - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (ab255864)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed in 293T and Jurkat are consistent with the literature (PMID: 19767768，PMID: 16472595).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-YKL-40/CHI3L1 antibody [EPR19078-157] ab255297).

    All lanes: Western blot - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (Anti-YKL-40/CHI3L1 antibody [EPR19078-157] ab255297) at 1/1000 dilution

    Lane 1: Human spleen tissue lysate at 20 µg

    Lane 2: THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lane 3: U-87 MG ( Human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: MG-63 (Human osteosarcoma fibroblast) whole cell lysate at 20 µg

    Lane 5: Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 6: 293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 7: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 43 kDa

    Observed band size: 27 kDa, 42 kDa

    Exposure time: 60s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (ab255864), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (ab255864)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling YKL-40/CHI3L1 with Anti-YKL-40/CHI3L1 antibody [EPR19078-157] ab255297 at 1/250 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on macrophages in human tonsil germinal center is observed. The section was incubated with Anti-YKL-40/CHI3L1 antibody [EPR19078-157] ab255297 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-YKL-40/CHI3L1 antibody [EPR19078-157] ab255297).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com